FastQCFastQC Report
Thu 3 Aug 2017
000000000-B4HVB_l01n03.33300000098eef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4HVB_l01n03.33300000098eef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17907552
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGTTGAAAGTTGTAATTATTAATTTGTTACTGACGCTGATTGCAATT2793051.5597050897855833No Hit
CGTTGTTGAAAGTTTTGAAGATTAATGTTTTGGTGTTACCCAAACAAAAA2622591.4645161996458256No Hit
CGTTGTTGAAAGTTTTATTATTGTTATAATAAGATTACATTCATTACATT2067181.1543621372703539No Hit
CGTTGCTGAAAGTTGTATTTTGTTTTATAGGCTTAAAGCCCATTGACTAC2008121.1213816383166164No Hit
CGTTGCTGAAAGTTGTATAAAGATGCGTTATACGCAGATAAACATTCTAT1700740.9497333862272186No Hit
CGTTGTTGAAAGTTGTAACTATTATGTTTTTTCAGACGCTGATTGCAACT1302970.7276092231925391No Hit
CGTTGTTGAAAGTTTTAATTTATTAATTAAATTTACTCAGACTGCAAAGT1170710.6537521153086698No Hit
CGTTGTTAAAAGTTTTAATTTATTAATTAAGTTTACTCAGACTGCAAAGT1089080.6081679952681416No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACAATA882730.4929372814329955No Hit
CGTTGTTGAAAGTTTTGAAGATTCGTATATCACTCGTCCTGCCACAGCGC876480.48944713381259486No Hit
CATTGTTGAAAGTTTTGATTATTATCAAGACACTCAGACAGTCCATCTTC872880.4874368087832441No Hit
CATTGTTGAAAGTTTTGACTGATTTTATATTCAGACTCAGACTGCATCAC816610.4560143117272534No Hit
CGTTGTTGAAAGTTTTGAAGATTAAAATTCAAATTGACTAGTTTCTAGAG725050.4048850451474328No Hit
CGTTGTTGAAAGTTATTTTTGTTATAATAACATGACGTTCATTACACAAT602670.3365451626218927No Hit
CGTTGTTGAAAGTTTTGGAGATTAATGTTTTGGTGTTACCCAAACAAAAA550310.30730610191722463No Hit
CGTTGTTGAAAGTTTTAATGATTCAATATCCACTCAGACTCACTATTCAG549090.30662482510172245No Hit
CGTTGCTGAAAGTTGTATATAGATGCGTTATACGCAAGGTTACATTCTAA525610.2935130385214014No Hit
CGTTGTTGAAAGTTGTAACTATTAAAGTTTTCTGACGCTGATTGCAATTA509880.2847290349903772No Hit
CGTTGTTGAAAGTTTTGAAGATTTTTTGAATTTAATCAACAAATTGACAA503130.28095967556034457No Hit
CGTTGCTGAAAGTTGTATTTAGATGCGTTACATCGCGATACACATTCTGT483260.269863798245567No Hit
CGTTGCTGAAAGTTTTATTAAACTTTTAATATTTCAACAGCTTAACTGCT482960.2696962711597878No Hit
CGTTGTTAAAAGTTTTAAAATTTTTGTTTTCATTCAGACTTATAGTTAGG457160.2552889417827741No Hit
CGTTGCTGAAAGTTGTATTATATGCTTTTACACATGATAATGACATTCTA452550.2527146088979666No Hit
CGTTGCTGAAAGTTGTATATTTGTGTTATACACAACAAAACATTCTATAA443670.24775580715890147No Hit
CGTTGTTGAAAGTTTTGACTATTGTAATAATAAATCAAGTTTGACTGTAA440460.2459632673410637No Hit
CATTGTTGAAAGTTGTAACGATTGTGTTTTGTATCAGAACAGGTAGTTAT436730.24388034724120863No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACAGAA426380.23810066278182523No Hit
CGTTGTCGAAAGTTGTATTAACTTTTTAGGGTCTGTCAAGACCATGATTA422950.23618526976774937No Hit
CGTTGCTGAAAGTTGTATTTCATGCGTTAGACGCAAATGTGACATTCTAA399530.22310698860458425No Hit
CGTTGTTGAAAGTTGTATTTGTTATAATAAATTACATTCTTTACACTGAT398480.22252064380435696No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACTAAA390830.2182487031169866No Hit
CGTTGTTGAAAGTTTTTAATATTTTAAAATTTCCAGTTACGAAAATTCTT369080.20610298939799254No Hit
CGTTGCTGAAAGTTGTATAGTTTTAAAGGGCCAGTTAAGTACCCAATTAA353160.19721288537930814No Hit
CGTTGTTGAAAGTTTTAATTATTGTTTAACTAAAAACTCAGACTGCAAAC339300.18947313401630775No Hit
CGTTGTTGAAAGTTGTATTAGATGCTTTTTATCGCATAGTAGACATTCAT324110.18099067923968615No Hit
CGTTGTTGAAAGTTTTATTGTTGTTATAATAAGATTACATTCATTACATT319130.17820972961575093No Hit
CGTTGTTGAAAGTTGTAACTATTATGTTTTTCTGACGCTGATTGCAATTA317130.17709288237722276No Hit
CGTTGTTGAAAGTTTTGACTATTAGTTAATCAAGTTGACAATTAATAAAA311400.17389311503883947No Hit
CATTGTTGAAAGTTTTGACTGTTTTTATATTCAGACTCAGACTGCATCAC300640.1678844768955578No Hit
CGTTGCTGAAAGTTGTATATAGATGCGTTACATCGCAATACACATTCTAA295820.16519287505070487No Hit
CATTGTTGTAAGTTTTGACTGATTTTATATTCAGACTCAGACTGCATCAC288650.1611889777005813No Hit
CGTTGTTGAAAGTTTTTTTTGTTTTGTTTTTATATACATACTTTAACTAA286430.159949277265815No Hit
CGTTGCCGAAAGTTGTATTATAACTTTATGCTCAATTAAGAGACTTTATA282770.15790544681930843No Hit
CGTTGTTGAAAGTTTTAACTGATTCAAAAAATCATCCTCAGAGGTGCCAC277390.1549011277476676No Hit
CGTTGTTGAAAGTTTTGAAGATTATTGTTTTGGTGTTACCCAAACAAAAA276600.15445997308844894No Hit
CGTTGCTGAAAGTTTTATAAAACTTTTAATATTTCAACAGCTTAACTGCT276560.1544376361436784No Hit
CGTTGTTGAAAGTTTTGAAGATTTATTTGAAATTAATCAACAAATTGACA262330.1464912680415503No Hit
CGTTGCTGAAAGTTGTATATAGATGCGTTAACATCATAATAAACATTCTG261300.14591609171370828No Hit
CATTGTTGAAAGTTGTAATTTTGTTTGTTTCTGTACTGAATTGACTGTAA251360.14036536093822316No Hit
CGTTGTTGAAAGTTGTAATTATTATTTTGTTACTGACGCTGATTGCAATT236530.13208393866453663No Hit
CGTTGTTGAATGTTTTGAAGATTAATGTTTTGGTGTTACCCAAACAAAAA235820.1316874578948591No Hit
CGTTGTTGAAAGTTTTGAAGATTATTTTTGAAATTAATCAAACAAATTGA222580.12429392917580248No Hit
CGTTGCTGAAAGTTGTATTACAATTTTCATAGGCATAGAAGCCCATGTAA213340.11913409493380224No Hit
CGTTGCTGAAAGTTGTATATAAATGCGTTATACGCAGAGTAACATTCTTT211450.11807867429339308No Hit
CGTTGTTGAAAGTTTTGACTATTAGTTAATCAAGTTGACAATTAAATAAA201640.1126005385884123No Hit
CGTTGTTGAAAGTTTTAACGATTGTTTAACTAAAAACTCAGACTGCAAAC191270.10680968565664364No Hit
CGTTGCTGAAAGTTGTATTTAATTTGCTCGTTAGCAATGATGACATTCTT190330.10628476745453538No Hit
CGTTGTTGAAAGTTTTGACTTATTAAATAAGACGCTCAGATAATCAACTA182160.10172244648514771No Hit
CGTTGTTGAAAGTTTTGACTATTTTGTAATAATAAATCAAGTTTGACTGT181880.10156608787175378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAGT10620250.0243.307837
GAAAGTT10663500.0242.919078
TTGAAAG8985650.0241.645166
GCTGAAA1672750.0241.53565
GTTGAAA8986900.0241.309595
TGTTGAA9842200.0240.760274
CTGAAAG1682850.0240.500216
AAAGTTG4316350.0234.064519
TTGTTGA11122200.0233.635253
AAAGTTT7116600.0228.932639
CGTTGCT1872250.0228.76281
CGTTGTT11607150.0221.52431
TGCTGAA1883550.0220.86444
TTGAACG384100.0213.201926
TGAACGT405350.0213.116017
GCAAGTT507350.0208.323218
GTTGTTG12487050.0206.034592
TTGCTGA2136200.0201.767323
CATTGTT2225100.0200.387131
TGCAAGT544900.0195.585397
Produced by FastQC (version 0.11.2)