FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_zika-la-100000.3520000008bda5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_zika-la-100000.3520000008bda5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113451
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG22451.978827864011776No Hit
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC20971.8483750694132268No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG17051.5028514512873399No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG16581.4614238746242871No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG15931.4041304175370866No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC15191.338904020237812No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA15131.3336153934297628No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG14991.3212752642109808No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC14851.3089351349921994No Hit
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC13611.1996368476258472No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC12971.1432248283399882No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC12901.1370547637305974No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG12221.077116993239372No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA12201.075354117636689No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA11701.0312822275696116No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA11651.0268750385629037No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG11461.0101277203374144No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG10760.9484270742435061No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG10550.9299168804153334No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG10420.9184581889978933No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC10420.9184581889978933No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC10330.9105252487858194No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG10230.901710870772404No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC9970.8787934879375237No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG9970.8787934879375237No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG9960.8779120501361821No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC9730.8576389807053265No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC9620.8479431648905695No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG9550.8417731002811787No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT9310.8206185930489815No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC9240.8144485284395906No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC9140.8056341504261753No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC8710.7677323249684886No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC8500.749222131140316No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG8310.7324748129148266No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG8250.7271861861067773No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC7960.7016244898678725No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG7670.6760627936289676No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC7630.6725370424236014No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG7510.6619597888075028No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG7330.6460939083833549No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT7170.6319909035618901No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG7130.6284651523565239No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG7070.6231765255484747No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG7040.6205322121444501No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC7010.6178878987404254No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG6960.6134807097337176No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG6720.5923262025015205No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG6690.5896818890974959No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC6570.5791046354813972No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA6500.5729345708720064No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC6440.5676459440639571No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC6360.5605944416532248No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG6280.5535429392424924No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG6250.5508986258384677No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG6250.5508986258384677No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG6230.5491357502357846No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT6140.5412028100237107No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC6120.5394399344210276No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT5810.5121153625794396No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG5790.5103524869767565No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG5640.4971309199566332No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG5560.49007941754590084No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC5350.47156922371772836No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT5100.4495332786841897No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA4910.43278596045870027No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG4830.4257344580479679No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA4750.4186829556372355No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT4730.4169200800345524No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT4710.41515720443186926No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC4630.4081057020211369No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC4510.3975284484050383No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT4380.38606975698759816No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC4240.37372962776881646No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT4190.3693224387621088No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG4170.3675595631594257No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA4170.3675595631594257No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC4140.364915249755401No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC4050.3569823095433271No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC4010.3534565583379609No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC3990.3516936827352778No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT3980.35081224493393626No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC3900.3437607425232038No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG3850.3393535535164961No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT3770.3323020511057637No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC3770.3323020511057637No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG3630.3199619218869821No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT3550.31291041947624965No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT3540.3120289816749081No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT3510.30938466827088346No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT3510.30938466827088346No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC3450.3040960414628342No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT3410.30057029025746795No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA3250.2864672854360032No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC3170.2794157830252708No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT3170.2794157830252708No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC3080.2714828428131969No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC2960.26090558919709833No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG2930.2582612757930737No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC2920.2573798379917321No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC2800.24680258437563352No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG2680.23622533075953497No Hit
CGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA2670.2353438929581934No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTG2630.23181814175282722No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC2620.2309367039514857No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTT2530.22300376373941172No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA2500.2203594503353871No Hit
CAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTT2390.21066363452063006No Hit
CAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT2390.21066363452063006No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC2390.21066363452063006No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC2300.20273069430855611No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACA2290.20184925650721455No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC2280.200967818705873No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTG2260.1992049431031899No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG2260.1992049431031899No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACA2260.1992049431031899No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG2230.19656062969916527No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG2140.18862768948709133No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGAC2110.1859833760830667No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC2060.18157618707635897No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC1910.16835462005623572No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC1890.16659174445355263No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG1890.16659174445355263No Hit
CGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA1800.1586588042414787No Hit
CAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTT1780.15689592863879562No Hit
CAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTT1750.15425161523477096No Hit
CGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACA1690.14896298842672168No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC1690.14896298842672168No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGAC1670.14720011282403855No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTG1610.14191148601598927No Hit
ACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGT1530.1348599836052569No Hit
CAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTT1400.12340129218781676No Hit
CGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACA1370.12075697878379212No Hit
CAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTT1370.12075697878379212No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTG1360.11987554098245057No Hit
ACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGT1360.11987554098245057No Hit
CTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAA1350.11899410318110903No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTG1210.10665397396232734No Hit
GTCACAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC1200.1057725361609858No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGT100.007082637145.0145
GCAGAAG100.007082637145.0145
TGACTGC555.2041087E-621.09091140-144
CCAGACT2155.018252E-420.232564
AGTCCAG2205.859226E-419.7727281
TGTCGCC3301.1198808E-619.772728145
GTTCTGA601.10414385E-519.333332135-139
TCCAGAC2256.816441E-419.3333323
GTCCAGA2256.816441E-419.3333322
TCTCAGT400.00791196418.125135-139
TTCTGAC652.200244E-517.846153135-139
GACTGTC3554.6403893E-516.3380286
TCTGACT654.443207E-415.615384135-139
CTGACTG600.004698713314.5140-144
TACTGCG801.2997097E-414.5140-144
CGTACTG800.002135160812.6875135-139
AGACTGC181300.011.0369555
CAGACTG183150.010.9254714
TCAGACT182850.010.9037473
TTCAGAC185100.010.8103722
Produced by FastQC (version 0.11.2)