FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_zika-la-10.3520000008bd61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_zika-la-10.3520000008bd61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308664
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC6010.19471010548687245No Hit
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA5210.16879195500609076No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC4120.1334784749760257No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT4030.13056268304693777No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT4030.13056268304693777No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC3700.11987144597361532No Hit
CTATTAGGGTCAGGGGTGTTAATTGTGAGTAGCAACCTATCATTAGCAGC3270.10594044009019517No Hit
GTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTG3270.10594044009019517No Hit
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC3250.10529248632817562No Hit
CCCTAATAGTGGCCATCATTTTGCTCGTGGCGCACTACATGTACTTGATC3240.10496850944716585No Hit
CAGCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTG3140.10172874063706815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTA100.007088505144.999984
ACAGTTC300.0019344772.53
TCGAGTT451.1925561E-464.444443
GTATTAT954.5474735E-1061.0526281
TTATGAC1051.1041266E-955.23814
CGAGTTT553.2155102E-452.7272764
TCTTCCG1650.052.7272725
ATTATGA1152.4738256E-950.4347843
CTTCCGT1900.049.605266
AAAACCT450.00963287348.3333325
TTCCGTG2000.047.1257
GTACCTC1109.9293175E-846.1363681
TTCGAGT657.328671E-444.6153872
ATAATAC1002.7279712E-643.53
TTAGACT1451.920489E-840.04
GTCATAA750.001481796438.6666641
GTTCGAG750.001481796438.6666641
TCATAAT951.02724E-438.157891
TGTACCG1553.4569894E-837.4193535
TATATTA2850.035.6140372
Produced by FastQC (version 0.11.2)