FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_2417_day_1_ul-b.3520000008bc2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_2417_day_1_ul-b.3520000008bc2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205283
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10090.4915165892938041No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG8910.43403496636350797No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA6810.3317371628434892No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC5150.250873184822903No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC5090.24795039043661676No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA3550.17293200118860305No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG3530.1719577363931743No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA3220.1568566320640287No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2950.14370405732574057No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2830.13785846855316808No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2560.12470589381487994No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC2380.1159375106560212No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2340.1139889810651637No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2310.11252758387202057No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2310.11252758387202057No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG2290.11155331907659181No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2180.1061948627017337No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA2160.10522059790630495No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT2140.10424633311087621No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2110.10278493591773308No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2090.10181067112230432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1500.0125.666671
GCCGTTA755.4569682E-1177.3333361
GCAGGTA1400.072.59
GAGCAAA10900.072.51
TCGACTT502.2997065E-672.56
AGCAAAA11400.068.684212
CGTTACA851.6916601E-1068.23533
AGCGAAA2800.067.321432
CGACTTT554.0501236E-665.909097
TTCGACT554.0501236E-665.909095
GCGAAAG2900.065.03
CCGTTAC902.8012437E-1064.444442
CAAAAGC12300.064.247974
GCAAAAG12200.064.180333
CGAAAGC2950.063.8983044
GGAGTAC350.003562467262.1428571
GTTACAC954.5292836E-1061.0526354
GCAGGGT2750.060.6363649
TTACACC1007.1486284E-1058.05
AAAGCAG17250.056.318846
Produced by FastQC (version 0.11.2)