FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_zika-la-10.3510000008bd64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_zika-la-10.3510000008bd64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308664
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC6110.19794987429697014No Hit
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA5670.18369489153254023No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT4670.15129720343156314No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT4510.14611357333540678No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC4510.14611357333540678No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC4120.1334784749760257No Hit
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC4080.13218256745198662No Hit
CCCTAATAGTGGCCATCATTTTGCTCGTGGCGCACTACATGTACTTGATC3430.11112407018635151No Hit
CAGCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTG3390.10982816266231242No Hit
GTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTG3350.10853225513827335No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA3100.10043283311302906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGAAT100.007088505144.999981
GTCATAA702.8048817E-972.499991
TAATACT754.840331E-967.6666645
ATAATAC902.0432708E-856.3888854
TCATAAT902.0432708E-856.3888852
AATACTG902.0432708E-856.3888856
TCTTCCG1550.051.451615
CACCGAA1255.176844E-946.3999989
ACACCGA1307.3214323E-944.6153878
TATAGGA2850.043.2456132
ATATAGG2900.042.51
CTTCCGT1751.8189894E-1241.428576
CTACCAC700.001055505241.428573
TTCCGTG1803.8198777E-1140.2777757
GAATCTA951.02724E-438.157895
AATCTAT951.02724E-438.157896
ACCGTAT3150.034.52381145
CGATAGA1707.7929144E-834.117645145
TCCTATA3000.033.8333322
CCAAAAT1402.740579E-531.0714262
Produced by FastQC (version 0.11.2)