FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_hrv14-5c-c2-1_57c.3520000008bd48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_hrv14-5c-c2-1_57c.3520000008bd48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526791
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT13040.24753649929478674No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG12070.2291231247306807No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT10590.20102849137513737No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT9480.17995751635847992No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC9380.17805923032094323No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT9270.17597111567965285No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG8530.1619237990018812No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG8310.15774756971930043No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA8300.15755774111554677No Hit
GGACAATTCTTGACCACAGATGACAGGCAATCCCCCAGTGCACTGCCAAA8110.153950997644227No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT7780.1476866537203559No Hit
GGCTTGAAGTATCTCTTTAAAAATGTCAAGTTTTCCCATGTCATTTTTGT7620.14464939606029717No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA6590.12509704987366907No Hit
GTATTCTGGGAGAATTAGACATTTTCCAATGTCAGTAGTTCTGATCTTCT6350.12054116338358095No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA6300.11959202036481262No Hit
GAGTATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTC6200.11769373432727591No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC6070.11522596247847817No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG6010.11408699085595617No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG5940.11275819062968047No Hit
GGCTAGGACACGGTCACCCGTGGGGAATTCCTAGCCTCATCGACCAAACT5870.11142939040340476No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC5720.10858196134709971No Hit
ACCCAAACCTGTGATGTAATCACTCAGCCCCTGTTCCTCTGCGATACACT5680.10782264693208503No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG5600.10630401810205566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGT151.2296773E-4145.000022
TACCTTA151.2296773E-4145.000022
TCGGTCA151.2296773E-4145.000024
CCGTCAT100.0070899166145.04
TCGGAGA100.0070899166145.04
CTTATTG100.0070899166145.05
GCTACAG100.0070899166145.03
GCTCGGA100.0070899166145.02
CGTCATC100.0070899166145.05
CTAGGTT100.0070899166145.05
CGGAGAT100.0070899166145.05
CTCAGTT100.0070899166145.05
CGGATGG351.900844E-9124.285712
GCGGATG351.900844E-9124.285711
ACCTAAA451.09066605E-896.6666641
GATACTA259.384263E-487.01
TTCGGTC259.384263E-487.03
CGTGTAG259.384263E-487.05
TAGACGT1450.075.05
GACGTTG1450.075.07
Produced by FastQC (version 0.11.2)