FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_zika-la-100.3510000008bd71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_zika-la-100.3510000008bd71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114067
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG11651.021329569463561No Hit
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC10720.9397985394548818No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG9010.7898866455679557No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG8630.7565728913708609No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC8620.7556962136288322No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA7990.7004655158810172No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTC7880.6908220607187004No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG7680.6732885058781243No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG7360.6452348181332024No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG7300.6399747516810296No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA6900.6049076419998772No Hit
GTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA6280.5505536219940912No Hit
GTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA5990.5251299674752558No Hit
GTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC5950.5216232565071406No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCG5830.5111031236027949No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC5640.4944462465042475No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC5560.48743282456801706No Hit
AGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC5540.4856794690839594No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC5490.4812960803738154No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC5430.4760360139216426No Hit
AGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC5370.4707759474694697No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTG5230.45850245908106646No Hit
AGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTC5190.4549957481129512No Hit
GTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC5140.45061235940280714No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG4860.4260653826260005No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTC4850.4251887048839717No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG4810.4216819939158564No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCG4750.4164219274636836No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGC4580.40151840584919396No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT4520.39625833939702104No Hit
GTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGC4490.39362830617093464No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG4450.3901215952028194No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG4350.3813548177825313No Hit
GTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGC4280.37521807358832965No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTG4250.37258804036224324No Hit
GACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTG4030.35330113003760943No Hit
AGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTC3910.3427809971332638No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG3840.33664425293906214No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG3830.3357675751970333No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT3610.31648066487239956No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG3580.31385063164631316No Hit
GTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG3570.31297395390428434No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCG3530.30946724293616906No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCA3440.30157714325790985No Hit
GACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTG3410.2989471100318234No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG3360.29456372132167935No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTG3330.29193368809559295No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC3210.2814135551912472No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCG3150.2761534887390744No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTG3110.2726467777709592No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC3050.26738671131878633No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCG3050.26738671131878633No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC3050.26738671131878633No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG3040.2665100335767575No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCC3040.2665100335767575No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC2980.26124996712458465No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCG2860.250729834220239No Hit
AGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT2850.2498531564782102No Hit
GTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTG2840.24897647873618137No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCG2790.24459309002603735No Hit
GTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG2780.24371641228400853No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC2720.2384563458318357No Hit
GTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTG2680.23494963486372045No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC2630.2305662461535764No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT2620.22968956841154758No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT2590.22705953518546118No Hit
GTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCA2530.22179946873328835No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT2430.21303269131300026No Hit
AGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT2360.2068959471187986No Hit
GAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT2350.2060192693767698No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACT2310.20251255840865456No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCA2290.20075920292459698No Hit
AACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTG2290.20075920292459698No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCG2230.1954991364724241No Hit
GTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCA2210.19374578098836648No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCT2180.19111574776228005No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCC2070.18147229259996317No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC2050.17971893711590556No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCT2040.17884225937387677No Hit
CAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTC2010.17621222614779034No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCC2000.17533554840576152No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACT1970.1727055151796751No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTC1960.1718288374376463No Hit
CTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG1920.16832212646953107No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCC1910.16744544872750225No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACT1910.16744544872750225No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTC1870.16393873775938703No Hit
GAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACT1820.15955534904924298No Hit
GCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGAC1750.15341860485504133No Hit
AGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCT1690.1481585384028685No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC1690.1481585384028685No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC1680.14728186066083968No Hit
CAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT1660.14552850517678206No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG1660.14552850517678206No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACA1660.14552850517678206No Hit
CTGCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG1640.14377514969272445No Hit
CGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA1640.14377514969272445No Hit
AGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCT1620.14202179420866684No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCG1600.1402684387246092No Hit
CGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACA1550.1358850500144652No Hit
GCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGAC1520.13325501678837876No Hit
CAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTC1510.13237833904634994No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC1510.13237833904634994No Hit
CTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTC1480.12974830582026353No Hit
CAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTC1430.1253649171101195No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTC1430.1253649171101195No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCG1410.12361156162606188No Hit
GTCACAGTCCGAACTGTCGCAGTCCGAACTGTCGCAGTCCGAACTGTCGC1390.12185820614200427No Hit
CTGTCGCAGTCTGAACTGTCGCAGTCCGAACTGTCGCAGTCTGAACTGTC1330.1165981396898314No Hit
GCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGAC1310.11484478420577379No Hit
CAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCGGACTGCGACAGTT1300.113968106463745No Hit
CTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCG1290.11309142872171618No Hit
GCGACAGTTCGGACTGCGACAGTTCAGACTGCGACAGTTCAGACTGCGAC1280.11221475097968737No Hit
CGACAGTTCAGACTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACA1190.10432465130142811No Hit
CTGCGACAGTTCAGACTGCGACAGTTCGGACTGCGACAGTTCAGACTGCG1180.1034479735593993No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCG151.2271719E-4145.05
TTCCGTG151.2271719E-4145.07
CTTCCGT203.8571848E-4108.756
ACTACGA300.001931332472.52
GACTACG300.001931332472.51
CTACGAC350.003558409662.1428533
ATAGGAC502.0026675E-458.03
GGCCCGT502.0026675E-458.06
GACATGG502.0026675E-458.07
TAGGCCC502.0026675E-458.04
TAGGACA502.0026675E-458.04
CTAGGCC502.0026675E-458.03
GCCCGTC502.0026675E-458.07
TACGACA400.006037204554.3754
GATAGAA400.006037204554.3751
TATAGGA553.2069045E-452.7272762
ATATAGG604.926551E-448.3333321
ACATGGG657.3091703E-444.6153878
ACCGTAT700.001052704941.42857145
CCCGTCC750.001477876638.6666648
Produced by FastQC (version 0.11.2)