FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_zika-her-dmso.3520000008bddb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_zika-her-dmso.3520000008bddb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453650
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC35390.7801168301554062No Hit
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA35040.7724016312134906No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC30610.6747492560343877No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC25640.5651934310591865No Hit
GTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTG25400.5599030089275874No Hit
GTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC24090.5310261214592747No Hit
GTCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT23880.5263970020941254No Hit
CCCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA18690.41199162349829166No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAA16840.3712112862338808No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC15650.34497960983136783No Hit
GTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT15130.3335170285462361No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGG14730.324699658326904No Hit
TCCCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTG14210.3132370770417723No Hit
CTCAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGA13010.28678496638377604No Hit
TACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGT12610.27796759616444394No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC12580.27730629339799406No Hit
GTGTTGGGCAGCGGCTGTCAGAAGCCATGTCTGATATTGATGCCTCATAG12040.2654028436018957No Hit
TCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTC11410.2515154855064477No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG11160.24600462911936516No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC10810.23828943017744955No Hit
GTGTATTCTCTTGATTCCAACCAGGTTTGCGACCGCGTTTGCAGCTTCCT10060.2217568610162019No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA10060.2217568610162019No Hit
GCTTCCTGGTGGAATGGGAGGGGAGCGTCACAGCTCTTCTAGATCTCCGT9940.2191116499504023No Hit
AGTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCT9930.218891215694919No Hit
GTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGA9690.21360079356331974No Hit
GTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGATTGCTTGTCAA9680.21338035930783644No Hit
CCCACACCTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA9670.21315992505235312No Hit
ATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCCCC9610.2118373195194533No Hit
CATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGG9610.2118373195194533No Hit
CAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAAT9500.209412542709137No Hit
GTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTTG9430.2078695029207539No Hit
TGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGC9170.202138212278188No Hit
GAATAAGTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCA9110.20081560674528823No Hit
CATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGG9100.20059517248980493No Hit
TCCGTACACAACCCAAGTTGACGTCGTGTTGCACCAACAATCGACGTCAT9010.19861126419045522No Hit
GTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAG8430.18582607737242368No Hit
TACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCC8190.18053565524082443No Hit
GTCAATATGCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTT8140.1794334839634079No Hit
ATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGA8110.178772181196958No Hit
GCATAGGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACT8070.17789044417502478No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC8030.1770087071530916No Hit
GGGTATGGCTATTGGGTTCATGCCACAGATGGTCATCAGGACCACCTTGA7710.1699548109776259No Hit
GTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTC7630.1681913369337595No Hit
CCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGAT7540.1662074286344098No Hit
ATGCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAG7450.16422352033506005No Hit
TCCCCAATGTGGTTGGAAAAGATATGGCCTCCCCAGCATCGTTTCTGTCC7430.16378265182409346No Hit
ATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCA7410.16334178331312688No Hit
GTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACT7370.16246004629119365No Hit
CAATCAAGTCCTAGGCTTCCAAAACCCCCCAGGGTGGCTTCGGCTCTCGG7090.1562878871376612No Hit
CTTCTAGATCTCCGTGCTTCACCTTTTTTGTGATGGCAGGTTCCGTACAC7050.15540615011572798No Hit
TCTTTATTGTTTCCATAGCCTCTTTTTTCCCCACTGAACCCCATCTATTG6960.15342224181637826No Hit
ATATCAGACATGGCTTCTGACAGCCGCTGCCCAACACAAGGTGAAGCCTA6940.15298137330541167No Hit
GAATAATCAATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTAGT6850.15099746500606193No Hit
ATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCAT6800.14989529372864543No Hit
ATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACCAGGAAGCTGCAAA6760.14901355670671224No Hit
TCCTAGCATTGATTATTCTCAGCATGGCAGCCAGATCTTTCTTGAACTTC6660.1468092141518792No Hit
GTGTACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCTAGAAG6640.14636834564091258No Hit
CACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCCCCAGCATC6570.14482530585252948No Hit
GCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGC6560.1446048715970462No Hit
CACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGATTGCT6560.1446048715970462No Hit
CTAATCAAGTGCTTTGTGTATTCTCTTGATTCCAACCAGGTTTGCGACCG6540.14416400308607957No Hit
TCACACATGTGTCCAAGATCCATGATCTGTATATAACACTTATTCATCCC6430.14173922627576324No Hit
GAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGA6430.14173922627576324No Hit
GGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAG6400.14107792350931336No Hit
GATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACCAGGAAGCTGCAA6160.13578750137771411No Hit
ATCCAGCATAGGGCATTCATAGCTCATGGTGGCATCACACATGTGTCCAA6080.1340240273338477No Hit
GTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTGT6050.13336272456739776No Hit
CAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAAAGA5890.12983577647966493No Hit
TCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTC5870.12939490796869832No Hit
TCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTAT5870.12939490796869832No Hit
GTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGG5860.12917447371321503No Hit
TATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGGCA5830.12851317094676512No Hit
ATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTG5820.12829273669128183No Hit
AGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAG5800.12785186818031521No Hit
GTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCAGCA5790.12763143392483192No Hit
TTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGCTTGGCTTTT5790.12763143392483192No Hit
CTATTGATGAGACCCAGTGATGGCTTGATTGCCGTGAATCTCAAAAAGGC5780.12741099966934863No Hit
TAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCAG5730.1263088283919321No Hit
CTCTTGATTCCAACCAGGTTTGCGACCGCGTTTGCAGCTTCCTGGTGGAA5680.1252066571145156No Hit
GACATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTG5570.12278188030419929No Hit
CTAGTATCTGCGCCTCGTCTCTTCTTCTCCTTCCTAGCATTGATTATTCT5530.12190014328226607No Hit
ATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGCAA5470.12057753774936625No Hit
GGTTTGCGACCGCGTTTGCAGCTTCCTGGTGGAATGGGAGGGGAGCGTCA5400.11903449796098313No Hit
GGCATACAGCATCAGGTGCATAGGAGTCAGCAATAGGGACTTTGTGGAAG5350.11793232668356664No Hit
TCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAAAG5270.11616885263970021No Hit
GTACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCTAGAAGAG5260.1159484183842169No Hit
ATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTAT5220.1150666813622837No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC5130.11308277306293399No Hit
CAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCAT5120.11286233880745068No Hit
CCCCAGCCTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA5120.11286233880745068No Hit
ACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTG5110.11264190455196738No Hit
ATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTAGTGTCGGAATT5110.11264190455196738No Hit
TCCCCACACTTGTCTAGGATTGGAGATCCTGAAGTTCCTGCTGGGTAATC5110.11264190455196738No Hit
GCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCT5110.11264190455196738No Hit
GCATACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTT5100.11242147029648407No Hit
CTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTTTT5030.11087843050810095No Hit
CCTTTTTTGTGATGGCAGGTTCCGTACACAACCCAAGTTGACGTCGTGTT5000.11021712774165104No Hit
GCAGTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAA4950.10911495646423455No Hit
GGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACACGACGT4890.10779235093133473No Hit
CATCAGGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCA4870.10735148242036813No Hit
CACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATG4840.10669017965391822No Hit
AGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTTGGATTT4820.10624931114295161No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC4820.10624931114295161No Hit
GGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAG4790.1055880083765017No Hit
ATGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGT4790.1055880083765017No Hit
ATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAAGTGT4770.10514713986553512No Hit
GCTAGCAACAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTT4770.10514713986553512No Hit
AATCAAGTGCTTTGTGTATTCTCTTGATTCCAACCAGGTTTGCGACCGCG4740.10448583709908521No Hit
CCCTAGAACCACGACAGTTTGCCTTTTGGCATGTGCGTCCTTGAACTCTA4730.1042654028436019No Hit
CATTGGGGATGAATAAGTGTTATATACAGATCATGGATCTTGGACACATG4710.10382453433263529No Hit
GGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAA4700.103604100077152No Hit
GATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAG4680.10316323156618538No Hit
CTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTG4670.10294279731070208No Hit
GATATGGCCTCCCCAGCATCGTTTCTGTCCAAGTACATATAGTATGCACT4670.10294279731070208No Hit
GCAGTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAA4590.10117932326683565No Hit
ATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCA4580.10095888901135236No Hit
CCTGACATACCTTCCACAAAGTCCCTATTGCTGACTCCTATGCACCTGAT4570.10073845475586907No Hit
GCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATAT4560.10051802050038577No Hit
TTCCAAGACAACATCAACCCAAGTCCCACCTGACATACCTTCCACAAAGT4540.10007715198941916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGT100.0070895934145.02
GCGACAG202.12934E-6145.01
CTATAAA100.0070895934145.01
CCTAATT100.0070895934145.01
TGCGCTC100.0070895934145.02
TACTCAG100.0070895934145.03
TATAAAT100.0070895934145.02
CAATAAG100.0070895934145.02
TAACCAA203.8646802E-4108.757
CCCCAAC301.5985132E-596.666661
AACCTCT301.5985132E-596.666665
ACCCTCA259.383418E-487.02
CGAGTTT1750.082.857154
CACACTT1150.081.956535
TCGAGTT1800.080.555563
TTCGAGT1900.076.315782
ACACTTG1250.075.46
GTTCGAG1950.074.358981
CCAACCT406.659266E-572.53
ATAACCA300.001935058672.499996
Produced by FastQC (version 0.11.2)