FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_hrv14-5c-c3-4_52c.3520000008bd3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_hrv14-5c-c3-4_52c.3520000008bd3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386802
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT12940.3345380840843636No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG11620.3004120971453095No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT10130.26189109673683175No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC9480.24508663347138845No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT8510.22000920367526539No Hit
GGCTAGGACACGGTCACCCGTGGGGAATTCCTAGCCTCATCGACCAAACT7820.20217061959348712No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA7730.19984384775673342No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT7150.1848490959198763No Hit
CTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTTAAAATT6420.16597639102176306No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA6390.16520080040951185No Hit
CTGGTACTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTT6210.16054725673600448No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG6150.158996075511502No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT6130.15847901510333454No Hit
ATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTTAAAATTCC6070.15692783387883208No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG5920.15304988081757592No Hit
TATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGG5270.13624541755213262No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT5190.13417717591946268No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC5050.13055775306229025No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC5050.13055775306229025No Hit
GGTATAGGCTGTACCCACTGCCAAAAGCCTTTAACCGTTATCCGCCAACC4910.12693833020511786No Hit
TTCATATTGTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGA4710.121767726123443No Hit
ACCTAAAAGAGGTCCAACCAGCGCCTCCTAAGTACGCTGTATTCTGGGAG4680.12099213551119178No Hit
ATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAA4600.11892389387852181No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA4570.1181483032662706No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG4360.112719168980512No Hit
CTCTGGTACTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCC4310.11142651796009329No Hit
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA4260.11013386693967456No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG4110.10625591387841843No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG4020.10392914204166474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAA100.0070891934145.03
CTATCGC100.0070891934145.01
GCATATG100.0070891934145.03
CTCCTAC700.0124.285711
TCCTACT850.0102.3529362
CTACTTC950.091.578954
CTCTGGT651.6370905E-1189.230771
TACTTCT1050.082.857145
CTTCTAC1150.075.6521767
CTGGTAC1250.075.41
TTCTACT1450.060.08
ACTATGT2000.050.756
CCGTCGC1004.6931746E-850.75145
ACAATCA1953.6379788E-1240.89743145
GATATAG750.001482260938.6666641
TTATAGG4100.033.597562
CCTTTAT1751.006465E-733.1428571
GGGAGTG4200.032.797627
ATGTACC3100.032.7419361
TATAGGG4250.032.4117623
Produced by FastQC (version 0.11.2)