FastQCFastQC Report
Fri 18 Aug 2017
000000000-B4MYC_l01n03.3330000009a542.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4MYC_l01n03.3330000009a542.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14645748
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGTTGAAAGTTTTATTATTGTTATAATAAGATTACATTCATTACATT9883496.748368195328775No Hit
CGTTGTTGAAAGTTTTAACTATTATATAGTACTCAGACGACATTAATAAA2885981.970524141204669No Hit
CGTTGTTAAAAGTTTTAATTTATTAATTAAGTTTACTCAGACTGCAAAGT2772781.8932320834688674No Hit
CGTTGTTGAAAGTTTTATTATGTTATTAAAACGTTTACATTCATAAGACT2525671.724507345066978No Hit
CGTTGTTGAAAGTTGTAACTATTAAGTTTTTTCAGACGCTGATTGCAACT2523891.7232919752545244No Hit
CGTTGCTGAAAGTTTTGTTTTTATTTATGCTCAATTAAGAGACTAGTACA2445441.6697269405427433No Hit
CGTTGCTGAAAGTTTTATAAAACTTTTAATATTTCAACAGCTTAACTGCT2109391.4402746790399508No Hit
CGTTGCTGAAAGTTTTATTAAACTTTTAATATTTCAACAGCTTAACTGCT1555251.0619123038304359No Hit
CGTTGTTGAAAGTTTTGAAGATTTTTTGAATTTAATCAACAAATTGACAA1296880.8854993271767342No Hit
CGTTGTTAAAAGTTTTGTTTTTGTTATGATATTACATTCATTACTAACAG1197310.8175137248025843No Hit
CGTTGTTGAAAGTTGTAATTATTAATTTGTTACTGACGCTGATTGCAATT1092960.7462643765275765No Hit
CGTTGTCGAAAGTTGTATTAACTTTTTAGGGTCTGTCAAGACCATGATTA986140.6733285319397821No Hit
CGTTGTTGAAAGTTTTAATTATTGTTTAACTAAAAACTCAGACTGCAAAC946990.6465972239861016No Hit
CGTTGTTGAAAGTTTTGACTATTAGTAATAATCTGGTGTGACAAGTTGAT917210.6262636773485383No Hit
CGTTGTTGAAAGTTTTGAAGATTCGTATATCACTCGTCCTGCCACAGCGC877370.5990612428945248No Hit
CGTTGTTGAAAGTTTTGAAGATTAATTCAAAATTTGACTATCAATAAAAA877300.599013447452462No Hit
CGTTGTTGAAAGTTATATTAGTTTATAACGATTTTACATTCAATAACTTG861990.5885599014813037No Hit
CGTTGTTGAAAGTTTTATTTTGTTATAAAATTTAATACATTCATAGACTT757890.5174812512136628No Hit
CGTTGTTGAAAGTTTTGACTATTAAATAATCGGTTGACATTAAATAAAAT713980.4874998531997137No Hit
CGTTGCTGAAAGTTGTATATAAGATGCGTTACATCGCAATACACATTCTG708580.48381277624058533No Hit
CGTTGTTGAAAGTTTTGAAGATTAATGTTTTGGTGTTACCCAAACAAAAA691740.4723145584643406No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACTAAA689070.4704915037456605No Hit
CGTTGTTAAAAGTTTTGACAGTTCGCTAAGAACACTCAGAAGTATCGTCG654290.4467439969607561No Hit
CGTTGTTGAAAGTTATATTAGTTTATAACGATTTTACATTCAATGACTTG632940.4321663871316098No Hit
CGTTGTTGAAAGTTTTGATTTATTTGTTTTTTATACTCAGAAGTTCCACT620830.4238977756547498No Hit
CGTTGTTGAAAGTTATTTTTGTTATAATAACATGACGTTCATTACACAAT614720.419725916354699No Hit
CGTTGTTGAAAGTTTTTTTTGTTTTATTTTTATATACATACTTTAACTAA603080.411778217131689No Hit
CGTTGTTGAAAGTTTTGAAGATTAAAATTCAAATTGACTAGTTTCTAGAG578730.395152231214138No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTAATCTCAGACTACAATCTT562970.38439142882971905No Hit
CGTTGTTGAAAGTTTTAACGATTTTTATTATCGGTACTCAGACTGCAACC554450.37857404073864986No Hit
CGTTGTTGAAAGTTTTGAAGATTTATTTGAAATTAATCAACAAATTGACA551890.37682609314321125No Hit
CGTTGTTGAAAGTTTTATTGTTGTTATAATAAGATTACATTCATTACATT526130.35923737046411014No Hit
CATTGTTGAAAGTTTTTTTATGGTTTGTATAAAACAAAAATCAGACAATA505600.34521965009912775No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTTCACTCAGACTTCAATCTT503710.34392917316343286No Hit
CGTTGTTGAAAGTTTTGTTTTGTTATAAATTTGTATACATTCATAGACTT498430.34032403124784066No Hit
CGTTGTTGAAAGTTTTATTTTGTTATAATAAAACGACGTTCATTACACAT495400.338255171398552No Hit
CGTTGTTGAAAGTTATTATGTTTAATGGGCGACTGGGTCGCTGAACATTC475240.32449008408447283No Hit
CGTTGTTGAAAGTTTTGAAGATTATTTTGAAATTAATCAAACAAATTGAC468330.31977199116084754No Hit
CATTGTTGAAAGTTTTGATTATTATCAAGACACTCAGACAGTCCATCTTC462480.31577765778845845No Hit
CGTTGTTGAAAGTTTTATTTTTGTTATAATAAGATTACATTCATTACATT438120.29914484395061286No Hit
CGTTGTTGAAAGTTTTGATTGATTTATCATTTGACTCGGACGCACAGATG428740.292740254714201No Hit
CGTTGTTGAAAGTTTTGACTATTAAGTAATAATCTGGTGTGACAAGTTAA424200.28964037890041533No Hit
CGTTGTTAAAAGTTTTAATTTATTAATTAAATTTACTCAGACTGCAAAGT361650.24693173745717872No Hit
CGTTGTTGTAAGTTTTATTATTGTTATAATAAGATTACATTCATTACATT361250.2466586206453914No Hit
CGTTGCTGAAAGTTTTATTTTTGTTTATGCTCAATTAAGAGACTATTACA340680.2326135885992303No Hit
CGTTGTTGAAAGTTTTTAATATTTTAAAATTTCCAGTTACGAAAATTCTT338870.23137773502589284No Hit
CGTTGTTGAAAGTTGTAACTATTATGTTTTTTCAGACGCTGATTGCAACT327650.22371680845525954No Hit
CGTTGCTGAAAGTTGTATAATAATTTTCATAGGCATAGCCCATGTAAAGA321680.21964054003933428No Hit
CGTTGTTGAAAGTTTTAATTTATTAATTAAATTTACTCAGACTGCAAAGT315480.21540722945663135No Hit
CGTTGCTGAAAGTTTTGATTTATTTGCTTATGCCACTCAGAAATCCACTG286700.19575647484853625No Hit
CGTTGTTGAAAGTTTTAACGATTTTTTATATCGGTACTCAGACTGCAACC284940.1945547608766722No Hit
CGTTGTTGAAAGTTTTGATTTATTCAAAATTTTAACTCAGACGACCGGTT284670.19437040702871577No Hit
CGTTGTTGAAAGATATTATGTTTAATGGGCGACTGGGTCGCTGAACATTC279830.19106569360608963No Hit
CGTTGTTGAAAGTTTTGACTATTGTAATAATAAATCAAGTTTGACTGTAA264090.18031854706225997No Hit
CGTTGTTGAAAGTTTTATTATTGTTATATAAGATTACATTCATTACATTT259090.17690458691491892No Hit
CGTTGTTGAATGTTTTATTATTGTTATAATAAGATTACATTCATTACATT250480.17102574754119762No Hit
CGTTGTTGCAAGTTTTATTATTGTTATAATAAGATTACATTCATTACATT232310.1586194163657602No Hit
CGTTGCTGAAAGTTTTATAAATCTTTTAATATTTCAACAGCTTAACTGCT230130.15713092974151951No Hit
CGTTGTTGAAAGTTTTAACGATTTTTATATCATTCACTCAGACTGCAACC228920.15630475138586297No Hit
CGTTGTTGATAGTTTTATTATTGTTATAATAAGATTACATTCATTACATT214800.14666372792977184No Hit
CGTTGTTGAAAGTTTTGACTTATTAAATAAGACACTCAGATAATCAACTA200560.13694076943014452No Hit
CGTTGCTGAAAGTTGTATTTATTTGCTTATTATGCAAGGTTTACATTCTG195260.13332197167396298No Hit
CGTTGTTGAAAGTTTTGACTATTAGTTAATCAGTTGACTTAAATAAAAAT183040.12497825307386144No Hit
CGTTGCTGAAAGTTTTATTAATCTTTTAATATTTCAACAGCTTAACTGCT178370.1217896142962449No Hit
CATTGTTGAAAGTTTTAACTGATTGCATTCAATCAACTCAGACTGCACGC174990.11948177723664233No Hit
CGTTGTTGACAGTTTTATTATTGTTATAATAAGATTACATTCATTACATT166660.11379411963117214No Hit
CGTTGTTGAAGGTTTTATTATTGTTATAATAAGATTACATTCATTACATT163780.11182767858630369No Hit
CATTGTTGAAAGTTTTGACTGATTTTATATTCAGACTCAGACTGCATCAC162480.110940048947995No Hit
CGTTGTTGAAAGTTGTATTAGATGCGTTTTATTACATCGAGTAGACATTC156270.10669991044499742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAGT9948800.0244.056817
GAAAGTT10047550.0243.936848
TTGAAAG8585950.0243.810296
GTTGAAA8595150.0243.291345
TGTTGAA8921600.0242.433884
GCTGAAA1447200.0242.374745
CTGAAAG1453350.0242.031856
AAAGTTT9182400.0236.319349
TTGTTGA9423000.0236.232833
CGTTGTT10088900.0236.060561
GTTGTTG9552450.0234.890992
CGTTGCT1545750.0231.478841
AAAGTTG1643400.0227.504189
TCGAAAG197100.0220.200556
TGCTGAA1651000.0214.993474
GTTGCTG1731650.0210.745772
TTGCTGA1742700.0205.121613
GTCGAAA218300.0198.479315
CGAAAGT234100.0189.688267
GTTGAAT203850.0189.593115
Produced by FastQC (version 0.11.2)