FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_2187_day_3_ll-e.3520000008bbea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_2187_day_3_ll-e.3520000008bbea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258191
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA29351.1367553477851668No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG23010.8912007002567865No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT20210.7827538527679121No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC18300.7087776103737156No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC14050.5441707882923882No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13790.534100723882707No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG13760.5329387933738976No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT10970.42487925605462623No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA10690.41403457130573873No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA9580.3710431424797921No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT9520.36871928146217336No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC9490.367557350953364No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA8590.3326994356890829No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG8560.33153750518027353No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG8370.32417861195781417No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT8090.3133339272089267No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT7590.29396841872877055No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG7440.28815876618472375No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA7210.2792506322838519No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG6800.2633709153301238No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA6440.2494277492244114No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT6420.24865312888520513No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA6170.23897037464512705No Hit
GAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAA6040.23393534244028646No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA6010.23277341193147708No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT5980.23161148142266771No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC5890.2281256898962396No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC5870.22735106955703333No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG5860.22696375938743024No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA5860.22696375938743024No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT5850.22657644921782713No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC5760.22309065769139902No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT5630.2180556254865584No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT5400.20914749158568655No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG5360.20759825090727407No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC5310.20566170005925846No Hit
TCATCCTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCT5170.2002393576848147No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG5070.1963662559887835No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC4980.19288046446235538No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC4850.1878454322575148No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC4630.17932460852624607No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG4490.17390226615180235No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT4490.17390226615180235No Hit
CCCCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATAT4450.17235302547338985No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA4410.17080378479497738No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG4370.16925454411656488No Hit
GGAACACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACA4340.1680926136077555No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA4310.16693068309894613No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT4300.16654337292934301No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT4180.16189565089410551No Hit
GTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA4130.1599591000460899No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG4050.15686061868926493No Hit
GGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGA3850.14911441529720246No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG3820.1479524847883931No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT3810.14756517461878996No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG3680.14253014241394937No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG3650.14136821190513998No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT3630.14059359156593376No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT3600.13943166105712437No Hit
AAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTAATGTTAAATACATA3600.13943166105712437No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA3580.13865704071791815No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG3570.138269730548315No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC3570.138269730548315No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC3490.13517124919149No Hit
CTCTATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCC3450.1336220085130775No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA3420.13246007800426815No Hit
GTTCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTG3400.1316854576650619No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC3330.12897428647784007No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3300.12781235596903068No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC3290.12742504579942754No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG3260.12626311529061818No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3240.12548849495141193No Hit
GTATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG3200.12393925427299944No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC3110.12045346274657134No Hit
GGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAG3100.12006615257696822No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT3050.11812960172895261No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAG3040.11774229155934947No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA3020.11696767122014323No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG3000.11619305088093698No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2990.11580574071133386No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT2990.11580574071133386No Hit
CTATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAA2990.11580574071133386No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC2990.11580574071133386No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT2970.11503112037212762No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC2920.11309456952411201No Hit
ATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTC2900.11231994918490575No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATT2890.11193263901530263No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC2870.11115801867609637No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2840.109996088167287No Hit
GTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTAATGTTAAATA2840.109996088167287No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT2820.10922146782808076No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT2820.10922146782808076No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT2800.10844684748887452No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA2780.10767222714966827No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG2780.10767222714966827No Hit
GGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA2780.10767222714966827No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC2760.10689760681046201No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT2750.10651029664085891No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT2730.10573567630165265No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC2700.10457374579284327No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC2690.10418643562324016No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA2690.10418643562324016No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGT2670.10341181528403393No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2670.10341181528403393No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC2650.10263719494482766No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT2630.10186257460562143No Hit
GCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGTAG2600.10070064409681205No Hit
CATCATCCTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT2590.10031333392720891No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGG100.007087837145.01
CTAGGAC100.007087837145.03
GAGCGAA3150.0131.190481
GAGCAAA12900.0129.825581
ATTAGTA700.0103.571421
GTATGAC301.5972013E-596.6666643
CTTGCCG1200.096.6666646
AGCAGGC4050.094.876548
AAGCAGG22500.093.122227
GCAGGCA4150.089.096389
GCAGGTC7250.088.09
AGCAGGT11050.085.9502268
GCAGGGT5550.084.9099049
AGCAGGG9000.083.777778
GCAGGGG3500.078.714289
GCAGGTA4150.076.867479
AGCGAAA5250.075.9523852
CGAAAGC5400.073.84264
GCGAAAG5400.073.84263
AAAGCAG28800.073.003476
Produced by FastQC (version 0.11.2)