FastQCFastQC Report
Thu 3 Aug 2017
000000000-B4HVB_l01n01.33100000098ee5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4HVB_l01n01.33100000098ee5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17907552
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTCGGTCCAT14011827.824531236877045No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGCGGGCTGCCTCCGGGC9657985.393244146380254No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGACCCCTCGCGGGT9486685.297586180400314No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTATTGCCTGCGCTTA6611073.691777636608287No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTTCTAACGAGCC4004622.236274394177384No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGGTCC3355581.873835128330215No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTTGAACGGGTTGTAGC2745821.53333074224774No Hit
GTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCTGGA2714811.5160140258143602No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATAACTATGGTGTTGGTTG2626021.4664315926599012No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAGAAACAAAAAGCTGTCTCGTG2247421.2550124104065146No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTAATTATTGAGTGTAAAA2194901.2256840019227642No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTT1971981.1012002087164119No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCC1667080.9309368472027891No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTTGTAGACTTCGGTC1658890.9263633577610162No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAGAACCTGGGCCTGCGCTACTT1527510.8529976626620993No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCTGCCTCCGGGC1525000.8515960193777464No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCATAGG1229290.6864645709251606No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTACGGCCT1105440.6173038056793022No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAA1098210.6132664029120228No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGTACACGTCAAGTGTTCTTTA1050800.5867915391227121No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACCACA972540.5430893066791039No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTTGACCCCGGCCCTCGGGC874920.4885759929665428No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATTGCTCTTTGAGCGTTAA867660.4845218374906855No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAACATTTAGGCCGTAACAAGCCT805360.4497320460105323No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCAATATGAAAGCGAATGGGGAA782610.43702790867227415No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTCTTTGAACGGGTTGTAG782180.43678778651599054No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAGTTTTGAAAGGGTTGTAGC744740.4158804062107428No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAGTTTTGAAAGGGGTTGTAG722350.4033773013754198No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGATTAACTGTCTGTCGAGCTT696800.38910957790322204No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCGTGGCCC690600.38564735146378465No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTTGACTGGGTTGTAGC683900.38190591321471523No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGTAAACAAAAAGGCCTTGGGT666000.3719101304298879No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAATCAAACAAGTTCATCTTG660730.3689672379563661No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCCTCGTGGCC637360.3559168779741642No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATGAAATTGAGTGGGGTTG622930.34785882514818334No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATTTAGAGCGTAACTTCGG608450.3397728511412392No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAGTTTTGAAATGGGTTGTAG600560.33536688878524545No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTCTTGACTGGGTTGTAGC559300.31232633025440887No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGCGGGCCCTCTGGGTCC556000.31048353231083736No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACAAAGTTCGCTTCTCTGAAGCA538290.3005938500136702No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAAATAGGAGAAAGGGCGCTT533970.2981814599784493No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGATTGAACGTCTGTCGAGCTT527000.29428924735217854No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTCCCTCGTGGCC496860.2774583594675587No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTTCCAACGAGCC488230.27263916363330953No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAATTATGTTTTCTAGATGGG480140.26812151655346306No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCACATATCATCGGGGGGGAAAC469140.26197885674155796No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAGAATATTGGACTTCGGTCCAT457900.2557021752610295No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGGAACGCGGCCGGGCCC449120.25079921588389076No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAAACATTGTGAAGGAGGTTG435410.24314322806378003No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTTTTAGGGCGCTCTTTT434550.24266298375121292No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGTTTGGATGCCCCTTCT420840.23500699593110214No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTGCGGACCCCTCGCGGGT382100.21337366492081106No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTTATTAGTTCCAGGCCAGCG372920.20824733609596668No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGGAAATTCCGGCCGAGGGG356790.1992399631172368No Hit
GTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTCGGCCCAT336590.18795980600810205No Hit
GTTGGTGAACCAGCGGAGGGATCATTACTGAGTTTTCTAAACTCCCAAAC332880.18588805438063227No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGATAGGGCCTCCGGGCTCG326030.18206285258867322No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAAATAGGAGAAGGTTGCTTA320190.1788016586521709No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTAATTATTGAGTGATAAA301660.1684540689872072No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCCGGCTCCGGCCG278710.1556382469250962No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGGTGCGGGCTGCCTCCG277570.1550016439991351No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGCGAAGTTCGGAATGCGTGTCCG276130.1541975139873948No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAGTTTTGAAACGGGTTGTAG265610.14832289751273653No Hit
GTAGGTGAACCTGCGGAAGGATCATTATAGTATTCTATTGCCTGCGCTTA263740.14727864534471266No Hit
GTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTCGGTCCAC244250.13639496900525544No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGCGGACCCCTCGCGGGT238790.13334597604407347No Hit
GTAGGTGAACCTGCGGAAGGATCACTAAAGTAAACGCCCTCCGGGGCTCT231910.1295040215435365No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAACATAGGACGTCCAACTTA224790.12552804537437612No Hit
GTAGGTGATCCTGCGGAAGGATCAGTAGAGAATATTGGACTTCGGTCCAT212320.11856450284215285No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGATTGAATTATCATTGTTGCTCG211900.11832996492206194No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCC205070.11451593160248816No Hit
GTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGGCTTCGGTCCAT203500.11363920652024352No Hit
GTAGGTGAACCTGCGGAAGGATCAGTAGTGAATATTGGACTTCGGTCCAT202750.11322038880579545No Hit
GTAGGTGGACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAA200630.11203653073295558No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGATTCGGGCTTCGGCCTAACC195400.10911597520420434No Hit
GTAGGTGAACCTGCGGGAGGATCAGTAGAGAATATTGGACTTCGGTCCAT193610.10811639692572161No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGATTGACCGTCTGTCGAGCTT187710.10482169757206346No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGCGGGCTGCCTTCGGGC187380.10463741777770628No Hit
GTTGGTGAACCAGCGGAGGGATCATTACAGAGTTATCCAACTCCCAAACC184950.10328044838289455No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAATTTCTCAAAGTTGCTTTT184170.10284487795986855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTGA15978250.0244.385672
GGTGAAC16259200.0244.195624
GTAGGTG16160000.0243.883211
AGGTGAA16006150.0243.778273
TGAACCT16271450.0243.693316
GAACCTG16423850.0241.790797
ACCTGCG17229750.0231.641639
AACCTGC17144150.0231.500638
GTGAACC17621400.0226.860265
ACCAGCG203700.0205.068319
TCGATGG748500.0202.20288245
GAACCAG210000.0197.924597
GGACCTG149700.0195.819567
AGGTGGA147400.0195.72033
TTGGTGA233750.0195.42642
GACCTGC151750.0193.497138
CGATCTT215900.0192.06244245
GTGGAGT122600.0190.5446245
AACCAGC220300.0189.671658
TGGTGAA241300.0188.956383
Produced by FastQC (version 0.11.2)