FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_2427_day_5_ul-b.3520000008bc49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_2427_day_5_ul-b.3520000008bc49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286866
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA15470.5392761777275801No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC14000.48803274002495944No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA7150.24924529222703282No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA6750.2353014996548911No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG6000.20915688858212547No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA4250.14815279607900553No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC3980.13874073609280987No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC3860.13455759832116737No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA3810.13281462424964968No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA3660.12758570203509653No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT3620.12619132277788236No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA3580.1247969435206682No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA3390.11817364204890086No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC3090.1077157976197946No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA3060.10667001317688399No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC2990.10422984947675917No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2940.10248687540524147No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA2940.10248687540524147No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG2930.10213828059093794No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG2890.10074390133372375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGG100.0070882454145.06
GAGCGAA2750.0116.01
AGCAAAA15350.078.876222
CAAAAGC16150.075.417954
GCAAAAG16150.075.417953
AGCGAAA4200.074.226192
GAGCAAA17200.071.6569751
GCGAAAG4650.067.0430153
AAAGCAG24200.062.9132276
AAAAGCA19800.061.148995
CGAAAGC5200.059.951924
AAGCAGG30100.050.8222587
GGACAAT604.939146E-448.3333326
GGACCGT657.3278043E-444.6153836
GCAGGGT2650.043.7735869
AGGACCG500.001393794743.55
GAAAGCA7450.042.818795
GGATACC700.001055380241.428579
GCAGGTT1953.6379788E-1240.8974389
GCAGGAG7100.040.845079
Produced by FastQC (version 0.11.2)