FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_zika-her-be.3510000008bdc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_zika-her-be.3510000008bdc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507356
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC32860.6476714575170098No Hit
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA21690.42751046602385706No Hit
GTCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT21580.42534236315328877No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC19680.38789331357074713No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC17010.33526754389422814No Hit
GTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC16690.32896033554348425No Hit
CCCCAGCCTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA13610.26825345516757465No Hit
CCCCACACTCTGTCCACTAACGTTCTTTTGCAGACATATTGAGTGTCTGA13050.2572158405537729No Hit
GAGTGTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCTGGTGACA12600.24834632881053934No Hit
GGGAAATCCATGGGTTCGGGGTATTTCACACTAGTGTCTGGCTCAAGGTT12580.24795212828861787No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC11990.23632321289193386No Hit
GTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT11500.22666530010485736No Hit
TGATCCTCCACAAGAAAGCTGTTCCATGCTCTATGTTTGAGTGGGCATTC11150.21976679097123122No Hit
GTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTG11000.21681028705682007No Hit
TCCCCACACTTGTCTAGGATTGGAGATCCTGAAGTTCCTGCTGGGTAATC10910.21503638470817335No Hit
TCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTC10780.21247408131568365No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC10580.20853207609646873No Hit
GAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCTGGTGACA10370.2043929706162931No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCTTCACCTTGTGTTGGG9300.18330324269349332No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAA9120.1797554379961999No Hit
CTTCTATTCCATCTGTCCACAATGTGTGGGACTTTGGCCATTCACATGTT8730.17206852781873083No Hit
GATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAG8340.16438161764126175No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG8340.16438161764126175No Hit
GTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGG8000.15768020876859642No Hit
TCCGTACACAACCCAAGTTGACGTCGTGTTGCACCAACAATCGACGTCAT8000.15768020876859642No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC7720.15216140146169554No Hit
GTGTATTCTCTTGATTCCAACCAGGTTTGCGACCGCGTTTGCAGCTTCCT7660.15097879989593105No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC7550.14881069702536287No Hit
CTCAATATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGA7530.14841649650344138No Hit
ATGTTTGAGTGGGCATTCCTTCAGTGTGTCACCATCCACGACAAAGCTGT7420.14624839363287317No Hit
GGAGTTACAGTACGCAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGA7420.14624839363287317No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC7340.1446715915451872No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA7220.14230638841365825No Hit
CTGTACCTCCACTGTGACTGTCCCGTGCAGTGTTTCAGCCGGGATCTTGG6880.1356049795409929No Hit
CTTCTAGATCTCCGTGCTTCACCTTTTTTGTGATGGCAGGTTCCGTACAC6470.12752386884160236No Hit
ATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCCCC6410.1263412672758379No Hit
GTACCTCCACTGTGACTGTCCCGTGCAGTGTTTCAGCCGGGATCTTGGTG6200.1222021617956622No Hit
GAATAAGTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCA6130.120822459968937No Hit
GTGTTGGGCAGCGGCTGTCAGAAGCCATGTCTGATATTGATGCCTCATAG6090.12003405892509401No Hit
ATTCTCATCAGTTTCATGTCCTGTGTCATTAACGATCATCCCACTGTGCT6040.11904855762029029No Hit
TGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGC5990.11806305631548657No Hit
GTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAG5810.11451525161819313No Hit
GTAGAAGGGGAGCTCAACGCAATCCTGGAAGAGAATGGAGTTCAACTGAC5720.11274134926954643No Hit
CATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGG5720.11274134926954643No Hit
CTCTGGTATTGTGATGGCTGAGTGGCCCAGCTAAAGACTTGGGTATGATC5710.11254424900858569No Hit
CCCTAGAACCACGACAGTTTGCCTTTTGGCATGTGCGTCCTTGAACTCTA5710.11254424900858569No Hit
GGGTATGGCTATTGGGTTCATGCCACAGATGGTCATCAGGACCACCTTGA5700.11234714874762494No Hit
TACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTGT5650.11136164744282122No Hit
CTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTG5590.11017904587705674No Hit
ATCTTAGAGTTCTCAGTGCTTTCAGTGATTACGGGGTTAGCGGTTATCAA5380.1060399403968811No Hit
CTCTTACATTGTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGC5340.1052515393530381No Hit
GGGGAAATCCATGGGTTCGGGGTATTTCACACTAGTGTCTGGCTCAAGGT5230.10308343648246991No Hit
ACTCTGTACACTCCATCTGTGGTCTCCCCCTTTTTTACTTCCTTGGGAGC5180.10209793517766619No Hit
CAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAATCAAT5160.10170373465574467No Hit
GTGTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCTGGTGACATG5150.10150663439478394No Hit
TACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCC5100.10052113308998022No Hit
ATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGA5080.10012693256805873No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGG100.0070898393145.000023
AAAAGCG100.0070898393145.000023
TACCGGG100.0070898393145.000022
ACTGCGC203.8649468E-4108.750011
GTCATAA4350.081.666671
CGAGTTT903.6379788E-1272.54
TCGAGTT903.6379788E-1272.53
ACACTTG2650.065.660386
CACTTGT2650.065.660387
TTCGAGT1051.6370905E-1162.1428572
ACTTGTC3000.060.4166648
GTTCGAG1102.5465852E-1159.318181
GTATCAT8700.058.3333361
ACAGTTC502.0092961E-458.04
GCGACAG502.0092961E-458.01
CAGTTCG502.0092961E-458.05
CACACTT3050.057.049185
TCATAAT6250.056.842
TATCATG9000.056.388892
ACCAAGT13600.055.9742669
Produced by FastQC (version 0.11.2)