FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_zika-la-1000.3520000008bd8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_zika-la-1000.3520000008bd8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95911
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC22262.3209016692558726No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG19612.0446038514873166No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG17981.8746546277277893No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC17341.8079260981534964No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG15801.6473605738653543No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG11911.2417762300466058No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC11821.2323925305752208No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG10871.1333423694883797No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC10711.1166602370948067No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG10601.105191271074225No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC9981.040548008049129No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA9911.0332495751269406No Hit
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC9881.0301216753031457No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA9631.0040558434381877No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC9420.9821605446716227No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT9180.9571373460812629No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG8880.9258583478433131No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG8090.8434903191500454No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG7960.8299360865802672No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC7830.8163818540104889No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC7730.8059555212645055No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA7650.7976144550677191No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG7510.7830175892233424No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG7390.7705059899281625No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA7330.7642501902805726No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG7250.7559091240837861No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC7090.7392269916902128No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC7030.7329711920426228No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC7000.7298432922188278No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC6980.7277580256696312No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG6900.7194169594728447No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG6720.7006495605300748No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC6600.6881379612348948No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC6410.6683279290175267No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC6320.6589442295461417No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC6280.6547736964477484No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG6130.6391341973287735No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG5840.608897832365422No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT5760.6005567661686355No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC5720.5963862330702422No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC5590.5828320005004639No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC5540.5776188341274724No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG5500.5734483010290791No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC5420.5651072348322924No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG5280.5505103689879159No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG5210.5432119360657276No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG5170.5390414029673343No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG5120.5338282365943426No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG4950.5161034709261711No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG4880.5088050380039829No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG4880.5088050380039829No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG4850.5056771381801879No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC4810.5015066050817946No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA4780.4983787052579996No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG4670.48690973923741804No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG4600.4796113063152297No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG4510.47022760684384485No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT4200.4379059753312967No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG4080.4253943760361168No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT4000.4170533098393302No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG3980.4149680432901336No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC3950.41184014346633857No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT3940.4107975101917402No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG3620.37743324540459383No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG3610.3763906121299955No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT3560.3711774457570039No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG3550.3701348124824056No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT3530.36804954593320893No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT3360.3503247802650374No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA3300.3440689806174474No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT3220.33572791442066086No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC3210.3346852811460625No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC3200.3336426478714642No Hit
CGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA3190.33260001459686583No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA3130.3263442149492759No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA3060.3190457820270876No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC3020.31487524892869434No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT2980.310704715830301No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG2960.30861944928110435No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG2940.3065341827319077No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC2930.3054915494573094No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC2880.30027838308431776No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG2850.29715048326052274No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT2790.2908946836129328No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT2770.2888094170637362No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC2710.2825536174161462No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC2700.2815109841415479No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT2680.27942571759235124No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC2660.2773404510431546No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC2650.2762978177685563No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC2650.2762978177685563No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA2590.2700420181209663No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT2530.26378621847337635No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT2490.25961568537498303No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT2310.2408482864322132No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT2120.22103825421484502No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC2110.21999562094024672No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC2100.21895298766564836No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG2080.21686772111645172No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC2010.20956928819426346No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTG1900.19810032217368184No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTG1900.19810032217368184No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG1890.19705768889908354No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC1870.19497242234988688No Hit
CAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT1870.19497242234988688No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC1860.19392978907528854No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGAC1820.18975925597689525No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACA1800.18767398942769858No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC1780.18558872287850195No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG1770.18454608960390362No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC1690.17620502340711702No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG1660.17307712358332206No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTT1660.17307712358332206No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG1620.16890659048492873No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC1560.1626507908373388No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG1550.16160815756274047No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC1530.1595228910135438No Hit
CGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA1510.15743762446434717No Hit
CCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAA1450.1511818248167572No Hit
CAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTT1420.14805392499296222No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACA1420.14805392499296222No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA1400.14596865844376558No Hit
CGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACA1350.14075549207077395No Hit
ACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGT1350.14075549207077395No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC1310.13658495897238063No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC1290.134499692423184No Hit
CAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTT1240.12928652605019236No Hit
GGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACT1230.12824389277559403No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGAC1210.1261586262263974No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC1200.12511599295179907No Hit
ACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGT1170.1219880931280041No Hit
CAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTT1170.1219880931280041No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC1140.11886019330420912No Hit
GTCACAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC1120.11677492675501247No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTG1110.11573229348041414No Hit
CAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTT1060.11051912710742251No Hit
CAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTT1010.10530596073443088No Hit
CTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAA1000.10426332745983255No Hit
CGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACA990.10322069418523423No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTG960.10009279436143925No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTA100.0070809405145.0145
TTCCATT300.001930391272.54
TCCATTA350.003556681362.1428575
GTCGTAG1204.0310167E-430.208334145
TAACTGT304.3939912E-529.000002140-144
GGACAGT200.006071347329.0140-144
GTACTGC351.2543934E-424.857143140-144
TCGTACT300.00149314124.166668135-139
TCCAGAC1650.002583911621.9696962
GTCCAGA1650.002583911621.9696961
GACAGTC2704.3760283E-621.4814823
GTTCCGA350.003655993120.714287135-139
CTGTACT400.00791022718.125135-139
TACTGCG500.001391962517.4140-144
ACAGTCC6505.638867E-1116.7307684
CCGACTG600.004697304714.500001140-144
GACTGTC4302.3688376E-413.4883736
CACAGTC5458.72097E-613.30275253
TCACAGT5509.582438E-613.1818172
GTCACAG5801.655368E-512.51
Produced by FastQC (version 0.11.2)