FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_zymobiomics_community_standard.3510000008bdeb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_zymobiomics_community_standard.3510000008bdeb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633208
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTA3655.675247E-921.8493141
CTGAGGT2053.6643902E-421.2195134
AGGGGTG1800.004305355720.138895
TAACTAG2701.0783752E-418.7962975
GTATATA4404.8588845E-818.1251
ATACAGT5301.1768861E-917.7830186
TTAGACC2050.00912281417.6829264
AGGGCAG2050.00912281417.6829265
GTGTTAC3302.509534E-517.5757581
TAAGACT2550.001590122517.0588254
GTACTAT3002.4151102E-416.9166681
TAGAACG3052.7397924E-416.6393454
TATACAG4409.654213E-716.4772725
CCTTATA3254.444222E-415.6153862
TAGTACA2800.002969803315.5357144
ATATATG6801.9772415E-914.926471
CTATAAC3901.04803155E-414.8717953
ACTGCAG2950.004203027614.7457638
GTAGAAC2950.004203027614.7457633
TACTATG2950.004203027614.7457632
Produced by FastQC (version 0.11.2)