FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_zika-her-be.3520000008bdce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_zika-her-be.3520000008bdce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507356
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC26130.515022981890428No Hit
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA25810.5087157735396841No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC19190.37823540078367063No Hit
GTCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT17780.35044426398820555No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC17670.3482761611176373No Hit
GTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC16940.3338878420675029No Hit
CCCCAGCCTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA13030.2568216400318514No Hit
GTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTG12080.23809711524058058No Hit
GGGAAATCCATGGGTTCGGGGTATTTCACACTAGTGTCTGGCTCAAGGTT11990.23632321289193386No Hit
GAGTGTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCTGGTGACA11830.23316960871656192No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC11820.2329725084556012No Hit
CCCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA11730.2311986061069545No Hit
GTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT11270.2221319941027602No Hit
GAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCTGGTGACA11130.21937259044930976No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC11010.2170073873177808No Hit
TCCCCACACTTGTCTAGGATTGGAGATCCTGAAGTTCCTGCTGGGTAATC10770.21227698105472292No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGG9890.1949321580901773No Hit
CTCAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGA9520.1876394484346297No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC9290.1831061424325326No Hit
TGATCCTCCACAAGAAAGCTGTTCCATGCTCTATGTTTGAGTGGGCATTC9200.18133224008388588No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAA8840.17423663068929904No Hit
TCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTC8700.17147722703584858No Hit
GATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAG8430.16615551998990846No Hit
CTTCTATTCCATCTGTCCACAATGTGTGGGACTTTGGCCATTCACATGTT8240.1624106150316543No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC8160.16083381294396834No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG8140.16043961242204685No Hit
GTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGG7780.15334400302746No Hit
GTGTATTCTCTTGATTCCAACCAGGTTTGCGACCGCGTTTGCAGCTTCCT7300.1438831905013442No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA7220.14230638841365825No Hit
GGGTATGGCTATTGGGTTCATGCCACAGATGGTCATCAGGACCACCTTGA7030.1385614834554041No Hit
GAATAAGTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCA7010.1381672829334826No Hit
GTGTTGGGCAGCGGCTGTCAGAAGCCATGTCTGATATTGATGCCTCATAG6740.13284557588754248No Hit
ATGTTTGAGTGGGCATTCCTTCAGTGTGTCACCATCCACGACAAAGCTGT6730.13264847562658172No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC6710.13225427510466023No Hit
ATATCAGACATGGCTTCTGACAGCCGCTGCCCAACACAAGGTGAAGCCTA6580.12969197171217053No Hit
TGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGC6160.12141376075181923No Hit
TCCGTACACAACCCAAGTTGACGTCGTGTTGCACCAACAATCGACGTCAT6130.120822459968937No Hit
CTGCTGATGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGC6030.11885145735932953No Hit
CTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTG5850.11530365266203613No Hit
TACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCC5850.11530365266203613No Hit
GTAGAAGGGGAGCTCAACGCAATCCTGGAAGAGAATGGAGTTCAACTGAC5850.11530365266203613No Hit
GTGTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCTGGTGACATG5850.11530365266203613No Hit
GGAGTTACAGTACGCAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGA5830.11490945214011462No Hit
CTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGGTTCGG5740.11313554979146792No Hit
TACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGT5740.11313554979146792No Hit
ATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGA5660.11155874770378195No Hit
CAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAAT5640.11116454718186047No Hit
CTGTACCTCCACTGTGACTGTCCCGTGCAGTGTTTCAGCCGGGATCTTGG5610.11057324639897823No Hit
CTCTGGTATTGTGATGGCTGAGTGGCCCAGCTAAAGACTTGGGTATGATC5570.10978484535513525No Hit
CATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGG5550.10939064483321377No Hit
GTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAG5540.10919354457225301No Hit
ATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCCCC5520.10879934405033152No Hit
CTTCTAGATCTCCGTGCTTCACCTTTTTTGTGATGGCAGGTTCCGTACAC5470.1078138427455278No Hit
GAATAGAAGAGAGTGATCTGATCATACCCAAGTCTTTAGCTGGGCCACTC5410.10663124117976332No Hit
CCCTAGAACCACGACAGTTTGCCTTTTGGCATGTGCGTCCTTGAACTCTA5400.10643414091880259No Hit
TATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGGCA5290.10426603804823437No Hit
ATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGAAAG5230.10308343648246991No Hit
GCATAGGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACT5180.10209793517766619No Hit
GCTAAAGACTTGGGTATGATCAGATCACTCTCTTCTATTCCATCTGTCCA5170.10190083491670543No Hit
CTCTTACATTGTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGC5170.10190083491670543No Hit
CTCTATATCTGCCTTGGCGAACCCTCCAGCCAATGCGCATATCAGGCCAA5110.10071823335094096No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG100.0070898393145.000026
TGTTCGA100.0070898393145.000022
TCGAGTG100.0070898393145.000025
GTCATAA3650.087.397261
CTATAAG300.001935190872.53
CACTTGT2800.067.321437
CGAGTTT554.058722E-665.909094
ACACTTG2900.065.06
TCATAAT5000.063.8000032
AATACTG5150.063.3495186
ATAATAC5050.063.1683164
ACTTGTC3000.062.8333328
TAATACT5200.062.7403835
GAAGCGA1051.6370905E-1162.1428576
TGAAGCG1200.060.4166685
TCGAGTT606.8007776E-660.4166683
CATAATA5550.057.4774783
TTATGAC4800.055.8854184
ATTATGA5050.054.5544553
CAGTTCG400.00604917454.3750041
Produced by FastQC (version 0.11.2)