FastQCFastQC Report
Fri 18 Aug 2017
000000000-B4MYC_l01n02.3320000009a545.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4MYC_l01n02.3320000009a545.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14645748
Sequences flagged as poor quality0
Sequence length12
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTCTATTGT4844453.3077518471572773No Hit
TACACGATCTAC3890222.6562112088778256No Hit
CTAGCGAACATC3704072.529109472592318No Hit
GTCTACACACAT3620212.4718505330011142No Hit
ATCCCGAATTTG3104852.1199668326943764No Hit
GACTCGAATCGT2890941.9739107896708314No Hit
ATACTTCGCAGG2728041.8626839680704597No Hit
TAGCTCGTAACT2612241.783616651058041No Hit
GGATCGCAGATC1870821.2773809845697195No Hit
GCACACACGTTA1868451.2757627674598797No Hit
CCATAGGGTTCA1782131.216824159476184No Hit
CGGACTACAACT1685931.1511395662413417No Hit
AACACAAGGAGT1620071.1061708831805654No Hit
GGTCACTGACAG1599851.0923648283447183No Hit
GAGGCTCATCAT1504711.0274039946611124No Hit
TGAGCCGGAATC1484821.0138232611949898No Hit
TAACGCTTGGGT1396190.9533074036232223No Hit
GACACATTTCTG1289230.8802759681513024No Hit
CTAACCTCCGCT1220170.8331223505962276No Hit
CTCTACCTCTAC1217020.8309715557034028No Hit
ATACCTTCGGTA1213900.828841244571462No Hit
TGTAATTGTCGC1201280.8202244091595732No Hit
CACGCCATAATG1163290.7942851399600758No Hit
AGCTGTTGTTTG1141660.7795163483626784No Hit
ATGGACCGAACC1064030.7265112031150611No Hit
ATGTGCACGACT1052590.7187000622979447No Hit
TATCAGGTGTGC1011830.6908694591768205No Hit
GAAGTTGGAAGT1009830.689503875117884No Hit
TTGCGCATACTA1005500.6865473856302867No Hit
GCTCAGTGCAGA1003150.6849428243610364No Hit
GGTCAGCTTAAC1000680.6832563280482499No Hit
AATCAGTCTCGT994150.6787976960958225No Hit
CGGAGCTATGGT992240.6774935633195381No Hit
TCTTCCGCTACT979200.6685899552552726No Hit
TACTAATCTGCG970710.6627930509250877No Hit
TAATCCACAGCG960780.6560129260724683No Hit
CGCAGCGGTATA955460.6523804724756973No Hit
TCGAGGACTGCA947950.6472527043343912No Hit
TCCTCTGTCGAC944690.6450268023183248No Hit
GCACGACAACAC941800.6430535333531616No Hit
ATTCCTGTGAGT937290.63997414130026No Hit
TATGCACCAGTG915980.6254238431522924No Hit
GTCAATTGACCG892970.6097127985542289No Hit
CGACTGTCTTAA891500.6087090942709106No Hit
GACTTTCCCTCG885760.604789868021763No Hit
AGGCTACACGAC871230.5948688998335899No Hit
GGAAACCACCAC866800.5918441311430458No Hit
TCACGGGAGTTG865470.5909360177438531No Hit
TAGGAACTGGCC862870.5891607584672357No Hit
GAACTAGTCACC853280.5826127829046356No Hit
CCTGAACTAGTT851640.5814930039763077No Hit
ACACGTAAGCCT849440.5799908615114776No Hit
TCGTCGATAATC846610.5780585600680825No Hit
TCCCAGAACAAC836700.5712920910560526No Hit
GAGTGGTAGAGA833710.5692505428879426No Hit
ACACCTGGTGAT822890.5618627331290966No Hit
TTCCTAGGTGAG812880.5550279849141198No Hit
GCTGATGAGCTG809950.553027404267778No Hit
CACGGTTGTGAG809440.5526791803327491No Hit
AAGAGATGTCGA807230.5511702099476243No Hit
GTCATATCGTAC798420.5451548121680094No Hit
TCCGACACAATT773770.528323988641618No Hit
GACGGAACCCAT773360.5280440439095361No Hit
GACAGGAGATAG768960.525039758979876No Hit
CAAGCATGCCTA766600.523428369790331No Hit
GAATCTTCGAGC740800.5058123354300511No Hit
TATCGTTGACCA736730.5030333718701154No Hit
TGAGTCACTGGT733290.5006845672887448No Hit
TTACTGTGCGAT721270.4924774070945369No Hit
GATTGGTTGCAC702240.47948387477375687No Hit
GGATGGTGTTGC699780.47780420638126503No Hit
ATTAGTTCGCGT695340.47477260977042623No Hit
CAGCTAGAACGC673410.45979898056418833No Hit
GAAGAAGCGGTA655420.4475155519540552No Hit
ATGTGGGACCCA651880.44509846816973775No Hit
ATCGATCTGTGG632210.431667948950098No Hit
AATCCGTACAGC624040.4260895380693427No Hit
CCATACATAGCT618530.42232735398697285No Hit
AAGGAGCGCCTT610180.4166260405409133No Hit
TCCAAAGTGTTC598780.4088422114049757No Hit
ATGAGACTCCAC581490.39703673721547034No Hit
CGGGTTTGACGA579680.3958008836421329No Hit
GGAGACAAGGGA569100.3885769439703592No Hit
GTACATACCGGT558800.3815441860668366No Hit
CTTCGGCAGAAT550610.37595211934549194No Hit
CTATTTGCGACA539650.36846871870252035No Hit
CCAGTGTATGCA534490.36494551183046436No Hit
TAGGATTGCTCG532890.36385304458331524No Hit
AGGCATCTTACG532640.36368234657594817No Hit
AGATGTTCTGCT529620.3616203146469542No Hit
TACAGATGGCTC528290.3607122012477615No Hit
CAACACGCACGA525130.35855457843464195No Hit
AGCTGGAAGTCC513510.3506205350522213No Hit
TTATGCAGTCGT500620.34181934579237605No Hit
TGCAATGTTGCT496450.33897210302949365No Hit
GTCTAATTCCGA469820.3207893512847551No Hit
TTAGGGCTCGTA469100.32029774102353803No Hit
ACTCACGGTATG464970.3174778099418343No Hit
TCTAGCGTAGTG447320.30542653062172037No Hit
ACGCGCAGATAC393280.26852844934925824No Hit
ACGGGACATGCT381440.2604441917203546No Hit
TCAAGCTCAAGC296940.20274826523029071No Hit
TTTTTTTTTTTT278040.18984349587334154No Hit
ATCGGCGTTACA275090.1878292593864103No Hit
CCTCGTTCGACT272100.18578771121830034No Hit
GCGGCAATTACG242860.16582287227664985No Hit
CTGCTAACGCAA202640.1383609768514384No Hit
ACATTCAGCGCA194710.13294643605775547No Hit
GCATGGCTCTAA193090.13184031297001694No Hit
ATCGCTCGAGGA158040.10790845233715615No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCACG3750.06.0069061
GTCCCGA650.0012446536.0069061
GACGCGA600.00335318646.0069061
CCATTGG908.983468E-66.00690561
GGCAACC751.7240079E-46.0069051
ATCCCGA320050.06.00315241
GACTCGA312150.06.00209471
CGGACTA185450.06.0020471
GGTGTCT513200.05.99988271
CCATAGG196000.05.99924371
CGTTGCC650.0012654385.9986214
CCAATCG650.0012654385.9986215
ACCAGCG1156.858863E-85.9986216
AGCGAGC806.561082E-55.9986213
CGGTGAC2250.05.9986214
CATCGGG953.443176E-65.9986212
GCAGCTC2600.05.9986216
CTCCTGG650.0012654385.9986212
CGACTCG1451.9645086E-105.9986215
CCGTGCA600.00340525275.99862054
Produced by FastQC (version 0.11.2)