FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_zymobiomics_community_standard.3520000008bde8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_zymobiomics_community_standard.3520000008bde8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633208
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC1600.002174275722.6562483
TAGACAC2205.9000833E-419.7727265
TATACGG3201.9261168E-518.1249985
GTAATAC5251.036824E-917.9523813
GGTACAC2050.00912281417.6829263
AGCACTC2050.00912281417.6829268
GTACTGA3352.8555432E-517.3134336
GTATAAC3352.8555432E-517.3134331
GGATATA4307.5919706E-716.8604661
GACTGAC3153.5035482E-416.111117
TAAGCAG3706.688529E-515.6756745
TTAGGGT2800.002969803315.5357144
TATATAC5203.259247E-715.3365383
GATATAC3355.594918E-415.1492542
TCTGTAC3406.2606606E-414.926473
TACGACC3456.993512E-414.7101444
TGGAGAG3000.00469951114.5000015
GTACTGG5556.8129884E-714.36936951
TACGACT3558.683149E-414.2957744
GTCTTAT3558.683149E-414.2957741
Produced by FastQC (version 0.11.2)