FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n02_2001_day_1_ll-e.3520000008bbdd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n02_2001_day_1_ll-e.3520000008bbdd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325932
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA18190.5580918719242051No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC17020.5221948136421094No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA8350.25618840739786214No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA8190.2512794079746696No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA6660.20433710099039062No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA5210.15984929371770798No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA5110.15678116907821263No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC4940.15156535719107053No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA4690.14389504559233215No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA4640.14236098327258445No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT4610.14144054588073585No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT4470.13714517138544235No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT3990.12241817311586466No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG3980.12211136065191514No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC3970.12180454818796559No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG3950.12119092326006653No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA3870.11873642354847022No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC3780.11597511137292442No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG3730.11444104905317673No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC3730.11444104905317673No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA3670.11260017426947952No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA3610.11075929948578232No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC3510.10769117484628696No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA3460.10615711252653928No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG3420.10492986267074114No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG3270.10032767571149809No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2750.0113.363641
AGCAAAA17600.078.678982
GCAAAAG18500.074.4594653
CAAAAGC19950.069.774444
GAGCAAA20550.068.090031
GCGAAAG4700.064.787233
CGTGTTC451.1926558E-464.444446
AAAGCGT451.1926558E-464.444442
AGCGAAA4850.062.7835042
AAAAGCA22450.062.3273935
CGAAAGC4900.062.1428574
AAAGCAG27400.061.9160586
GCAGGGG9200.050.4347849
GCAGGAG7800.050.192319
GCGTGAG604.9401424E-448.3333325
AAGCAGG35750.047.6573457
AGCAGGA11100.043.761268
TCACGAT700.001055591941.428573
GAAAGCA7750.040.2258035
GGTATTC2005.456968E-1239.875145
Produced by FastQC (version 0.11.2)