FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_hrv14-5c-c2-4_52c.3520000008bd11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_hrv14-5c-c2-4_52c.3520000008bd11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474016
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT22170.4677057314521029No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG20990.44281205697697973No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC16780.3539964895699723No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT15820.3337440086410585No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA11810.24914770809424153No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT9860.20800985620738538No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT9810.20695503949233782No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA9750.2056892594342807No Hit
GGCTAGGACACGGTCACCCGTGGGGAATTCCTAGCCTCATCGACCAAACT9530.20104806588807128No Hit
CTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTTAAAATT9450.19936035914399514No Hit
CTGGTACTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTT8770.18501485181934785No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT8770.18501485181934785No Hit
ATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTTAAAATTCC8670.1829052183892527No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT7920.16708296766353878No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG7660.1615979207452913No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG7450.1571676905420914No Hit
TATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGG7390.1559019104840343No Hit
ATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAA7360.15526902045500574No Hit
GGTATAGGCTGTACCCACTGCCAAAAGCCTTTAACCGTTATCCGCCAACC7300.15400324039694863No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG6900.14556470667656787No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC6800.1434550732464727No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC6340.13375075946803483No Hit
CAGTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATG6260.13206305272395868No Hit
CATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGATCT5910.12467933571862554No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT5690.12003814217241612No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT5580.11771754539931141No Hit
ATCCAATGGTGTCTATGTACAAGCACTTCTGTTTCCCCGGAGCGAGGTAT5580.11771754539931141No Hit
ACCCAAACCTGTGATGTAATCACTCAGCCCCTGTTCCTCTGCGATACACT5570.11750658205630189No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG5550.11708465537028286No Hit
ACCTAAAAGAGGTCCAACCAGCGCCTCCTAAGTACGCTGTATTCTGGGAG5550.11708465537028286No Hit
CTCTGGTACTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCC5480.11560791196921624No Hit
GTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC5310.11202153513805442No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC5180.10927901167893067No Hit
TTTAAGGACATGGTGTGAAGACTCGCATGTGCTTGGTTGTGAGTCCTCCG5080.10716937824883548No Hit
TTCATATTGTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGA5070.10695841490582596No Hit
TACATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA5050.10653648821980692No Hit
GATGTATACTGGTCCTGCCTTGTCCAGTGCTAAACTCATTCTAGCATACA5040.1063255248767974No Hit
GGGTTAAGGTTAGCCGCATTCAGGGGCCGGAGGACTCACAACCAAGCACA5010.10569263484776885No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA4970.10484878147573078No Hit
GTGTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCA4940.10421589144670221No Hit
GTATACATCAAAGAGAATCTAAGTGATCCAGACCAATGTGTATAGTACTG4900.10337203807466414No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA4860.10252818470262606No Hit
GTATAAGAGAAGTTTGATACCAACATGATAGAAAGCCAGCACTGGTGTAT4780.10084047795854993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTG100.0070896936145.05
GTCTAGG100.0070896936145.01
AAGCCGG100.0070896936145.01
AAACAGC100.0070896936145.0145
GTATACG100.0070896936145.01
TATACGT100.0070896936145.02
CTCTGGT900.0120.8333361
TCCTACT501.8189894E-12116.02
CTCCTAC501.8189894E-12116.01
CTGGTAC1750.082.857151
CTACTTC851.6916601E-1068.235294
TACTTCT902.8194336E-1064.444445
ACATAGT702.3318898E-762.1428576
GGTACAT651.0927624E-555.769233
ACAATCA2300.053.58696145
GATATAG953.1346644E-853.4210551
AGGTACA701.6946202E-551.7857132
TTCTACT1301.3096724E-1050.1923072
GACGTTG1056.8961526E-848.3333327
CTTCTAC1402.7102942E-1046.6071431
Produced by FastQC (version 0.11.2)