FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_zika-her-reg.3510000008bdb4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_zika-her-reg.3510000008bdb4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360419
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC34470.9563868719462626No Hit
GTCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT29870.8287576404129637No Hit
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA28270.7843648642274686No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC25880.7180531548003851No Hit
GTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTG23170.6428628901362027No Hit
CCCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA21960.6092908531459218No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC21130.5862621004996962No Hit
GTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC19180.532158404523624No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAA15820.438933574534084No Hit
TCCCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTG14810.4109106345669901No Hit
GTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT14580.4045291729903252No Hit
TCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTC13770.38205533004641823No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC12650.3509803867165715No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGG11370.3154661657681754No Hit
TACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGT11210.31102688814962587No Hit
CCCACACCTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA11110.3082523396380324No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC9530.2644144731548559No Hit
GTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGATTGCTTGTCAA9300.2580330115781909No Hit
CAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAAT9260.2569231921735535No Hit
GTGTATTCTCTTGATTCCAACCAGGTTTGCGACCGCGTTTGCAGCTTCCT9050.2510966402992073No Hit
AGTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCT9000.2497093660434106No Hit
TCCGTACACAACCCAAGTTGACGTCGTGTTGCACCAACAATCGACGTCAT8810.24443772387138302No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG8760.2430504496155863No Hit
GTGTTGGGCAGCGGCTGTCAGAAGCCATGTCTGATATTGATGCCTCATAG8730.24221808506210826No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA8660.24027590110399286No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC8430.23389443952732794No Hit
TGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGC8390.23278462012269055No Hit
GTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTC8330.23111989101573444No Hit
CCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGAT8250.22890025220645968No Hit
CATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGG8150.22612570369486626No Hit
GCTTCCTGGTGGAATGGGAGGGGAGCGTCACAGCTCTTCTAGATCTCCGT8030.2227962454809541No Hit
GTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGA7930.22002169696936066No Hit
ATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCCCC7600.21086568688110227No Hit
GTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACT7450.2067038641137121No Hit
GTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTTG7440.20642640926255276No Hit
GTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAG7430.20614895441139341No Hit
CTCAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGA7320.20309695104864062No Hit
GAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGA7280.20198713164400325No Hit
CATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGG7220.20032240253704714No Hit
GAATAAGTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCA7180.19921258313240978No Hit
ATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCA7020.19477330551386027No Hit
TCTTTATTGTTTCCATAGCCTCTTTTTTCCCCACTGAACCCCATCTATTG7000.19421839581154157No Hit
CACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGATTGCT6990.19394094096038222No Hit
ATGCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAG6780.18811438908603598No Hit
TCCTAGCATTGATTATTCTCAGCATGGCAGCCAGATCTTTCTTGAACTTC6750.18728202453255793No Hit
CTTCTAGATCTCCGTGCTTCACCTTTTTTGTGATGGCAGGTTCCGTACAC6730.18672711483023924No Hit
ATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGA6570.18228783721168973No Hit
GGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAG6470.17951328870009628No Hit
GTGTACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCTAGAAG6430.1784034692954589No Hit
ATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCAT6410.17784855959314022No Hit
TACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCC6400.17757110474198087No Hit
GCATAGGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACT6390.17729364989082153No Hit
GTCAATATGCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTT6210.1722994625699533No Hit
TCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTC6130.17007982376067854No Hit
CTATTGATGAGACCCAGTGATGGCTTGATTGCCGTGAATCTCAAAAAGGC6070.16841509465372248No Hit
GAATAATCAATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTAGT5860.16258854277937623No Hit
ATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACCAGGAAGCTGCAAA5820.16147872337473884No Hit
TCCCCAATGTGGTTGGAAAAGATATGGCCTCCCCAGCATCGTTTCTGTCC5640.15648453605387064No Hit
CAATCAAGTCCTAGGCTTCCAAAACCCCCCAGGGTGGCTTCGGCTCTCGG5640.15648453605387064No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC5580.15481980694691458No Hit
CACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCCCCAGCATC5570.15454235209575523No Hit
GTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTGT5540.1537099875422772No Hit
AGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAG5500.1526001681376398No Hit
CTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTTTT5500.1526001681376398No Hit
CTAGTATCTGCGCCTCGTCTCTTCTTCTCCTTCCTAGCATTGATTATTCT5470.15176780358416175No Hit
GCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGC5360.14871580022140898No Hit
TCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTAT5340.1481608905190903No Hit
CAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAAAGA5110.14177942894242535No Hit
TAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCAG5020.13928233528199124No Hit
TTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGCTTGGCTTTT5000.13872742557967255No Hit
CTAATCAAGTGCTTTGTGTATTCTCTTGATTCCAACCAGGTTTGCGACCG4990.1384499707285132No Hit
ATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGCAA4970.1378950610261945No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC4910.13623033191923845No Hit
ATCCAGCATAGGGCATTCATAGCTCATGGTGGCATCACACATGTGTCCAA4900.1359528770680791No Hit
GATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACCAGGAAGCTGCAA4870.13512051251460105No Hit
ATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTAGTGTCGGAATT4860.1348430576634417No Hit
GACATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTG4830.1340106931099637No Hit
GCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCT4790.1329008737053263No Hit
TCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAAAG4780.13262341885416695No Hit
TCACACATGTGTCCAAGATCCATGATCTGTATATAACACTTATTCATCCC4740.1315135994495296No Hit
GGGTATGGCTATTGGGTTCATGCCACAGATGGTCATCAGGACCACCTTGA4740.1315135994495296No Hit
GTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCAGCA4730.13123614459837024No Hit
ATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTAT4730.13123614459837024No Hit
CATCAGGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCA4700.1304037800448922No Hit
CACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATG4640.1287390509379361No Hit
CTCTTGATTCCAACCAGGTTTGCGACCGCGTTTGCAGCTTCCTGGTGGAA4630.12846159608677676No Hit
ATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTG4580.12707432183098005No Hit
GTACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCTAGAAGAG4460.12374486361706792No Hit
GGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCA4460.12374486361706792No Hit
AGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTTGGATTT4380.12152522480779315No Hit
ACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTG4290.11902813114735905No Hit
GTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGG4280.1187506762961997No Hit
GGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAA4250.11791831174272167No Hit
GGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACACGACGT4240.11764085689156233No Hit
CAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCAT4220.11708594718924364No Hit
CTGTACCTCCACTGTGACTGTCCCGTGCAGTGTTTCAGCCGGGATCTTGG4210.1168084923380843No Hit
GGAGTTACAGTACGCAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGA4200.11653103748692495No Hit
CCTTTTTTGTGATGGCAGGTTCCGTACACAACCCAAGTTGACGTCGTGTT4190.1162535826357656No Hit
GCTAGCAACAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTT4190.1162535826357656No Hit
AATCAAGTGCTTTGTGTATTCTCTTGATTCCAACCAGGTTTGCGACCGCG4190.1162535826357656No Hit
GCATACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTT4140.11486630837996886No Hit
TCCCCACACCGTTTCTGTCCAAGTACATATAGTATGCACTCCCACGTCTA4110.11403394382649083No Hit
GGTTTGCGACCGCGTTTGCAGCTTCCTGGTGGAATGGGAGGGGAGCGTCA4100.11375648897533149No Hit
GGCATACAGCATCAGGTGCATAGGAGTCAGCAATAGGGACTTTGTGGAAG4080.11320157927301279No Hit
ATGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGT4030.11181430501721608No Hit
ATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAAGTGT3990.1107044856125787No Hit
GCAGTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAA3940.10931721135678198No Hit
GTCCAAGTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCA3930.10903975650562263No Hit
CATGTGTCCAAGATCCATGATCTGTATATAACACTTATTCATCCCCAATG3930.10903975650562263No Hit
TGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGGTG3900.10820739195214457No Hit
GCCATAGCTGTGGTCAGCAGGAGGCCAACAATTCCGACACTAGTATCTGC3890.10792993710098524No Hit
GATATGGCCTCCCCAGCATCGTTTCTGTCCAAGTACATATAGTATGCACT3880.10765248224982589No Hit
GATCTGTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGG3870.10737502739866654No Hit
GATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAG3830.10626520799402916No Hit
ATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCA3820.10598775314286982No Hit
CATTGGGGATGAATAAGTGTTATATACAGATCATGGATCTTGGACACATG3790.10515538858939179No Hit
GAGCTATGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCG3780.10487793373823244No Hit
CCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGG3770.1046004788870731No Hit
TATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCC3730.10349065948243573No Hit
GAGAATAATCAATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTA3720.10321320463127638No Hit
GTACCTCCACTGTGACTGTCCCGTGCAGTGTTTCAGCCGGGATCTTGGTG3670.10182593037547966No Hit
ATTCCGGATTGTCAATATGCTAAAACGCGGAGTAGCCCGTGTGAGCCCCT3660.10154847552432031No Hit
GATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCC3640.10099356582200163No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAAT100.007088993145.00002145
TTAACGT100.007088993145.000023
CCCCAAC600.0132.916671
AACCTCT550.0118.636365
ACAGTTC501.8189894E-12116.03
CACGTTT203.8640283E-4108.750011
CAGTTCG553.6379788E-12105.4545444
ACCTCTG2150.097.7906956
GTCATAA6450.091.046511
ACACCTC1700.089.558824
CACGAAA502.2644599E-887.01
ACGAAAG502.2644599E-887.02
GTTCGAG1600.086.093761
CACACCT1800.084.5833363
TTCGAGT1650.083.484852
CGAGTTT1650.083.484854
TCGAGTT1650.083.484853
CCAACCT801.8189894E-1281.562513
ACCCATT451.2312812E-680.555567
TCATAAT7800.075.288462
Produced by FastQC (version 0.11.2)