FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_zika-la-10000.3510000008bd9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_zika-la-10000.3510000008bd9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18028
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG2981.652984246727313No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC2871.5919680497004658No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG2831.5697803416907032No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC2741.5198579986687375No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC2711.5032172176614156No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC2631.4588418016418903No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC2581.431107166629687No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG2531.4033725316174839No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG2461.3645440426003994No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA2381.3201686265808743No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG2271.259152429554027No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA2171.2036831595296207No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA2151.1925893055247394No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC2001.1093854004881296No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA1951.0816507654759264No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC1800.9984468604393165No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG1790.9928999334368759No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC1660.9207898824051476No Hit
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC1630.9041491013978257No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG1620.898602174395385No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG1600.8875083203905035No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG1600.8875083203905035No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG1540.8542267583758598No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG1520.8431329043709785No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG1440.7987574883514533No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG1320.7321943643221654No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC1260.6989128023075216No Hit
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC1250.693365875305081No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC1180.6545373862879965No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC1180.6545373862879965No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT1140.6323496782782339No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG1060.5879742622587086No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC1050.582427335256268No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC1010.5602396272465054No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT950.5269580652318615No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC950.5269580652318615No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT940.5214111382294209No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC920.5103172842245396No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG920.5103172842245396No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC910.504770357222099No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC900.49922343021965826No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG890.4936765032172176No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT890.4936765032172176No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT880.48812957621477704No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG880.48812957621477704No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT860.47703572220989576No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC860.47703572220989576No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA850.471488795207455No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG850.471488795207455No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC820.45484801420013315No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC790.4382072331928112No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT790.4382072331928112No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG770.4271133791879299No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG770.4271133791879299No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT770.4271133791879299No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG760.42156645218548927No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG760.42156645218548927No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT730.4049256711781673No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC730.4049256711781673No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT720.39937874417572666No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA710.393831817173286No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG700.38828489017084533No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT700.38828489017084533No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG700.38828489017084533No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG690.3827379631684047No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC690.3827379631684047No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG690.3827379631684047No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG670.3716441091635234No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG660.3660971821610827No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT660.3660971821610827No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA660.3660971821610827No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC650.3605502551586421No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC650.3605502551586421No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG640.3550033281562015No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC630.3494564011537608No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG630.3494564011537608No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC630.3494564011537608No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG620.34390947415132017No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC620.34390947415132017No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT620.34390947415132017No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT610.33836254714887953No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC610.33836254714887953No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC610.33836254714887953No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG610.33836254714887953No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT610.33836254714887953No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG600.33281562014643884No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC600.33281562014643884No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG590.32726869314399826No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC580.32172176614155756No Hit
CGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA580.32172176614155756No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG580.32172176614155756No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG580.32172176614155756No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA560.3106279121366763No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA560.3106279121366763No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT560.3106279121366763No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA560.3106279121366763No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC550.30508098513423565No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG540.299534058131795No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC520.2884402041269137No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGAC510.28289327712447304No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACA510.28289327712447304No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC500.2773463501220324No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC500.2773463501220324No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT490.27179942311959177No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC480.2662524961171511No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG470.26070556911471043No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG470.26070556911471043No Hit
CAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTT460.2551586421122698No Hit
CAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT450.24961171510982913No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG450.24961171510982913No Hit
CAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTT430.23851786110494788No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC420.2329709341025072No Hit
ACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGT420.2329709341025072No Hit
ACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGT420.2329709341025072No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGAC420.2329709341025072No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC410.22742400710006658No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC410.22742400710006658No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG410.22742400710006658No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTT400.22187708009762588No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC400.22187708009762588No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACA400.22187708009762588No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC400.22187708009762588No Hit
ACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGT390.21633015309518527No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC390.21633015309518527No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTG380.21078322609274464No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC380.21078322609274464No Hit
CAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTT380.21078322609274464No Hit
CAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTT340.18859551808298203No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC320.17750166407810075No Hit
ACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGT310.17195473707566009No Hit
CGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA300.16640781007321942No Hit
CAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTT300.16640781007321942No Hit
CGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACA290.16086088307077878No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG290.16086088307077878No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTG280.15531395606833814No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTG260.14422010206345684No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTG240.13312624805857556No Hit
ACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGT240.13312624805857556No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTG230.1275793210561349No Hit
CGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACA230.1275793210561349No Hit
ACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGT190.10539161304637232No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTG190.10539161304637232No Hit
GGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACT190.10539161304637232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTGC38350.010.3976545
GTTCAGA38650.010.3169471
CAGACTG38750.010.2903224
TTCAGAC39050.010.2112672
TCAGACT38550.010.15564253
AAAAAAA6652.2292697E-67.631579145
ACTGCGA82600.06.1440687
TGCGACA82650.06.14035139
CTGCGAC83950.06.04526478
GACTGCG83400.05.99820146
CTGAACT26703.0108326E-55.1591763
TGAACTG26703.0108326E-55.1591764
TCTGAAC27003.627089E-55.1018522
Produced by FastQC (version 0.11.2)