FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_zika-la-10000.3520000008bd98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_zika-la-10000.3520000008bd98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18028
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC2441.3534501885955181No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG2371.3146216995784337No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA2071.148213889505214No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG1901.053916130463723No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA1800.9984468604393165No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG1770.9818060794319947No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC1730.9596183714222322No Hit
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC1730.9596183714222322No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG1660.9207898824051476No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC1650.915242955402707No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC1650.915242955402707No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA1600.8875083203905035No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC1590.881961393388063No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG1550.8597736853783005No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG1540.8542267583758598No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC1500.832039050366097No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC1500.832039050366097No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC1490.8264921233636565No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC1490.8264921233636565No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC1480.8209451963612159No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG1470.8153982693587752No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA1390.77102285333925No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC1340.7432882183270468No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC1300.7211005103172842No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG1220.6767250942977591No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG1190.6600843132904372No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC1170.6489904592855559No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG1150.6378966052806745No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC1130.6268027512757932No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC1120.6212558242733526No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG1110.6157088972709118No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT1100.6101619702684713No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT1100.6101619702684713No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG1090.6046150432660307No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC1070.5935211892611494No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG1070.5935211892611494No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC1050.582427335256268No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG1040.5768804082538274No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG1020.5657865542489461No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG1020.5657865542489461No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG1010.5602396272465054No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT980.5435988462391835No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG970.5380519192367429No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT970.5380519192367429No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG960.5325049922343023No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC950.5269580652318615No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG920.5103172842245396No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG910.504770357222099No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG900.49922343021965826No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC870.4825826492123364No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG870.4825826492123364No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT850.471488795207455No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG830.4603949412025738No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG820.45484801420013315No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT820.45484801420013315No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG820.45484801420013315No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC810.4493010871976925No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG810.4493010871976925No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT800.44375416019525177No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC800.44375416019525177No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT780.43266030619037055No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG780.43266030619037055No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG770.4271133791879299No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT770.4271133791879299No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC750.4160195251830485No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC710.393831817173286No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA690.3827379631684047No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT680.37719103616596406No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG680.37719103616596406No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT660.3660971821610827No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC650.3605502551586421No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG630.3494564011537608No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC630.3494564011537608No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG630.3494564011537608No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC620.34390947415132017No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT610.33836254714887953No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC610.33836254714887953No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG600.33281562014643884No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT590.32726869314399826No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC590.32726869314399826No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA580.32172176614155756No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC560.3106279121366763No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT560.3106279121366763No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC560.3106279121366763No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT540.299534058131795No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT530.2939871311293543No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC520.2884402041269137No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC510.28289327712447304No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA500.2773463501220324No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC490.27179942311959177No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC480.2662524961171511No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC470.26070556911471043No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC460.2551586421122698No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG440.24406478810738852No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC430.23851786110494788No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA410.22742400710006658No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACA380.21078322609274464No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG370.20523629909030397No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA360.19968937208786333No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC350.19414244508542267No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC340.18859551808298203No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC340.18859551808298203No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG340.18859551808298203No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG340.18859551808298203No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT330.18304859108054136No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG330.18304859108054136No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACA330.18304859108054136No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA320.17750166407810075No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC310.17195473707566009No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG310.17195473707566009No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC310.17195473707566009No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTG300.16640781007321942No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG300.16640781007321942No Hit
CAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTT300.16640781007321942No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC290.16086088307077878No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC270.1497670290658975No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTG270.1497670290658975No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG270.1497670290658975No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTG260.14422010206345684No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTT260.14422010206345684No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGAC250.1386731750610162No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG250.1386731750610162No Hit
ACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGT240.13312624805857556No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGAC230.1275793210561349No Hit
CAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT230.1275793210561349No Hit
CAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTT220.12203239405369426No Hit
CGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA220.12203239405369426No Hit
CAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTT220.12203239405369426No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTG210.1164854670512536No Hit
CGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA210.1164854670512536No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC210.1164854670512536No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTG200.11093854004881294No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTG200.11093854004881294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2456.0881575E-910.061224120-124
GTTCAGA24950.09.5891781
TTCAGAC25450.09.4007862
AGACTGC25500.09.3823535
TCAGACT25700.09.0272383
CAGACTG25900.08.6776074
CTGCCGC2450.00381837886.510204140-144
GACTGCG55050.05.66303356
ACTGCGA53850.05.5199637
TGTCGCA67950.05.44150079
TGCGACA53750.05.39534859
CTGTCGC70950.05.21141628
TGAACTG30651.7375824E-65.2039154
ACTGTCG69850.05.18969257
GTCTGAA31102.2975564E-65.12861731
CTGCGAC55500.05.09459458
Produced by FastQC (version 0.11.2)