FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_2184_day_1_ul-b.3520000008bbc0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_2184_day_1_ul-b.3520000008bbc0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246290
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG27591.1202241260302896No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA15590.6329936254009502No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC12180.4945389581387795No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA10240.41577002720370293No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG9710.3942506800925738No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG9500.3857241463315604No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT9090.3690771042267246No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG7800.3166998254090706No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT7180.29152624954322137No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT6550.2659466482601811No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC6380.2590442161679321No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC5440.2208778269519672No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC5310.2155994965284827No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA5030.20423078484713142No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA4560.18514759023914895No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG4520.18352348857038453No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA4380.1778391327297089No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT4310.17499695480937108No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT4240.17215477688903327No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG4050.16444029396240203No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA4020.1632222177108287No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG3960.16078606520768202No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC3820.15510170936700637No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG3760.15266555686385969No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA3550.14413902310284624No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT3490.14170287059969952No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT3480.14129684518250843No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG3460.1404847943481262No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG3290.13358236225587722No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG3250.13195826058711274No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT3210.13033415891834826No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT3180.12911608266677493No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA3150.12789800641520158No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA3130.12708595558081937No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG3020.12261967599171708No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT2990.12140159974014374No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA2910.1181533964026148No Hit
ATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTC2840.11531121848227699No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT2820.11449916764789476No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC2800.11368711681351253No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC2730.1108449388931747No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT2690.10922083722441024No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2690.10922083722441024No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT2670.10840878639002802No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA2660.1080027609728369No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT2660.1080027609728369No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA2610.10597263388688132No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2600.10556660846969021No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAG2590.10516058305249909No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG2520.10231840513216127No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC2520.10231840513216127No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG2510.10191237971497015No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGT2510.10191237971497015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2050.0141.463411
GAGCAAA14150.098.8869251
GCAGGGT8600.091.046519
AGTAGAA1450.090.01
GCAGGTA3600.088.6111159
AAGCAGG19350.086.1757057
AGCGAAA3450.084.057972
AGCAGGG9900.083.484858
CCTACTA353.4321238E-582.857145
CACCTAC353.4321238E-582.857143
CGAAAGC3700.078.378384
GCGAAAG3700.078.378383
AAAGCAG21600.076.863436
CAAAAGC18300.075.6693954
AGCAAAA18950.074.2216342
AGCAGGT8550.072.923988
TTATAGG406.653227E-572.499991
AGCAGGC2250.070.8888858
AAAAGCA20350.068.046685
GCAAAAG20900.066.949763
Produced by FastQC (version 0.11.2)