FastQCFastQC Report
Fri 18 Aug 2017
000000000-B4MYC_l01n01.3310000009a548.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4MYC_l01n01.3310000009a548.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14645748
Sequences flagged as poor quality0
Sequence length251
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTCGGTCCAT208611314.243813289700192No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTAATTATTGAGTGTAAAA5938724.054910681243458No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTTTAGGACGTTCTTTTT3456522.3600843056974625No Hit
GTAGGTGAACCTGCGGAAGGATCACTAGTGATTAAATCGAGCGTGTCTTC3217142.196637549683362No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGGGTA2931432.0015570389439996No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACCACC2877081.964447292142402No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTT2838691.9382349061311175No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTTGCCTG2638271.8013897275850985No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGGTCC2597311.7734225660580805No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCC2576671.7593297385698565No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACCACA2498551.7059900252278No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTGCCAGCGCTTA1742211.1895671016598128No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAGAAACAAAAAGCTGTCTCGTG1524941.0412168774172545No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGACCCCTCGCGGGT1490181.0174830264729393No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGTTTGGGCGCCCCTTCT1316100.8986225899831132No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAATCAAACAAGTTCATCTTG1281090.874718041031431No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTTGAAACGGGTTGTAG1229270.8393357580643884No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTATTGCCTGCGCTTA1208170.8249288462426091No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGCGGGCTGCCTCCGGGC1194180.8153765857503489No Hit
GTAGGTGAACCTGCGGAAGGATCACTAAAGAATTCGCCCTTCGGGGCTCT1152570.7869656094041765No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGTATCTCGGGCGCTCGACCGA1093910.7469130289555713No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAAGTGCTTAACTGC1054370.7199154321103982No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATAGTGCCCTCTGATGCAAATCA1004690.6859943240864175No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAACTTA994850.6792756505164502No Hit
GTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCTGGA988370.6748511581654962No Hit
GTAGGTGAACCTGCGGAAGGATCAGTAGTGAATATTGGACTTCGGTCCAT954550.6517591317288813No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGATTAACTGTCTGTCGAGCTT952810.6505710735976066No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAGAACCTGGGCCTGCGCTACTT952580.650414031430829No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTCCTTTGGGCCC912060.622747298396777No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTTGTGGACTTCGGTC890450.6079921626399689No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGTTTGGATGCCCCTTCT855140.5838827760794464No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGAGCGTTAA811710.5542291182396419No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCC779900.5325095037822583No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATTTAGAGCGTAACTTCGG718760.4907635991005717No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATCTAGGACGTCCAACTTA700040.47798173230892677No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTGCCTTTATAGGCTTATA694610.4742741715889144No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTTATTATATTCTACCAGCGC679520.4639708398642391No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGTTTGGGCGCCCCCTCG570680.38965575537691893No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTAATTATTGAGTGATAAA567920.3877712493755867No Hit
GTAGGTGAACCTGCGGAAGGATCATTGCCGTGACCCTTAAACAAAACAGA564700.38557265904069904No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAAGTCTGGGCTTTGGCCCTC557430.3806087609864651No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAA533930.3645631482939622No Hit
GTAGGTGAACCTGCGGAAGGATCACTAGAGATTTCGCCCTCCGGGGCTCT493040.336643782209007No Hit
GTAGGTGAACCTGCGGAATGATCATTACAGAGTTCATGCCCGAAAGGGTA491110.33532599359213333No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTTTGGGTCC483060.32982951775491426No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAATTATGTTTTCTAGATGGG470690.3213833803503925No Hit
GTAGGTGAACCTGCGGAGGGATCATTACCGAGTGAAGGGCCTCTGGCCCG442350.3020330542352634No Hit
GTTGGTGAACCAGCGGAGGGATCATTACTGAGTACTACACTCTCTACCCT434360.29657754591981234No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGAGTAAGGGTGCTCAGCGCCC431900.29489787752732055No Hit
GTAGGTGAACCTGCGGAAGGATCACTAAAGTAAACGCCCTCCGGGGCTCT413770.28251885803306187No Hit
GTAGGTGAGCCTGCGGAAGGATCAGTAGAGAATATTGGACTTCGGTCCAT355460.24270525479477048No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTCCCTCGTGGCC318480.217455605545036No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTTGACCCCGGCCCTCGGGC296230.2022634828893683No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAGAATATTGGACTTCGGTCCAT277790.1896727978659745No Hit
GTTGGTGAACCAGCGGAGGGATCATTACTGAGTACCACACTCTATACCCT276350.18868957734354025No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAGTGCTTAACTGCA259160.1769523823569817No Hit
GTTGGTGAACCAGCGGAGGGATCATTACTGAGTGTAAAAACTCCCAAACC254800.1739754091085003No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCCTCGTGGCC247230.16880667344542594No Hit
GTAGGTGAACCTGCGGAAGGACCAGTAGAGAATATTGGACTTCGGTCCAT245130.1673728101835427No Hit
GTAGGTGAACCTGCGGAAGGATCACTAGTGATTAAATCTAGCGTGTCTTC223450.15256987898467186No Hit
GTAGGTGAACCTGCGGAGTGATCATTACAAGTGACCCCGGTCTAACCACC221360.1511428436430833No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAAATTATGACTGGGGTTGTA220470.1505351587368566No Hit
GTAGGTGAACCTGCGGAAGGGTCAGTAGAGAATATTGGACTTCGGTCCAT206410.14093510280253355No Hit
GTTGGTGAACCAGCGGAGGGATCATTACAGAGTTATCCAACTCCCAAACC195470.1334653580001513No Hit
GTAGGTGAACCTGCGGAAGGATCAGTAGGGAATATTGGACTTCGGTCCAT188930.1289998981274292No Hit
GTAGGTGAACCTGCGGAAGGACCAGTAGAGAATATTAGACTTCGGTCCAT180280.12309374707252918No Hit
GTAGGTGAACCTGCGGGAGGATCATTAGTGATTAATTATTGAGTGTAAAA178810.12209004278921089No Hit
GCAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTCGGTCCAT174560.119188176663971No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTATTGCCAGCGCTTA164420.11226466548516334No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAGTTTTGAAATGGGTTGTAG162170.11072838341885985No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTTGTAGACTTCGGTC158830.10844785804043605No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTAAATGCGAAGCCTCACGGCCT158530.10824302043159557No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGATAGTTTTAGTTTGTTGCCT149860.10232321353610618No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAATAACTATGGCGTTGGTTG148930.10168821694870075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGAA12449800.0244.934083
TGAACCT12581150.0244.574776
GGTGAAC12876150.0244.29054
TAGGTGA12560650.0243.585042
GTAGGTG12631300.0243.449661
GAACCTG12715550.0242.582147
ACCTGCG12856550.0240.563929
AACCTGC12832900.0240.351448
GTGAACC13209150.0238.77565
TTGGTGA360250.0224.502982
TGGTGAA368300.0219.928673
GTTGGTG372050.0218.469361
GGACCTG58350.0217.917117
GAACCAG367650.0214.878337
GGAGGTG66750.0213.841031
AACCAGC372400.0211.907278
AACCCGC49400.0204.827358
AGGTGGA61700.0204.336793
TGAACCA388650.0202.952586
GACCTGC63000.0202.416068
Produced by FastQC (version 0.11.2)