FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_hrv14-5c-c2-1_52c.3520000008bd04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_hrv14-5c-c2-1_52c.3520000008bd04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506728
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT13410.2646390173821064No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG12950.2555611689111318No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC10390.20504096872483857No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT9290.1833330702072907No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT9240.18234634754740214No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT8720.17208443188456132No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG8250.16280923888160906No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG7990.15767828105018866No Hit
GGACAATTCTTGACCACAGATGACAGGCAATCCCCCAGTGCACTGCCAAA7730.15254732321876827No Hit
GTATTCTGGGAGAATTAGACATTTTCCAATGTCAGTAGTTCTGATCTTCT7720.15234997868679054No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT7510.1482057435152587No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA7460.14721902085537014No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA6900.1361677270646185No Hit
GGCTTGAAGTATCTCTTTAAAAATGTCAAGTTTTCCCATGTCATTTTTGT6750.13320755908495285No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC6270.12373502155002289No Hit
GAGTATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTC6060.11959078637849103No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA5830.11505186214300372No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG5710.11268372775927124No Hit
GTACCCATTGATTCAATGACACGTCACAACAATGTCTCACTGATGGTCAT5410.10676339179994002No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC5410.10676339179994002No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG5360.10577666914005146No Hit
CATATATTTCATCCCTAAAGATATGATGGGTATTACAAATTGATTGAATT5270.1040005683522521No Hit
GGCTAGGACACGGTCACCCGTGGGGAATTCCTAGCCTCATCGACCAAACT5180.10222446756445272No Hit
TATTAATACTATTCTGATGTTTGTCAGTACATTAGGTATTGTTTATGTCA5130.10123774490456419No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCT100.007089838145.02
GATCCTC151.2296502E-4145.01
GAGAGAT100.007089838145.04
GTATCGT100.007089838145.01
CGATGTA100.007089838145.05
CGCACTT100.007089838145.06
TGAGAGA100.007089838145.03
ATCGCCA100.007089838145.02
CCCGATG100.007089838145.03
CGGATGG403.274181E-11126.8752
GCGGATG458.367351E-11112.777781
AGAGGTC353.4356366E-582.857147
AGTATGT350.003565488262.1428572
ACCTAAA350.003565488262.1428571
TATACGT350.003565488262.1428571
GAGGTCC606.8007666E-660.4166681
GGTATCC851.3036697E-859.705888
AAGAGGT502.0092938E-458.06
TCCTCAA450.00963641348.3333321
CCTAAGT1700.046.9117621
Produced by FastQC (version 0.11.2)