FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_hrv14-5c-c3-1_57c.3520000008bd55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_hrv14-5c-c3-1_57c.3520000008bd55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491729
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT10930.22227690455515128No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG9230.18770501638097406No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT9040.18384109946738955No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT8730.1775368139768043No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC8600.17489308135172016No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG8400.17082580039005224No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT8290.1685887958611349No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG8250.16777533966880132No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA7050.14337165389879386No Hit
GGCTTGAAGTATCTCTTTAAAAATGTCAAGTTTTCCCATGTCATTTTTGT6880.13991446508137614No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT6820.13869428079287574No Hit
GGACAATTCTTGACCACAGATGACAGGCAATCCCCCAGTGCACTGCCAAA6370.12954289862912297No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA6310.1283227143406226No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA5970.12140833670578711No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC5540.11266368263820113No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC5510.11205359049395094No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG5460.11103677025353395No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG5410.11001995001311698No Hit
GTATTCTGGGAGAATTAGACATTTTCCAATGTCAGTAGTTCTGATCTTCT5260.10696948929186603No Hit
TTCATATTGTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGA5080.10330893642636492No Hit
ACCCAAACCTGTGATGTAATCACTCAGCCCCTGTTCCTCTGCGATACACT5060.10290220833019813No Hit
TATTAATACTATTCTGATGTTTGTCAGTACATTAGGTATTGTTTATGTCA5000.10168202404169777No Hit
GGCTAGGACACGGTCACCCGTGGGGAATTCCTAGCCTCATCGACCAAACT4980.10127529594553097No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG4970.10107193189744758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTCA151.2296278E-4145.000021
AACCGGC100.007089774145.02
ACTGCGC100.007089774145.07
AGCCCTC100.007089774145.01
GCTATAC100.007089774145.01
TACGGTC100.007089774145.05
TTCCGGT100.007089774145.02
ACGATGT100.007089774145.05
TCTACGA100.007089774145.02
TACGATG100.007089774145.04
CGGATAG100.007089774145.06
CTACGAT100.007089774145.03
AGTCTAT100.007089774145.01
TTAACGA100.007089774145.01
TTGCAAT100.007089774145.03
GTAAAGC100.007089774145.01
AGTCGGA100.007089774145.02
GCGGATG351.900844E-9124.285711
CGGATGG404.809408E-9108.752
TTTTAGA259.3838887E-487.02
Produced by FastQC (version 0.11.2)