FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_zika-her-dmso.3510000008bdde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_zika-her-dmso.3510000008bdde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453650
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC41220.9086300011021713No Hit
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA33110.7298578199052133No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC30320.668356662625372No Hit
GTCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT29870.6584371211286234No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC25540.5629890885043536No Hit
GTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTG24280.5352143723134575No Hit
GTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC22320.4920092582387303No Hit
CCCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA20100.4430728535214372No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAA16850.37143172048936407No Hit
GTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT16370.3608508762261656No Hit
TCCCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTG15370.33880745067783535No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC15200.3350600683346192No Hit
TCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTC14650.3229361842830376No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGG13100.2887688746831258No Hit
TACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGT12380.27289760828832804No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC11950.263418935302546No Hit
CCCACACCTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA11420.251735919761931No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG10910.2404937727322826No Hit
CTCAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGA10330.22770858591425108No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC10200.22484294059296814No Hit
TCCGTACACAACCCAAGTTGACGTCGTGTTGCACCAACAATCGACGTCAT10050.2215364267607186No Hit
GTGTATTCTCTTGATTCCAACCAGGTTTGCGACCGCGTTTGCAGCTTCCT10030.221095558249752No Hit
GTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGATTGCTTGTCAA9890.21800947867298578No Hit
GTGTTGGGCAGCGGCTGTCAGAAGCCATGTCTGATATTGATGCCTCATAG9800.21602557037363607No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA9790.21580513611815277No Hit
CATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGG9790.21580513611815277No Hit
ATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCCCC9530.2100738454755869No Hit
TGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGC9440.2080899371762372No Hit
AGTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCT9300.20500385759947096No Hit
GAATAAGTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCA9190.20257908078915463No Hit
GTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTTG9160.20191777802270472No Hit
GTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGA9060.1997134354678717No Hit
GTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTC9060.1997134354678717No Hit
CAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAAT8960.1975090929130387No Hit
CATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGG8810.19420257908078917No Hit
CCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGAT8660.1908960652485396No Hit
TACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCC8390.18494434035049048No Hit
GTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAG8200.18075608949630773No Hit
CTTCTAGATCTCCGTGCTTCACCTTTTTTGTGATGGCAGGTTCCGTACAC8040.17722914140857488No Hit
GTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACT7710.1699548109776259No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC7640.1684117711892428No Hit
ATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCA7600.1675300341673096No Hit
TCCCCAATGTGGTTGGAAAAGATATGGCCTCCCCAGCATCGTTTCTGTCC7460.16444395459054337No Hit
ATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGA7460.16444395459054337No Hit
CAATCAAGTCCTAGGCTTCCAAAACCCCCCAGGGTGGCTTCGGCTCTCGG7450.16422352033506005No Hit
GTCAATATGCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTT7370.16246004629119365No Hit
GCTTCCTGGTGGAATGGGAGGGGAGCGTCACAGCTCTTCTAGATCTCCGT7350.16201917778022706No Hit
ATGCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAG7290.16069657224732722No Hit
GAATAATCAATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTAGT7290.16069657224732722No Hit
GTGTACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCTAGAAG7200.15871266394797753No Hit
ATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCAT7170.15805136118152763No Hit
CACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGATTGCT7080.15606745288217788No Hit
GGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAG7070.1558470186266946No Hit
GCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGC7050.15540615011572798No Hit
GCATAGGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACT7040.15518571586024468No Hit
CTAATCAAGTGCTTTGTGTATTCTCTTGATTCCAACCAGGTTTGCGACCG7020.15474484734927807No Hit
TCCTAGCATTGATTATTCTCAGCATGGCAGCCAGATCTTTCTTGAACTTC7010.15452441309379478No Hit
ATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACCAGGAAGCTGCAAA6940.15298137330541167No Hit
TCTTTATTGTTTCCATAGCCTCTTTTTTCCCCACTGAACCCCATCTATTG6780.14945442521767882No Hit
TCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTC6650.1465887798963959No Hit
CACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCCCCAGCATC6610.1457070428744627No Hit
GAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGA6570.14482530585252948No Hit
ATCCAGCATAGGGCATTCATAGCTCATGGTGGCATCACACATGTGTCCAA6510.14350270031962967No Hit
GTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGG6430.14173922627576324No Hit
TCACACATGTGTCCAAGATCCATGATCTGTATATAACACTTATTCATCCC6310.13909401520996362No Hit
TTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGCTTGGCTTTT6170.1360079356331974No Hit
TCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTAT6170.1360079356331974No Hit
AGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAG6010.13248098754546456No Hit
GATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACCAGGAAGCTGCAA5980.13181968477901468No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC5970.13159925052353136No Hit
GGGTATGGCTATTGGGTTCATGCCACAGATGGTCATCAGGACCACCTTGA5900.13005621073514825No Hit
CTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTTTT5860.12917447371321503No Hit
CAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAAAGA5850.12895403945773173No Hit
CTAGTATCTGCGCCTCGTCTCTTCTTCTCCTTCCTAGCATTGATTATTCT5830.12851317094676512No Hit
ATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCA5790.12763143392483192No Hit
ATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGCAA5720.12608839413644882No Hit
CATCAGGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCA5680.1252066571145156No Hit
CTATTGATGAGACCCAGTGATGGCTTGATTGCCGTGAATCTCAAAAAGGC5670.1249862228590323No Hit
CAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCAT5630.12410448583709907No Hit
GGAGTTACAGTACGCAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGA5610.12366361732613247No Hit
ATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTG5600.12344318307064917No Hit
GCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCT5590.12322274881516589No Hit
GTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTGT5500.12123884051581615No Hit
TCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAAAG5490.12101840626033286No Hit
GACATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTG5490.12101840626033286No Hit
GGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACACGACGT5470.12057753774936625No Hit
CTCTTGATTCCAACCAGGTTTGCGACCGCGTTTGCAGCTTCCTGGTGGAA5380.11859362945001654No Hit
ATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTAT5350.11793232668356664No Hit
CACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATG5340.11771189242808333No Hit
AGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTTGGATTT5330.11749145817260002No Hit
GATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAG5300.11683015540615012No Hit
TAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCAG5300.11683015540615012No Hit
GGCATACAGCATCAGGTGCATAGGAGTCAGCAATAGGGACTTTGTGGAAG5220.1150666813622837No Hit
ATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAAGTGT5200.11462581285131708No Hit
TTCCAAGACAACATCAACCCAAGTCCCACCTGACATACCTTCCACAAAGT5180.1141849443403505No Hit
GGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAGCCA5170.1139645100848672No Hit
TGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGGTG5150.11352364157390059No Hit
GTACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCTAGAAGAG5150.11352364157390059No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC5130.11308277306293399No Hit
GCTAGCAACAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTT5050.11131929901906756No Hit
GATCTGTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGG5040.11109886476358426No Hit
GTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGTGGTCAGCA5000.11021712774165104No Hit
CTGTACCTCCACTGTGACTGTCCCGTGCAGTGTTTCAGCCGGGATCTTGG4930.10867408795326794No Hit
CTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTG4910.10823321944230134No Hit
ATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTAGTGTCGGAATT4910.10823321944230134No Hit
ATGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGT4910.10823321944230134No Hit
CATTGGGGATGAATAAGTGTTATATACAGATCATGGATCTTGGACACATG4890.10779235093133473No Hit
GATATGGCCTCCCCAGCATCGTTTCTGTCCAAGTACATATAGTATGCACT4870.10735148242036813No Hit
GGTTTGCGACCGCGTTTGCAGCTTCCTGGTGGAATGGGAGGGGAGCGTCA4870.10735148242036813No Hit
AATCAAGTGCTTTGTGTATTCTCTTGATTCCAACCAGGTTTGCGACCGCG4820.10624931114295161No Hit
GGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAA4740.10448583709908521No Hit
GCAGTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAA4730.1042654028436019No Hit
GCATACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTT4720.1040449685881186No Hit
GAGCTATGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCG4650.10250192879973548No Hit
TCCCCACACTTGTCTAGGATTGGAGATCCTGAAGTTCCTGCTGGGTAATC4640.10228149454425217No Hit
GTACCTCCACTGTGACTGTCCCGTGCAGTGTTTCAGCCGGGATCTTGGTG4630.10206106028876887No Hit
ATGTTTGAGTGGGCATTCCTTCAGTGTGTCACCATCCACGACAAAGCTGT4610.10162019177780227No Hit
GCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATAT4550.10029758624490247No Hit
CCCTAGAACCACGACAGTTTGCCTTTTGGCATGTGCGTCCTTGAACTCTA4550.10029758624490247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATGT100.0070895934145.01
GCGACAG202.12934E-6145.01
ACACCCA100.0070895934145.04
ATCACGT100.0070895934145.02
CCATCGT100.0070895934145.01
AACCTCT400.0145.05
GGTTATA100.0070895934145.01
CCCCAAC553.6379788E-12105.4545441
ACCTCTG1600.099.68756
ACTTGTC1050.089.761918
ACACTTG1050.089.761916
CACACTT1050.089.761915
CACTTGT1150.088.260877
ACACTGT502.2659151E-887.06
CACCCAG259.383418E-487.01
GTCATAA6500.084.769231
TCGAGTT1800.084.5833363
TTAAGAA353.4352488E-582.857144
CGAGTTT1850.082.29734
ACACCTC1250.081.24
Produced by FastQC (version 0.11.2)