FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n01_c4_facs2_outgrowth.351000000b0bed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n01_c4_facs2_outgrowth.351000000b0bed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017396
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGAG151.26563E-4143.971591
CGTGGAG151.26563E-4143.971591
GATACTG151.26563E-4143.971592
TAGAGCG151.26563E-4143.971592
TGGAGGT151.26563E-4143.971591
GGGTGCT151.26563E-4143.971591
GACAGAG151.26563E-4143.971592
TCGCAGA151.26563E-4143.971591
ATGATGG151.26563E-4143.971591
TCCGCAG151.26563E-4143.971591
GTACAGA151.26563E-4143.971591
CGACGTG151.26563E-4143.971592
ATGGCTG151.26563E-4143.971592
AGATACG151.26563E-4143.971591
CGCGGGG151.26563E-4143.971591
TAGACTG151.26563E-4143.971591
CAGCCTG306.8394E-10143.971592
TCCTGAG151.26563E-4143.971591
CGTGATG151.26563E-4143.971592
CGTGACG151.26563E-4143.971591
Produced by FastQC (version 0.11.2)