FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_2015_day_8_ll-e.3520000008bbf7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_2015_day_8_ll-e.3520000008bbf7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336408
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC15960.4744239138189342No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA15450.45926375115930657No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA11110.3302537395067894No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA8050.23929276354902382No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA6930.20599985731611614No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT5410.16081662742859862No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC5250.15606049796675464No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA5240.1557632398753894No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG5190.15427694941856318No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA4980.148034529499893No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT4860.14446743240351No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG4760.14149485148985755No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC4730.14060307721576182No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA4430.1316853344748044No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT4400.13079356020070868No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT4150.1233621079165775No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA4060.12068678509429026No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC3970.11801146227200304No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC3730.1108772680792371No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA3710.11028275189650663No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT3560.10582388052602792No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT3550.10552662243466268No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG3500.10404033197783645No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA3500.10404033197783645No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA3420.10166226724691446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3950.0115.632911
AGCAAAA19750.080.0253142
AAAAGCA20850.076.151085
GAGCAAA20850.075.803351
GCAAAAG21400.073.855143
AAAGCAG27850.072.6301656
GCGAAGC300.001934622172.53
CGTGAAC300.001934622172.52
GTCTCGG300.001934622172.5145
AAGGTCG502.3029097E-672.55
AGCGAAA6000.072.52
ATACCGA300.001934622172.56
GCGAAAG6250.069.63
CAAAAGC22850.069.168494
CGAAAGC6450.067.4418644
GGTCGTT554.0557516E-665.909097
CATACCG350.003564447462.1428575
CCGAAAT350.003564447462.1428579
AGCAGGT5050.057.4257478
GCAGGGG11450.055.0873349
Produced by FastQC (version 0.11.2)