FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_hrv14-5c-c3-1_52c.3520000008bd2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_hrv14-5c-c3-1_52c.3520000008bd2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428776
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT11600.27053753008563913No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG9920.23135623262496036No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT8710.20313636957292386No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT8440.19683937533817192No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC8280.19310782319905967No Hit
GGCTTGAAGTATCTCTTTAAAAATGTCAAGTTTTCCCATGTCATTTTTGT7870.18354572084258447No Hit
GTATTCTGGGAGAATTAGACATTTTCCAATGTCAGTAGTTCTGATCTTCT7720.18004739071216672No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT7240.16885273429482994No Hit
GGACAATTCTTGACCACAGATGACAGGCAATCCCCCAGTGCACTGCCAAA7110.1658208481818012No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG6830.15929063193835477No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG6380.1487956415471015No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT6200.14459764539060022No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA6180.1441312013732112No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA5480.12780566076459504No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG5220.1217418885385376No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA5100.11894322443420341No Hit
GTACCCATTGATTCAATGACACGTCACAACAATGTCTCACTGATGGTCAT4970.11591133832117469No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG4930.11497845028639662No Hit
GAGTATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTC4850.1131126742168405No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC4750.11078045412989533No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG4680.10914790006903372No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC4680.10914790006903372No Hit
ATATAAAGGAATAGATTTAGACAAACTTAAAATCTTAGCTTACGGTGATG4590.10704890199078305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCT100.0070894593145.03
TCTTCGA100.0070894593145.01
CATGACA100.0070894593145.04
ACTAGTC100.0070894593145.02
TCGCTTC100.0070894593145.04
TAAGGTA100.0070894593145.02
CCGAAAC100.0070894593145.03
TTCGAGT100.0070894593145.03
AATCGTG100.0070894593145.01
TAATCTT100.0070894593145.02
CGGATGG650.0133.846152
GCGGATG650.0133.846151
CCCTAAG203.864534E-4108.754
ACTTAGT259.3830656E-487.02
ACCTAAA350.003565114562.1428571
AGAGGTC606.7989877E-660.4166687
GGTATCC1500.058.0000048
AAGAGGT651.0925853E-555.769236
GAGGTCC1109.941505E-846.136361
GATATAG1002.7304723E-643.51
Produced by FastQC (version 0.11.2)