FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_2420_day_3_ll-e.3520000008bc12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_2420_day_3_ll-e.3520000008bc12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326001
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT46381.4226950224079067No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG38971.1953951061499812No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA38821.190793893270266No Hit
CATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT28450.872696709519296No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC25330.776991481621222No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG24020.7368075558050435No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA17280.5300597237431787No Hit
TTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATG16440.5042929316167742No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG16250.4984647286358017No Hit
CAAGCCAAATTCATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAG15130.4641090058005957No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA13380.4104281888705863No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA12390.38006018386446666No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG11470.35183941153554743No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA10630.32607261940914295No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT10050.30828126294091124No Hit
CATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT9990.3064407777890252No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC9990.3064407777890252No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT9830.30153281738399573No Hit
GAATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCGTT9650.29601136192833766No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG9470.2904899064726795No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT9410.2886494213207935No Hit
GGATACACCATGGACACAGTAAACAGAATGATGCCGTTGCAACTACACAT9220.28282121833982105No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG9010.2763795203082199No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC8860.27177830742850484No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA8730.2677905895994184No Hit
GTTCATTGATGCTTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGT8530.2616556390931316No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA8490.2604286489918743No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC7710.2365023420173558No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA7640.2343551093401554No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT7630.23404836181484104No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT7490.22975389646044028No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA7380.22637967368198258No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT7350.22545943110603958No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT7310.2242324410047822No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG7230.22177846080226749No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC6840.20981530731500825No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC6710.2058275894859218No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG6620.20306686175809277No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT6560.20122637660620674No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC6560.20122637660620674No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC6450.19785215382774898No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT6350.1947846785746056No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG6300.19325094094803388No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG6290.19294419342271957No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC6160.18895647559363316No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG6050.1855822528151754No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC6010.18435526271391806No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT5930.18190128251140333No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA5720.17545958447980223No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT5600.17177861417603013No Hit
CAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAG5530.16963138149882975No Hit
GAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAA5520.1693246339735154No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG5250.16104245079002824No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT5250.16104245079002824No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC5200.15950871316345655No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT5080.15582774285968448No Hit
TCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGA5040.15460075275842713No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA4990.15306701513185542No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGC4960.1521467725559124No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC4800.14723881215088297No Hit
CCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTAGGGCCCGGAT4780.14662531710025428No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG4750.14570507452431128No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA4730.1450915794736826No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT4720.14478483194836828No Hit
GACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTAGGGCCCGG4710.1444780844230539No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA4690.14386458937242522No Hit
CCATCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTG4640.14233085174585353No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG4620.14171735669522487No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG4610.14141060916991052No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA4590.14079711411928184No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC4500.1380363863914528No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG4270.13098119330922298No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT4220.12944745568265129No Hit
GGATATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCGGTTGGA4130.12668672795482222No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTT4110.12607323290419356No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT4100.1257664853788792No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT4100.1257664853788792No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT4100.1257664853788792No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA4050.1242327477523075No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4040.12392600022699317No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG4030.12361925270167884No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG3960.12147202002447846No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG3960.12147202002447846No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT3950.1211652724991641No Hit
TTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCT3940.12085852497384977No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAG3880.11901803982196373No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG3850.11809779724602071No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA3830.11748430219539203No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG3820.1171775546700777No Hit
GTTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCC3780.11595056456882033No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTT3750.11503032199287733No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC3720.11411007941693431No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT3630.11134935168910524No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT3620.11104260416379091No Hit
GGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA3620.11104260416379091No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGT3610.11073585663847656No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT3600.11042910911316223No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT3580.10981561406253354No Hit
GCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT3570.10950886653721921No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3560.10920211901190487No Hit
CCGATGGTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGA3560.10920211901190487No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC3560.10920211901190487No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA3550.10889537148659054No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG3490.1070548863347045No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC3480.10674813880939016No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG3450.10582789623344714No Hit
ATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTC3440.10552114870813278No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC3420.10490765365750412No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC3410.10460090613218978No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA3400.10429415860687544No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3380.10368066355624676No Hit
GGCCTCATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAA3370.10337391603093243No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT3350.10276042098030375No Hit
TCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCT3330.10214692592967506No Hit
TACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTG3330.10214692592967506No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA3330.10214692592967506No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA3310.10153343087904639No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC3300.10122668335373206No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC3300.10122668335373206No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAGA100.0070886863144.999981
GAGCGAA3400.0138.602941
GAGCAAA16750.0134.17911
GGTTATT203.8636947E-4108.749991
CTAATAA404.80577E-9108.749999
AGCAGGC7550.099.8675468
AGCAAAA23800.095.042022
GCAGGCA7950.094.8427669
GCAGGTA3450.094.565229
AAGCAGG29650.092.428327
CCAAATT2300.091.413055
AGCAGGT11900.090.168078
GCAGGGT8100.089.506179
AGTAGAA3700.088.175671
CTCATGA259.381033E-487.03
GTGACCT259.381033E-487.03
TTATTAG259.381033E-487.03
GCAGGTC8550.086.491239
CAAATTC2500.084.16
GCCAAAT2500.084.14
Produced by FastQC (version 0.11.2)