FastQCFastQC Report
Thu 3 Aug 2017
000000000-B4HVB_l01n02.33200000098ee2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4HVB_l01n02.33200000098ee2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17907552
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAAACACCCG15369968.582948691144384No Hit
TATGCACCAGTG8163634.558763810932952No Hit
CTGCTAACGCAA3991622.229014887126951No Hit
TCGTCGATAATC3470871.9382157873951726No Hit
CCTGAACTAGTT2725861.5221846068072287No Hit
ACTATTGTCACG2598881.4512759756330738No Hit
TCCGACACAATT2135841.1927035029690267No Hit
ATGTCGAGAGAA2126251.187348220460284No Hit
GAACTAGTCACC1969571.0998544077939856No Hit
TCCAAAGTGTTC1967361.0986202915954117No Hit
ATTGGGCTAGGC1946331.0868766428822878No Hit
CACGCCATAATG1908781.0659078359789211No Hit
GCGATATATCGC1901541.061864848975449No Hit
ATTCCTGTGAGT1874211.0466031314609614No Hit
AAGGAGCGCCTT1835141.024785520656313No Hit
CACGGTTGTGAG1813331.0126063015201632No Hit
CCAGTGTATGCA1789530.9993158193816777No Hit
GGAGACAAGGGA1741780.9726510915618171No Hit
GAATGATGAGTG1588200.8868883921152373No Hit
GAGTGGTAGAGA1532980.8560522398594738No Hit
CTAACCTCCGCT1508900.8426053991075945No Hit
CAGCGGTGACAT1476630.824585068913942No Hit
ACGTGTACCCAA1430020.7985569440200424No Hit
TGACCTCCAAGA1354540.7564071292379886No Hit
TATCGTTGACCA1346040.7516605284742437No Hit
GACAGGAGATAG1341080.7488907473226938No Hit
ACACGTAAGCCT1301280.7266654872759828No Hit
CGGGTTTGACGA1281580.7156645419764801No Hit
GCACACACGTTA1272880.7108062564888824No Hit
CGCCAAATAACC1237110.6908314436278058No Hit
ACACTAGATCCG1174710.6559858097857262No Hit
GTCTAATTCCGA1142510.6380045692454223No Hit
GCTGATGAGCTG1104090.6165499337932957No Hit
AACACAAGGAGT1082520.6045047363257692No Hit
GACGGAACCCAT1076260.6010090044691759No Hit
ACTCACGGTATG1065830.5951846461202513No Hit
CAGGCGTATTGG1059500.5916498246103097No Hit
GTACATACCGGT1007220.5624554377951827No Hit
GAATCTTCGAGC1003770.5605288763087216No Hit
TCTGTTGCTCTC963320.5379406409094889No Hit
ACACCTGGTGAT943090.5266437310917762No Hit
CGGACTACAACT866750.4840136719971552No Hit
ATCGATCTGTGG865890.48353342768458807No Hit
CGAGAAGAGAAC861550.48110986917698184No Hit
TACACGATCTAC845100.47192380064008754No Hit
AACCGCGGTCAA836970.4673838166154704No Hit
CCATGCGATAAC833840.4656359506871738No Hit
GACTTTCCCTCG828480.46264280008791825No Hit
TAACACCACATC820010.45791295203275134No Hit
ATGTGCACGACT814660.45492538566968843No Hit
TACAGATGGCTC795080.4439914512044974No Hit
TCACGGGAGTTG788780.4404733824031336No Hit
AATCCGTACAGC777420.4341296900882935No Hit
ACAAGGAGGTGA744410.41569612641638565No Hit
TAGGATTGCTCG743230.4150371865456541No Hit
CTCGAGAGTACG738600.4124516851884612No Hit
CTTACACCAAGT718840.4014172344718027No Hit
AAGAGATGTCGA670600.37447887907850275No Hit
GCATGGCTCTAA635520.35488937851471825No Hit
GTATTACGATCC633880.35397356377912514No Hit
TTCCTAGGTGAG622160.3474288389613499No Hit
TTGCGCATACTA611890.3416938283915077No Hit
TCCTCTGTCGAC607320.3391418324514707No Hit
GAGGAATAGCAG600710.3354506523281351No Hit
CGCAGCGGTATA586450.3274875315174291No Hit
GGATCGCAGATC572270.3195690845962642No Hit
ATGAGACTCCAC562180.31393459027788945No Hit
ATCCCGAATTTG555580.31024899439074644No Hit
GAACCAAAGGAT554630.30971849195244555No Hit
CTCTACCTCTAC550740.30754622407350823No Hit
TAGGAACTGGCC545730.3047485217409951No Hit
AGTAGAGGGATG532680.29746109350959865No Hit
CAGTGCATATGC531220.29664579502547306No Hit
GTCAATTGACCG515790.28802931858022807No Hit
CAAGCATGCCTA514750.2874485580161934No Hit
AGTTCCCGAGTA513220.28659416987871933No Hit
TCTTCCGCTACT512720.28631495806908724No Hit
GACTTGGTATTC511880.28584588222890545No Hit
AGAGCCTACGTT509800.2846843611008361No Hit
AGCCTAAGCACG499710.2790498667824614No Hit
GAAGTTGGAAGT473330.26431865170627455No Hit
CCTCGTTCGACT473150.264218135454807No Hit
TCGGAGTGTTGT473100.2641902142738438No Hit
GATTGGTTGCAC472640.2639333394089823No Hit
CTATTTGCGACA456820.2550990777522243No Hit
GTACCTAATTGC451840.25231812812828913No Hit
TAGCTCGTAACT444400.24816345640096424No Hit
TCCCAGAACAAC429820.24002164003209372No Hit
TCGAGGACTGCA426030.23790521451508279No Hit
AGCCGGCACATA422570.23597306879242905No Hit
TGGCACCGATTA414400.23141074782304139No Hit
CTAGCGAACATC398620.22259882311105395No Hit
TAAACCGCGTGT396010.22114133746477463No Hit
CGACTGTCTTAA388720.21707042928033937No Hit
TCAACAGCATCG384120.21450168063172453No Hit
CAGGAAGGTTAA384120.21450168063172453No Hit
TGGCAAGACTCT371200.20728684747083242No Hit
GAAGAAGCGGTA370320.20679543468588002No Hit
GTTGGTCAATCT368010.20550547612537992No Hit
ACGCGCAGATAC359540.20077562807021307No Hit
AGCTGGAAGTCC347910.1942811613781716No Hit
TGTTATCGCACA345770.19308613483294645No Hit
AAGATGGATCAG345680.19303587670721267No Hit
TCAAGCTCAAGC328680.18354267517972306No Hit
AGGCATCTTACG328620.1835091697625672No Hit
CTTCGGCAGAAT323690.18075614131959522No Hit
CTACCGGATCAA322510.1800972014488636No Hit
CGTGACAATGTC313300.17495411991544127No Hit
TTATGCAGTCGT305880.17081061666050168No Hit
AGATGTTCTGCT305220.17044205707178736No Hit
GACTCGAATCGT294790.16461769872286286No Hit
CCTCTCGTGATC293770.16404810663121347No Hit
GCCTGAATTTAC293590.16394759037974593No Hit
ATGTGGGACCCA284910.15910047336453356No Hit
GGTCACTGACAG276260.15427010905789915No Hit
TGCAATGTTGCT272480.15215926777708086No Hit
GCAGGATAGATA252840.141191827894734No Hit
CAGCTAGAACGC250210.13972317377606944No Hit
GGAAACCACCAC246610.1377128487467187No Hit
ATCGCTCGAGGA233550.1304198362791296No Hit
CGATCCGTATTA221890.12390861687851026No Hit
AATGTCCGTGAC214880.11999406730746894No Hit
CGGAGCTATGGT214400.11972602397022217No Hit
GTGGGATGTTTC199410.11135525391745336No Hit
TTAGGGCTCGTA198010.11057346085048363No Hit
ACCACATACATC194200.10844586686108743No Hit
AATGCCTCAACT192220.10734018809494453No Hit
ACGGGACATGCT187920.10493896653210892No Hit
GTACGTGGGATC187410.10465417048628421No Hit
TAATCCACAGCG180040.10053858841230782No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGAT650.00125148686.0041861
AGCAAAC1619500.06.00270371
CTGCTAA420900.06.0020461
ATATCCG3750.05.99917366
GTCAGCC1800.05.9991575
TTAAGCC650.00126413415.9991575
GGGCCAG1850.05.9991574
AACAGGC704.7060114E-45.9991575
GCCCCGG1001.2899218E-65.99915654
GTAATCC953.438241E-65.99915655
GGGCGCC1451.9645086E-105.99915654
GGGGCGC1451.9645086E-105.99915653
TATGCAC887300.05.99674271
CCTGAAC285550.05.99577571
ATTGGGC209350.05.9955821
ACGTGTA155700.05.9945451
ATGCACC872800.05.99367482
GCAAACA1635650.05.9916552
TGCACCA865950.05.9915363
CGTGTAC156500.05.9915072
Produced by FastQC (version 0.11.2)