FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_negative_rt-pcr_control_l_cb7-9_2.3520000008bca6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_negative_rt-pcr_control_l_cb7-9_2.3520000008bca6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATTGTGTGAACCCGGGAGACAGAGGTTGTGGTGAGCCGAGATTGT130.8795669824086604No Hit
TAGTAGGACCCTCTGCAGCCAAACTAGCATATCCTGAGCCAGGCTGACCT90.6089309878213802No Hit
ACTAAGAGCCAGGACTGAGGAGCAAGGCCCAAAAAACTCCTCTGGGCCAC90.6089309878213802No Hit
ATCCTACTGTACCTGCTGCACCTCATCTGACACCAGCTTCTCTGCTTTGC70.4736129905277402No Hit
AGCATATCCTGAGCCAGGCTGACCTTAAAAAGTAAGCATTCAGGGCCTAG60.40595399188092013No Hit
GGATATGCTAGTTTGGCTGCAGAGGGTCCTACTAAGAGCCAGGACTGAGG60.40595399188092013No Hit
TCTTGGGGCTGCACTGGCGACCTGAGAACACCTTATCCTACTGTACCTGC60.40595399188092013No Hit
CCTCTAGCCCCACCCGCTGTCCCCTGCTCCTCATGCCCCCATGCTGTGTC60.40595399188092013No Hit
GCAGAGGGTCCTACTAAGAGCCAGGACTGAGGAGCAAGGCCCAAAAAACT50.3382949932341001No Hit
CCCTAGGCCCTGAATGCTTACTTTTTAAGGTCAGCCTGGCTCAGGATATG50.3382949932341001No Hit
GACAGAGGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCTGGGT50.3382949932341001No Hit
AAGCAGGATAATTGTGTGAACCCGGGAGACAGAGGTTGTGGTGAGCCGAG50.3382949932341001No Hit
ATCCTGAGCCAGGCTGACCTTAAAAAGTAAGCATTCAGGGCCTAGGGACC50.3382949932341001No Hit
GTCCTGGCTCTTAGTAGGACCCTCTGCAGCCAAACTAGCATATCCTGAGC50.3382949932341001No Hit
GTCCTACTAAGAGCCAGGACTGAGGAGCAAGGCCCAAAAAACTCCTCTGG50.3382949932341001No Hit
CTCCTAGCAAAGGGCTCAGCAGCACACGGCCTCCCTGCGGGGATGCCCTC50.3382949932341001No Hit
GAATGAAGCAAGATCGTCATGTGGGAGACACTGACTACCCACTCCTAGCA40.2706359945872801No Hit
GTAGAAACAAGGTCTCACCATATTGCCTAGGCTGGTCTTGAACTCTTGGC40.2706359945872801No Hit
GCCTCCCTGCGGGGATGCCCTCGGGGAAGATGTGGCCCAGAGGAGTTTTT40.2706359945872801No Hit
CCCCCATGCTGTGTCCCCTGGCCTCACTTCCGTTGCCATGTTTTCATAAT40.2706359945872801No Hit
TCCCTAGGCCCTGAATGCTTACTTTTTAAGGTCAGCCTGGCTCAGGATAT40.2706359945872801No Hit
ACCCACTCCTAGCAAAGGGCTCAGCAGCACACGGCCTCCCTGCGGGGATG40.2706359945872801No Hit
CAGCAGCACACGGCCTCCCTGCGGGGATGCCCTCGGGGAAGATGTGGCCC40.2706359945872801No Hit
AGCCAGGACTGAGGAGCAAGGCCCAAAAAACTCCTCTGGGCCACATCTTC40.2706359945872801No Hit
AGCGAAAGCAGGAGGATCACTTGAGGCCAAGAGTTCAAGACCAGCCTAGG40.2706359945872801No Hit
AGACAGAGGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCTGGG30.20297699594046006No Hit
AAGCAGAGGCCTCCCTAAACCTCCCTCTCCTCTAGCCCCACCCGCTGTCC30.20297699594046006No Hit
GACTACCCACTCCTAGCAAAGGGCTCAGCAGCACACGGCCTCCCTGCGGG30.20297699594046006No Hit
GCTCAGCAGCACACGGCCTCCCTGCGGGGATGCCCTCGGGGAAGATGTGG30.20297699594046006No Hit
CATCTTCCCCGAGGGCATCCCCGCAGGGAGGCCGTGTGCTGCTGAGCCCT30.20297699594046006No Hit
CTCTGTCTCCAAATAAAAAAAAAAAAGGAAAAGAAATTCCATCCCAAGGT30.20297699594046006No Hit
AGCGAAAGCAGAGGCCTCCCTAAACCTCCCTCTCCTCTAGCCCCACCCGC30.20297699594046006No Hit
GCCCCAAGATGAGCCTGCAGTATTTTCCTTACATGATCTGGTCCTACTGT30.20297699594046006No Hit
AGCAGAGGCCTCCCTAAACCTCCCTCTCCTCTAGCCCCACCCGCTGTCCC30.20297699594046006No Hit
GTGTGAACCCGGGAGACAGAGGTTGTGGTGAGCCGAGATTGTGCCATTGC30.20297699594046006No Hit
CAGTAGGACCAGATCATGTAAGGAAAATACTGCAGGCTCATCTTGGGGCT30.20297699594046006No Hit
CACCTTATCCTACTGTACCTGCTGCACCTCATCTGACACCAGCTTCTCTG30.20297699594046006No Hit
CTACTGTACCTGCTGCACCTCATCTGACACCAGCTTCTCTGCTTTGCCCA30.20297699594046006No Hit
CAGGAAGTGACTCAGAAAAGCAGTGAGCTACGAAAGCTATCTGAATGAAG30.20297699594046006No Hit
TCCCCGCAGGGAGGCCGTGTGCTGCTGAGCCCTTTGCTAGGAGTGGGTAG30.20297699594046006No Hit
GCTGAGCCCTTTGCTAGGAGTGGGTAGTCAGTGTCTCCCACATGACGATC30.20297699594046006No Hit
CTAAGAGCCAGGACTGAGGAGCAAGGCCCAAAAAACTCCTCTGGGCCACA30.20297699594046006No Hit
ATCCCTCACAGGAAGTGACTCAGAAAAGCAGTGAGCTACGAAAGCTATCT30.20297699594046006No Hit
TCCCAAGGTGATCCCTCACAGGAAGTGACTCAGAAAAGCAGTGAGCTACG30.20297699594046006No Hit
CATCTGACACCAGCTTCTCTGCTTTGCCCAAGGTCCCTAGGCCCTGAATG30.20297699594046006No Hit
TCCTGAGCCAGGCTGACCTTAAAAAGTAAGCATTCAGGGCCTAGGGACCT30.20297699594046006No Hit
ATCCCAAGGTGATCCCTCACAGGAAGTGACTCAGAAAAGCAGTGAGCTAC30.20297699594046006No Hit
GCCCAAGGTCCCTAGGCCCTGAATGCTTACTTTTTAAGGTCAGCCTGGCT30.20297699594046006No Hit
AAAAACTCCTCTGGGCCACATCTTCCCCGAGGGCATCCCCGCAGGGAGGC30.20297699594046006No Hit
GCACTGGCGACCTGAGAACACCTTATCCTACTGTACCTGCTGCACCTCAT30.20297699594046006No Hit
TTATCCTACTGTACCTGCTGCACCTCATCTGACACCAGCTTCTCTGCTTT30.20297699594046006No Hit
TTCATAATCCTCTCAGGCTCTGGGCAGCAGCGCTGCCCACAGTAGGACCA30.20297699594046006No Hit
GTCCTACTGTGGGCAGCGCTGCTGCCCAGAGCCTGAGAGGATTATGAAAA30.20297699594046006No Hit
GCACACGGCCTCCCTGCGGGGATGCCCTCGGGGAAGATGTGGCCCAGAGG30.20297699594046006No Hit
AGCGAAAGCAGGATAATTGTGTGAACCCGGGAGACAGAGGTTGTGGTGAG30.20297699594046006No Hit
GGGTAGTCAGTGTCTCCCACATGACGATCTTGCTTCATTCAGATAGCTTT30.20297699594046006No Hit
GTGCGAGACTCTGTCTCCAAATAAAAAAAAAAAAGGAAAAGAAATTCCAT30.20297699594046006No Hit
CCGGGAGACAGAGGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCAGC30.20297699594046006No Hit
ATATTGCCTAGGCTGGTCTTGAACTCTTGGCCTCAAGTGATCCTCCTGCT20.13531799729364005No Hit
GTGTCTCCCACATGACGATCTTGCTTCATTCAGATAGCTTTCGTAGCTCA20.13531799729364005No Hit
GGTGATCCCTCACAGGAAGTGACTCAGAAAAGCAGTGAGCTACGAAAGCT20.13531799729364005No Hit
ACTGTACCTGCTGCACCTCATCTGACACCAGCTTCTCTGCTTTGCCCAAG20.13531799729364005No Hit
ATCTTGCTTCATTCAGATAGCTTTCGTAGCTCACTGCTTTTCTGAGTCAC20.13531799729364005No Hit
GGCCTCCCTAAACCTCCCTCTCCTCTAGCCCCACCCGCTGTCCCCTGCTC20.13531799729364005No Hit
GTAGGACCCTCTGCAGCCAAACTAGCATATCCTGAGCCAGGCTGACCTTA20.13531799729364005No Hit
ACCCGGGAGACAGAGGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCA20.13531799729364005No Hit
GCCCACAGCAGGCGGCGGCGCGGCGTCGCGTGGAGCGTCGGTTCCTTGAC20.13531799729364005No Hit
GGCGATCGCGTCCTCGAGCTTGTCGAAGCCGCGGATCGCCGCGACCGGGC20.13531799729364005No Hit
GAAGTGGTGGTAGTAGGTCAGTACGCCGTGGACTTCAGCACGCGACAGGT20.13531799729364005No Hit
TTTAAGGTCAGCCTGGCTCAGGATATGCTAGTTTGGCTGCAGAGGGTCCT20.13531799729364005No Hit
CCATATTGCCTAGGCTGGTCTTGAACTCTTGGCCTCAAGTGATCCTCCTG20.13531799729364005No Hit
ACGAAAGCTATCTGAATGAAGCAAGATCGTCATGTGGGAGACACTGACTA20.13531799729364005No Hit
GATAGCTTTCGTAGCTCACTGCTTTTCTGAGTCACTTCCTGTGAGGGATC20.13531799729364005No Hit
CATTCAGGGCCTAGGGACCTTGGGCAAAGCAGAGAAGCTGGTGTCAGATG20.13531799729364005No Hit
GGTCCTACTAAGAGCCAGGACTGAGGAGCAAGGCCCAAAAAACTCCTCTG20.13531799729364005No Hit
GGGAGACAGAGGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCT20.13531799729364005No Hit
GGGCTCAGCAGCACACGGCCTCCCTGCGGGGATGCCCTCGGGGAAGATGT20.13531799729364005No Hit
CTACCCACTCCTAGCAAAGGGCTCAGCAGCACACGGCCTCCCTGCGGGGA20.13531799729364005No Hit
CTCTCAGGCTCTGGGCAGCAGCGCTGCCCACAGTAGGACCAGATCATGTA20.13531799729364005No Hit
CTTCTCTGCTTTGCCCAAGGTCCCTAGGCCCTGAATGCTTACTTTTTAAG20.13531799729364005No Hit
ATTCCATCCCAAGGTGATCCCTCACAGGAAGTGACTCAGAAAAGCAGTGA20.13531799729364005No Hit
AGGCTGACCTTAAAAAGTAAGCATTCAGGGCCTAGGGACCTTGGGCAAAG20.13531799729364005No Hit
CCTCACAGGAAGTGACTCAGAAAAGCAGTGAGCTACGAAAGCTATCTGAA20.13531799729364005No Hit
AACAAGGTCTCACCATATTGCCTAGGCTGGTCTTGAACTCTTGGCCTCAA20.13531799729364005No Hit
GGCTCAGGATATGCTAGTTTGGCTGCAGAGGGTCCTACTAAGAGCCAGGA20.13531799729364005No Hit
GCCCAGAGGAGTTTTTTGGGCCTTGCTCCTCAGTCCTGGCTCTTAGTAGG20.13531799729364005No Hit
GGGCCACATCTTCCCCGAGGGCATCCCCGCAGGGAGGCCGTGTGCTGCTG20.13531799729364005No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGCTTGAAGGAGGAAAACCA20.13531799729364005No Hit
GCCCTGAATGCTTACTTTTTAAGGTCAGCCTGGCTCAGGATATGCTAGTT20.13531799729364005No Hit
CGAAAGCAGAGGCCTCCCTAAACCTCCCTCTCCTCTAGCCCCACCCGCTG20.13531799729364005No Hit
ATTGTGTGAACCCGGGAGACAGAGGTTGTGGTGAGCCGAGATTGTGCCAT20.13531799729364005No Hit
GTGGGTAGTCAGTGTCTCCCACATGACGATCTTGCTTCATTCAGATAGCT20.13531799729364005No Hit
GCCTGGCTCAGGATATGCTAGTTTGGCTGCAGAGGGTCCTACTAAGAGCC20.13531799729364005No Hit
GGACTGAGGAGCAAGGCCCAAAAAACTCCTCTGGGCCACATCTTCCCCGA20.13531799729364005No Hit
AGTAGAAACAAGGCAGAAGGGGTCCTGCTTTCGCTCTGTCTCTTATACAC20.13531799729364005No Hit
ATGTGGGAGACACTGACTACCCACTCCTAGCAAAGGGCTCAGCAGCACAC20.13531799729364005No Hit
AGCCGAGATTGTGCCATTGCACTCCAGCCTGGGTGACAGTGCGAGACTCT20.13531799729364005No Hit
GGCGTGCTGCCGTCTTCACCGGCGACGAACACGTTCACGCCCTTCGCCTG20.13531799729364005No Hit
TATCTGAATGAAGCAAGATCGTCATGTGGGAGACACTGACTACCCACTCC20.13531799729364005No Hit
GATTGTGCCATTGCACTCCAGCCTGGGTGACAGTGCGAGACTCTGTCTCC20.13531799729364005No Hit
GGGAGACACTGACTACCCACTCCTAGCAAAGGGCTCAGCAGCACACGGCC20.13531799729364005No Hit
CCTCTCAGGCTCTGGGCAGCAGCGCTGCCCACAGTAGGACCAGATCATGT20.13531799729364005No Hit
TCTGACACCAGCTTCTCTGCTTTGCCCAAGGTCCCTAGGCCCTGAATGCT20.13531799729364005No Hit
GCTGCACCTCATCTGACACCAGCTTCTCTGCTTTGCCCAAGGTCCCTAGG20.13531799729364005No Hit
AGTAGAAACAAGGCTTGAAGGAGGAAAACCATTTGATCCCTGCTTTTGCT20.13531799729364005No Hit
GGGTCCTACTAAGAGCCAGGACTGAGGAGCAAGGCCCAAAAAACTCCTCT20.13531799729364005No Hit
AAGAAATTCCATCCCAAGGTGATCCCTCACAGGAAGTGACTCAGAAAAGC20.13531799729364005No Hit
CTACTAAGAGCCAGGACTGAGGAGCAAGGCCCAAAAAACTCCTCTGGGCC20.13531799729364005No Hit
GTCTCCAAATAAAAAAAAAAAGGAAAAGAAATTCCATCCCAAGGTGATCC20.13531799729364005No Hit
GGGCAAAGCAGAGAAGCTGGTGTCAGATGAGGTGCAGCAGGTACAGTAGG20.13531799729364005No Hit
CACAGGAAGTGACTCAGAAAAGCAGTGAGCTACGAAAGCTATCTGAATGA20.13531799729364005No Hit
GCACTCCAGCCTGGGTGACAGTGCGAGACTCTGTCTCCAAATAAAAAAAA20.13531799729364005No Hit
GTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCTGGGTGACAGTGCGA20.13531799729364005No Hit
GGCCACATCTTCCCCGAGGGCATCCCCGCAGGGAGGCCGTGTGCTGCTGA20.13531799729364005No Hit
TGGCTGCAGAGGGTCCTACTAAGAGCCAGGACTGAGGAGCAAGGCCCAAA20.13531799729364005No Hit
GCTCTGGGCAGCAGCGCTGCCCACAGTAGGACCAGATCATGTAAGGAAAA20.13531799729364005No Hit
GAAAAGCAGTGAGCTACGAAAGCTATCTGAATGAAGCAAGATCGTCATGT20.13531799729364005No Hit
TTTTTAAGGTCAGCCTGGCTCAGGATATGCTAGTTTGGCTGCAGAGGGTC20.13531799729364005No Hit
GAGGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCTGGGTGACA20.13531799729364005No Hit
TGCTAGGAGTGGGTAGTCAGTGTCTCCCACATGACGATCTTGCTTCATTC20.13531799729364005No Hit
GGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCTGGGTGACAGT20.13531799729364005No Hit
CCCTAAACCTCCCTCTCCTCTAGCCCCACCCGCTGTCCCCTGCTCCTCAT20.13531799729364005No Hit
CAGGATAATTGTGTGAACCCGGGAGACAGAGGTTGTGGTGAGCCGAGATT20.13531799729364005No Hit
GGATAAGGTGTTCTCAGGTCGCCAGTGCAGCCCCAAGATGAGCCTGCAGT20.13531799729364005No Hit
TCTCTGCTTTGCCCAAGGTCCCTAGGCCCTGAATGCTTACTTTTTAAGGT20.13531799729364005No Hit
GCTTTTCTGAGTCACTTCCTGTGAGGGATCACCTTGGGATGGAATTTCTT20.13531799729364005No Hit
TAATTGTGTGAACCCGGGAGACAGAGGTTGTGGTGAGCCGAGATTGTGCC20.13531799729364005No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGT100.0063819443145.04
Produced by FastQC (version 0.11.2)