FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_zika-her-reg.3520000008bdb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_zika-her-reg.3520000008bdb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360419
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA30930.8581678546358544No Hit
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC29330.8137750784503591No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC25550.7088971447121267No Hit
GTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTG24120.6692211009963404No Hit
GTCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT22640.6281577830247573No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC20890.5796031840718718No Hit
GTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC19360.5371525918444922No Hit
CCCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA19240.53382313363058No Hit
GTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT15720.4361590260224905No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAA14910.41368518307858354No Hit
TCCCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTG13820.38344260430221494No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGG12070.3348880053493295No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC11820.3279516340703459No Hit
TACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGT11750.3260094501122305No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC10780.299096329549774No Hit
TCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTC10580.2935472325265871No Hit
GTGTTGGGCAGCGGCTGTCAGAAGCCATGTCTGATATTGATGCCTCATAG10560.29299232282426846No Hit
GCTTCCTGGTGGAATGGGAGGGGAGCGTCACAGCTCTTCTAGATCTCCGT10260.2846686772894881No Hit
CTCAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGA9690.2688537507734054No Hit
GTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGATTGCTTGTCAA9220.2558133727689162No Hit
AGTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCT9000.2497093660434106No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG8890.2466573626806578No Hit
CCCACACCTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA8680.24083081080631152No Hit
CAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAAT8530.23666898803892136No Hit
ATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCCCC8520.23639153318776202No Hit
CATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGG8520.23639153318776202No Hit
TGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGC8500.23583662348544335No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC8450.2344493492296466No Hit
GTGTATTCTCTTGATTCCAACCAGGTTTGCGACCGCGTTTGCAGCTTCCT8300.23028752646225642No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA8280.22973261675993772No Hit
GTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTTG7870.2183569678624046No Hit
ATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCA7820.2169696936066079No Hit
GCATAGGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACT7770.21558241935081113No Hit
GTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGA7400.20531658985791537No Hit
GTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTC7390.20503913500675602No Hit
TCCGTACACAACCCAAGTTGACGTCGTGTTGCACCAACAATCGACGTCAT7240.20087731223936584No Hit
GTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAG7230.2005998573882065No Hit
GAATAAGTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCA7220.20032240253704714No Hit
GAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGA6970.19338603125806353No Hit
GTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACT6910.19172130215110747No Hit
GTCAATATGCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTT6830.1895016633418327No Hit
GGGTATGGCTATTGGGTTCATGCCACAGATGGTCATCAGGACCACCTTGA6820.18922420849067334No Hit
CATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGG6810.188946753639514No Hit
ATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCAT6630.18395256631864582No Hit
TACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCC6580.18256529206284905No Hit
CCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGAT6520.180900562955893No Hit
TCTTTATTGTTTCCATAGCCTCTTTTTTCCCCACTGAACCCCATCTATTG6360.17646128533734348No Hit
GTGTACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCTAGAAG6360.17646128533734348No Hit
GGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAG6330.17562892078386547No Hit
ATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGA6260.17368673682575003No Hit
TCCTAGCATTGATTATTCTCAGCATGGCAGCCAGATCTTTCTTGAACTTC6230.172854372272272No Hit
CTATTGATGAGACCCAGTGATGGCTTGATTGCCGTGAATCTCAAAAAGGC6200.17202200771879395No Hit
GTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTGT6180.17146709801647528No Hit
GAATAATCAATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTAGT6130.17007982376067854No Hit
TCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTC6070.16841509465372248No Hit
GCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGC5910.16397581703517294No Hit
CTTCTAGATCTCCGTGCTTCACCTTTTTTGTGATGGCAGGTTCCGTACAC5820.16147872337473884No Hit
CACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGATTGCT5810.16120126852357952No Hit
ATGCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAG5620.15592962635155194No Hit
ATATCAGACATGGCTTCTGACAGCCGCTGCCCAACACAAGGTGAAGCCTA5470.15176780358416175No Hit
CAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAAAGA5390.149548164774887No Hit
CACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCCCCAGCATC5370.14899325507256833No Hit
AGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAG5330.14788343566793094No Hit
TCCCCAATGTGGTTGGAAAAGATATGGCCTCCCCAGCATCGTTTCTGTCC5290.14677361626329355No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC5280.14649616141213423No Hit
TCACACATGTGTCCAAGATCCATGATCTGTATATAACACTTATTCATCCC5150.1428892483470627No Hit
TAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCAG5110.14177942894242535No Hit
CTAGTATCTGCGCCTCGTCTCTTCTTCTCCTTCCTAGCATTGATTATTCT5070.14066960953778795No Hit
ATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACCAGGAAGCTGCAAA5060.14039215468662863No Hit
TCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTAT5040.13983724498430994No Hit
TCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAAAG4940.13706269647271646No Hit
TATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGGCA4880.1353979673657604No Hit
CTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTTTT4880.1353979673657604No Hit
ATCCAGCATAGGGCATTCATAGCTCATGGTGGCATCACACATGTGTCCAA4820.13373323825880434No Hit
CTAATCAAGTGCTTTGTGTATTCTCTTGATTCCAACCAGGTTTGCGACCG4800.13317832855648565No Hit
GATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACCAGGAAGCTGCAA4770.1323459640030076No Hit
CAATCAAGTCCTAGGCTTCCAAAACCCCCCAGGGTGGCTTCGGCTCTCGG4740.1315135994495296No Hit
GTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCAGCA4730.13123614459837024No Hit
GGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACACGACGT4690.13012632519373285No Hit
ATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTAT4680.1298488703425735No Hit
GGTTTGCGACCGCGTTTGCAGCTTCCTGGTGGAATGGGAGGGGAGCGTCA4670.12957141549141415No Hit
ATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGCAA4660.1292939606402548No Hit
GTACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCTAGAAGAG4660.1292939606402548No Hit
TCCCCACACCGTTTCTGTCCAAGTACATATAGTATGCACTCCCACGTCTA4650.12901650578909546No Hit
CAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCAT4590.1273517766821394No Hit
CACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATG4540.1259645024263427No Hit
GCAGTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAA4510.12513213787286465No Hit
CTCTTGATTCCAACCAGGTTTGCGACCGCGTTTGCAGCTTCCTGGTGGAA4490.12457722817054595No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC4440.12318995391474921No Hit
ATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTAGTGTCGGAATT4410.12235758936127118No Hit
GCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCT4410.12235758936127118No Hit
TTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGCTTGGCTTTT4350.12069286025431511No Hit
GCATACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTT4350.12069286025431511No Hit
ACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTG4330.12013795055199643No Hit
GACATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTG4310.11958304084967773No Hit
CCTTTTTTGTGATGGCAGGTTCCGTACACAACCCAAGTTGACGTCGTGTT4300.1193055859985184No Hit
GTGCATAGGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGA4260.11819576659388101No Hit
GCTAGCAACAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTT4210.1168084923380843No Hit
ATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTG4150.11514376323112821No Hit
ATGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGT4130.11458885352880951No Hit
CATCAGGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCA4080.11320157927301279No Hit
GGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCA4070.11292412442185346No Hit
AGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTTGGATTT4040.11209175986837541No Hit
GAGCTATGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCG4000.11098194046373804No Hit
CCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGG4000.11098194046373804No Hit
GATATGGCCTCCCCAGCATCGTTTCTGTCCAAGTACATATAGTATGCACT3910.10848484680330395No Hit
GTCCAAGTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCA3900.10820739195214457No Hit
GGCATACAGCATCAGGTGCATAGGAGTCAGCAATAGGGACTTTGTGGAAG3880.10765248224982589No Hit
ATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGT3870.10737502739866654No Hit
AATCAAGTGCTTTGTGTATTCTCTTGATTCCAACCAGGTTTGCGACCGCG3860.1070975725475072No Hit
GGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAA3840.10654266284518851No Hit
GCCATAGCTGTGGTCAGCAGGAGGCCAACAATTCCGACACTAGTATCTGC3840.10654266284518851No Hit
ATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAAGTGT3830.10626520799402916No Hit
CCTGACATACCTTCCACAAAGTCCCTATTGCTGACTCCTATGCACCTGAT3800.10543284344055114No Hit
GCAGTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAA3790.10515538858939179No Hit
AGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCAGC3790.10515538858939179No Hit
CATTGGGGATGAATAAGTGTTATATACAGATCATGGATCTTGGACACATG3760.10432302403591376No Hit
CCCCAGCATCGTTTCTGTCCAAGTACATATAGTATGCACTCCCACGTCTA3760.10432302403591376No Hit
CGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCAGCAGGAGGCCAACAAT3730.10349065948243573No Hit
ATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCA3720.10321320463127638No Hit
GATCTGTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGG3660.10154847552432031No Hit
GGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAG3620.10043865611968292No Hit
GAGAATAATCAATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTA3610.10016120126852357No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAG100.007088993145.000021
ACCCGGT100.007088993145.00002145
AGCTATT100.007088993145.000021
ACAGTTC458.367351E-11112.777793
CTATCCA203.8640283E-4108.750013
TCGGAGA203.8640283E-4108.750014
ATCCACT203.8640283E-4108.750015
AACCTCT203.8640283E-4108.750015
GCTCGGA203.8640283E-4108.750012
ACACCTC1350.0102.037034
ACGTTGT259.381843E-487.08
CCCCAAC259.381843E-487.01
CACACCT1600.086.093763
TCGAGTT1300.083.653853
ACCTCTG1950.081.7948766
CGAGTTT1350.080.555554
GTTCGAG1350.080.555551
TTCGAGT1350.080.555552
CAGTTCG651.5606929E-978.076934
CAATTAA406.6574095E-572.500011
Produced by FastQC (version 0.11.2)