FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_zika-la-100.3520000008bd7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_zika-la-100.3520000008bd7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114067
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG10690.9371685062287953No Hit
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC10520.9222649846143056No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC8680.760956280081005No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG8480.7434227252404288No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG8360.7329025923360831No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG8160.7153690374955071No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA7990.7004655158810172No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG7210.6320846520027703No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA7110.6233178745824822No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC6970.6110443861940789No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG6910.605784319741906No Hit
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC6460.5663338213506097No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC6030.5286366784433709No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA5990.5251299674752558No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC5930.519869901023083No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC5810.5093497681187372No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA5660.49619960198830515No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC5590.4900628577941035No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC5530.4848027913419306No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG5370.4707759474694697No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG5190.4549957481129512No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG5100.44710564843469186No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC5090.44622897069266304No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG4920.4313254490781734No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC4890.4286954158520869No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG4870.42694206036802934No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC4840.4243120271419429No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC4770.41817528294774126No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC4610.4041484390752803No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT4500.39450498391296346No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC4450.3901215952028194No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC4250.37258804036224324No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG4230.37083468487818566No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG4150.36382126294195516No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG4120.36119122971586876No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG4110.36031455197383994No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG4020.35242445229558067No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC3810.3340142197129757No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG3790.3322608642289181No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG3770.3305075087448605No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG3730.3270007977767453No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG3650.3199873758405148No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG3440.30157714325790985No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC3420.2998237877738522No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT3390.2971937545477658No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC3390.2971937545477658No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG3260.2857969439013913No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG3240.2840435884173337No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG3150.2761534887390744No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG3120.273523455512988No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG3090.27089342228690155No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC3090.27089342228690155No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG3080.2700167445448728No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC3020.26475667809269987No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC3020.26475667809269987No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG3010.2638800003506711No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT3000.2630033226086423No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT2990.26212664486661347No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA2970.2603732893825559No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG2900.25423654518835426No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG2820.24722312325212376No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG2810.24634644551009494No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT2760.24196305679995092No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC2660.2331962793796628No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT2660.2331962793796628No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG2620.22968956841154758No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG2560.22442950195937475No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT2500.21916943550720192No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA2470.2165394022811155No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC2450.21478604679705787No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT2440.21390936905502905No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT2430.21303269131300026No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT2400.2104026580869138No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT2220.1946224587303953No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA2150.18848571453619364No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT2120.1858556813101072No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC2090.18322564808402078No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT2080.182348970341992No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC2080.182348970341992No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA2050.17971893711590556No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC2050.17971893711590556No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC1940.17007548195358868No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG1900.16656877098547346No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC1870.16393873775938703No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG1860.16306206001735823No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG1820.15955534904924298No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA1790.15692531582315658No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT1740.15254192711301254No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC1720.15078857162895493No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT1700.14903521614489731No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC1680.14728186066083968No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC1660.14552850517678206No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT1650.14465182743475327No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC1640.14377514969272445No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG1630.14289847195069566No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT1620.14202179420866684No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC1600.1402684387246092No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG1580.1385150832405516No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC1520.13325501678837876No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC1520.13325501678837876No Hit
CGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA1460.12799495033620592No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC1410.12361156162606188No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC1390.12185820614200427No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG1380.12098152839997546No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA1370.12010485065794664No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACA1370.12010485065794664No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC1280.11221475097968737No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC1260.11046139549562976No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC1220.10695468452751453No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC1210.10607800678548572No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG1200.1052013290434569No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC1190.10432465130142811No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC1160.10169461807534169No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGGT100.0070826868145.07
AGAAGTA100.0070826868145.0145
GCCGGTC100.0070826868145.08
GCCTAAA203.8571848E-4108.751
TAAGACA406.6379274E-572.54
TTTAAGA350.003558409662.1428532
CTAAGGC350.003558409662.1428533
ATTTAAG400.006037204554.3751
CCGTGGG400.006037204554.375145
TTCCGGC400.006037204554.3752
TAAAAGT400.006037204554.3754
CCTAAGG400.006037204554.3752
AATGCTA400.006037204554.3759
CAGATGC400.006037204554.3758
GTATATA400.006037204554.3751
CAATGCT400.006037204554.3758
GGTTCTG400.006037204554.3757
TAATATA450.00961742248.3333322
TAACACT450.00961742248.3333326
GTCCAAA604.926551E-448.3333321
Produced by FastQC (version 0.11.2)