FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_zika-la-1000.3510000008bd8e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_zika-la-1000.3510000008bd8e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95911
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC21042.193700409754877No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG20342.120716080532994No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC18901.9705768889908355No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG17401.8141818978010864No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG15791.646317940590756No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC12801.3345705914858568No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG12351.287652094128932No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG11951.245946763144999No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG11581.2073693319848608No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC10481.092679671779045No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA10361.0801680724838651No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA10061.0488890742459156No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC9621.0030132101635891No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG9040.9425404802368862No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC8870.9248157145687148No Hit
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC8840.9216878147449198No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC8490.8851956501339784No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG8400.8758119506625934No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG8180.8528740186214303No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG8060.8403624193262503No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC8020.8361918862278571No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT7900.8236802869326771No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA7800.813253954186694No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC7700.8028276214407106No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG7650.7976144550677191No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC7640.7965718217931208No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC7620.7944865552439241No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG7250.7559091240837861No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA7030.7329711920426228No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC6670.6954363941570831No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG6540.6818821615873049No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG6510.67875426176351No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC6470.6745837286651166No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG6460.6735410953905183No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC6120.6380915640541752No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC6030.6287078645827903No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC5920.6172388985622087No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC5730.5974288663448405No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC5670.5911730666972506No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG5570.5807467339512673No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA5450.5682351346560874No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG5320.5546809020863092No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC5300.5525956355371126No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG5270.5494677357133175No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG5190.541126669516531No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC5180.5400840362419326No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG5110.5327856033197443No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG5100.531742970045146No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT5000.5213166372991628No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC4920.5129755711023761No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG4920.5129755711023761No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG4830.5035918716309912No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG4810.5015066050817946No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG4660.4858671059628197No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG4560.4754407732168365No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG4530.47231287339304145No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG4440.46292917392165656No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC4440.46292917392165656No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG4050.4222664762123219No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT4020.4191385763885269No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT3780.3941153777981671No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT3730.3889022114251754No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG3570.3722200790316022No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG3570.3722200790316022No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG3530.36804954593320893No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA3450.35970847973642234No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG3430.3576232131872257No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT3320.3461542471666441No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT3280.34198371406825073No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG3280.34198371406825073No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC3280.34198371406825073No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA3270.34094108079365243No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT3160.32947211477307087No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT3160.32947211477307087No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG3140.3273868482238742No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC3130.3263442149492759No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA3080.3211310485762843No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT2960.30861944928110435No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC2890.3013210163589161No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT2890.3013210163589161No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC2880.30027838308431776No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC2880.30027838308431776No Hit
CGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA2850.29715048326052274No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG2730.28463888396534287No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC2730.28463888396534287No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT2720.2835962506907445No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC2720.2835962506907445No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT2690.28046835086694955No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC2610.27212728467016295No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA2470.2575304188257864No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC2370.24710408607980316No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC2320.2418909197068115No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT2310.2408482864322132No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC2300.23980565315761485No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG2210.23042195368622995No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT2190.22833668713703328No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG2170.22625142058783662No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT2110.21999562094024672No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT2100.21895298766564836No Hit
CAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT2070.21582508784185336No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC2030.21165455474346007No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC1990.2074840216450668No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC1990.2074840216450668No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA1980.20644138837046846No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC1940.20227085527207514No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC1900.19810032217368184No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTG1890.19705768889908354No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG1870.19497242234988688No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC1860.19392978907528854No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACA1860.19392978907528854No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTT1800.18767398942769858No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGAC1770.18454608960390362No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACA1770.18454608960390362No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTG1750.18246082305470696No Hit
CAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTT1720.179332923230912No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG1720.179332923230912No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG1580.16473605738653543No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC1560.1626507908373388No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC1450.1511818248167572No Hit
ACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGT1450.1511818248167572No Hit
AACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG1430.14909655826756055No Hit
CGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA1430.14909655826756055No Hit
CGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACA1410.14701129171836388No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC1410.14701129171836388No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGAC1390.14492602516916725No Hit
CAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTT1350.14075549207077395No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC1320.13762759224697899No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC1310.13658495897238063No Hit
CAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC1300.13554232569778232No Hit
CCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAA1290.134499692423184No Hit
CAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTT1250.1303291593247907No Hit
CGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACA1240.12928652605019236No Hit
CAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTT1230.12824389277559403No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTG1180.1230307264026024No Hit
ACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGT1180.1230307264026024No Hit
CAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTT1160.12094545985340575No Hit
GTCACAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC1140.11886019330420912No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGC100.0070809405145.03
ATCTAGG100.0070809405145.02
AGGCTAC100.0070809405145.06
AGGTTCT100.0070809405145.06
TGTCGCC2051.2745071E-524.756098145
AAAACAC852.1736567E-413.6470585135-139
CACAGTC7501.5150908E-611.63
TCACAGT7551.6413596E-611.5231782
ACAGTCC8203.5309677E-711.4939024
GTCACAG7651.923061E-611.3725491
TCAGACT138900.010.0737943
AGACTGC140700.010.0479755
CAGACTG142200.010.0439534
GTTCAGA141150.010.0159411
TTCAGAC141350.09.9504782
AAAAACA3409.827179E-66.8235292135-139
AAAAATA2700.00151402996.444444130-134
TGCGACA355600.06.01448259
TCGTATG19406.537309E-55.9793816145
ACTGCGA358800.05.9406357
Produced by FastQC (version 0.11.2)