FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n01_ancestor_barcode_techrep2.351000000b0c8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n01_ancestor_barcode_techrep2.351000000b0c8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905818
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCG650.0143.971424
GGGCTCG500.0143.971424
CATGCCC253.8722646E-8143.971423
ATGGCCG750.0143.971424
GAGTCCG750.0143.971424
ACGCACG1000.0143.971424
CTGAGTC253.8722646E-8143.971423
TAGGTCG650.0143.971424
GGCGTCG750.0143.971424
TAGCGAC253.8722646E-8143.971423
CTCCCTG253.8722646E-8143.971422
AGCCACC253.8722646E-8143.971423
TCCCTAG253.8722646E-8143.971421
ACACAGC253.8722646E-8143.971423
AGGACCC253.8722646E-8143.971423
GATCACG650.0143.971424
GGTCACG650.0143.971424
GCTGATC253.8722646E-8143.971423
GGATAGC253.8722646E-8143.971423
CTGACCT253.8722646E-8143.971422
Produced by FastQC (version 0.11.2)