FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n01_c4_facs1_outgrowth.351000000b0bd0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n01_c4_facs1_outgrowth.351000000b0bd0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044979
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAC100.0072430423143.974082
GGGGCAC151.265308E-4143.974081
CGGACAG400.0143.974083
TCACGGA100.0072430423143.974082
TCTGCCC400.0143.974082
GCCCCTC100.0072430423143.974081
CTGCTGT100.0072430423143.974082
CTGCTGG202.2072945E-6143.974083
GTCATGT100.0072430423143.974082
CATGGTC151.265308E-4143.974082
ATGGGTG202.2072945E-6143.974083
GTCATAG202.2072945E-6143.974083
GAGGATG100.0072430423143.974083
GTGCACA151.265308E-4143.974082
CATGGGG100.0072430423143.974083
TAGAGTC100.0072430423143.974081
CAGCGTC100.0072430423143.974082
CGGGTGG151.265308E-4143.974083
CATGGCG151.265308E-4143.974083
GAGGACG100.0072430423143.974083
Produced by FastQC (version 0.11.2)