FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_negative_rt-pcr_control_l_cb7-9_2.3510000008bca9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_negative_rt-pcr_control_l_cb7-9_2.3510000008bca9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATTGTGTGAACCCGGGAGACAGAGGTTGTGGTGAGCCGAGATTGT151.0148849797023005No Hit
GTGTGAACCCGGGAGACAGAGGTTGTGGTGAGCCGAGATTGTGCCATTGC90.6089309878213802No Hit
CCTCTAGCCCCACCCGCTGTCCCCTGCTCCTCATGCCCCCATGCTGTGTC80.5412719891745602No Hit
CAGTAGGACCAGATCATGTAAGGAAAATACTGCAGGCTCATCTTGGGGCT70.4736129905277402No Hit
ATCCCAAGGTGATCCCTCACAGGAAGTGACTCAGAAAAGCAGTGAGCTAC70.4736129905277402No Hit
AGCATATCCTGAGCCAGGCTGACCTTAAAAAGTAAGCATTCAGGGCCTAG60.40595399188092013No Hit
ACCCACTCCTAGCAAAGGGCTCAGCAGCACACGGCCTCCCTGCGGGGATG60.40595399188092013No Hit
AGACAGAGGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCTGGG50.3382949932341001No Hit
GGGTCCTACTAAGAGCCAGGACTGAGGAGCAAGGCCCAAAAAACTCCTCT50.3382949932341001No Hit
GCAGCACACGGCCTCCCTGCGGGGATGCCCTCGGGGAAGATGTGGCCCAG50.3382949932341001No Hit
AGCGAAAGCAGGATAATTGTGTGAACCCGGGAGACAGAGGTTGTGGTGAG50.3382949932341001No Hit
GCCCACAGTAGGACCAGATCATGTAAGGAAAATACTGCAGGCTCATCTTG40.2706359945872801No Hit
GGTGATCCCTCACAGGAAGTGACTCAGAAAAGCAGTGAGCTACGAAAGCT40.2706359945872801No Hit
ACTGTACCTGCTGCACCTCATCTGACACCAGCTTCTCTGCTTTGCCCAAG40.2706359945872801No Hit
GGCTCATCTTGGGGCTGCACTGGCGACCTGAGAACACCTTATCCTACTGT40.2706359945872801No Hit
GGGCCTAGGGACCTTGGGCAAAGCAGAGAAGCTGGTGTCAGATGAGGTGC40.2706359945872801No Hit
CTTTTTAAGGTCAGCCTGGCTCAGGATATGCTAGTTTGGCTGCAGAGGGT40.2706359945872801No Hit
GTCCTACTAAGAGCCAGGACTGAGGAGCAAGGCCCAAAAAACTCCTCTGG40.2706359945872801No Hit
AGCCAGGACTGAGGAGCAAGGCCCAAAAAACTCCTCTGGGCCACATCTTC40.2706359945872801No Hit
AAGAAATTCCATCCCAAGGTGATCCCTCACAGGAAGTGACTCAGAAAAGC40.2706359945872801No Hit
TCTTGGGGCTGCACTGGCGACCTGAGAACACCTTATCCTACTGTACCTGC40.2706359945872801No Hit
ACTAAGAGCCAGGACTGAGGAGCAAGGCCCAAAAAACTCCTCTGGGCCAC40.2706359945872801No Hit
GAAAAGCAGTGAGCTACGAAAGCTATCTGAATGAAGCAAGATCGTCATGT40.2706359945872801No Hit
CAGGATAATTGTGTGAACCCGGGAGACAGAGGTTGTGGTGAGCCGAGATT40.2706359945872801No Hit
AGCGAAAGCAGGAGGATCACTTGAGGCCAAGAGTTCAAGACCAGCCTAGG40.2706359945872801No Hit
GCAGAGGCCTCCCTAAACCTCCCTCTCCTCTAGCCCCACCCGCTGTCCCC30.20297699594046006No Hit
ACCCGGGAGACAGAGGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCA30.20297699594046006No Hit
GACTACCCACTCCTAGCAAAGGGCTCAGCAGCACACGGCCTCCCTGCGGG30.20297699594046006No Hit
GACAGAGGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCTGGGT30.20297699594046006No Hit
GCGAAAGCAGAGGCCTCCCTAAACCTCCCTCTCCTCTAGCCCCACCCGCT30.20297699594046006No Hit
GCCCCAAGATGAGCCTGCAGTATTTTCCTTACATGATCTGGTCCTACTGT30.20297699594046006No Hit
CTTCTCTGCTTTGCCCAAGGTCCCTAGGCCCTGAATGCTTACTTTTTAAG30.20297699594046006No Hit
GTCTCCAAATAAAAAAAAAAAAGGAAAAGAAATTCCATCCCAAGGTGATC30.20297699594046006No Hit
CAGGAAGTGACTCAGAAAAGCAGTGAGCTACGAAAGCTATCTGAATGAAG30.20297699594046006No Hit
GCTTTCGTAGCTCACTGCTTTTCTGAGTCACTTCCTGTGAGGGATCACCT30.20297699594046006No Hit
GGCTCAGGATATGCTAGTTTGGCTGCAGAGGGTCCTACTAAGAGCCAGGA30.20297699594046006No Hit
CCTCATCTGACACCAGCTTCTCTGCTTTGCCCAAGGTCCCTAGGCCCTGA30.20297699594046006No Hit
CCTCAGTCCTGGCTCTTAGTAGGACCCTCTGCAGCCAAACTAGCATATCC30.20297699594046006No Hit
GCCCAAGGTCCCTAGGCCCTGAATGCTTACTTTTTAAGGTCAGCCTGGCT30.20297699594046006No Hit
TCTGACACCAGCTTCTCTGCTTTGCCCAAGGTCCCTAGGCCCTGAATGCT30.20297699594046006No Hit
GTACCTGCTGCACCTCATCTGACACCAGCTTCTCTGCTTTGCCCAAGGTC30.20297699594046006No Hit
TCATGTGGGAGACACTGACTACCCACTCCTAGCAAAGGGCTCAGCAGCAC30.20297699594046006No Hit
GTGACTCAGAAAAGCAGTGAGCTACGAAAGCTATCTGAATGAAGCAAGAT30.20297699594046006No Hit
GCTCAGGATATGCTAGTTTGGCTGCAGAGGGTCCTACTAAGAGCCAGGAC30.20297699594046006No Hit
ATCCTACTGTACCTGCTGCACCTCATCTGACACCAGCTTCTCTGCTTTGC30.20297699594046006No Hit
GGCCTCACTTCCGTTGCCATGTTTTCATAATCCTCTCAGGCTCTGGGCAG30.20297699594046006No Hit
GTCTCGCACTGTCACCCAGGCTGGAGTGCAATGGCACAATCTCGGCTCAC30.20297699594046006No Hit
CCCTAAACCTCCCTCTCCTCTAGCCCCACCCGCTGTCCCCTGCTCCTCAT30.20297699594046006No Hit
TCTCTGCTTTGCCCAAGGTCCCTAGGCCCTGAATGCTTACTTTTTAAGGT30.20297699594046006No Hit
TAATTGTGTGAACCCGGGAGACAGAGGTTGTGGTGAGCCGAGATTGTGCC30.20297699594046006No Hit
CTCAGGCTCTGGGCAGCAGCGCTGCCCACAGTAGGACCAGATCATGTAAG20.13531799729364005No Hit
GAATGAAGCAAGATCGTCATGTGGGAGACACTGACTACCCACTCCTAGCA20.13531799729364005No Hit
ATCTTGCTTCATTCAGATAGCTTTCGTAGCTCACTGCTTTTCTGAGTCAC20.13531799729364005No Hit
GTAGAAACAAGGTCTCACCATATTGCCTAGGCTGGTCTTGAACTCTTGGC20.13531799729364005No Hit
TTTAAGGTCAGCCTGGCTCAGGATATGCTAGTTTGGCTGCAGAGGGTCCT20.13531799729364005No Hit
CCTTGGGATGGAATTTCTTTTCCTTTTTTTTTTTATTTGGAGACAGAGTC20.13531799729364005No Hit
GGCTGCAGAGGGTCCTACTAAGAGCCAGGACTGAGGAGCAAGGCCCAAAA20.13531799729364005No Hit
CCCTCACAGGAAGTGACTCAGAAAAGCAGTGAGCTACGAAAGCTATCTGA20.13531799729364005No Hit
ATCTGGTCCTACTGTGGGCAGCGCTGCTGCCCAGAGCCTGAGAGGATTAT20.13531799729364005No Hit
AATCCTCTCAGGCTCTGGGCAGCAGCGCTGCCCACAGTAGGACCAGATCA20.13531799729364005No Hit
GTCCCCTGGCCTCACTTCCGTTGCCATGTTTTCATAATCCTCTCAGGCTC20.13531799729364005No Hit
CTCCTCATGCCCCCATGCTGTGTCCCCTGGCCTCACTTCCGTTGCCATGT20.13531799729364005No Hit
TAGCAAAGGGCTCAGCAGCACACGGCCTCCCTGCGGGGATGCCCTCGGGG20.13531799729364005No Hit
GCTTCATTCAGATAGCTTTCGTAGCTCACTGCTTTTCTGAGTCACTTCCT20.13531799729364005No Hit
ACGAAAGCTATCTGAATGAAGCAAGATCGTCATGTGGGAGACACTGACTA20.13531799729364005No Hit
CCCTAGGCCCTGAATGCTTACTTTTTAAGGTCAGCCTGGCTCAGGATATG20.13531799729364005No Hit
AGCGAAAGCAGAGGCCTCCCTAAACCTCCCTCTCCTCTAGCCCCACCCGC20.13531799729364005No Hit
GGCTCTGGGCAGCAGCGCTGCCCACAGTAGGACCAGATCATGTAAGGAAA20.13531799729364005No Hit
CCTTATCCTACTGTACCTGCTGCACCTCATCTGACACCAGCTTCTCTGCT20.13531799729364005No Hit
AGCAGAGGCCTCCCTAAACCTCCCTCTCCTCTAGCCCCACCCGCTGTCCC20.13531799729364005No Hit
CTGTGTCCCCTGGCCTCACTTCCGTTGCCATGTTTTCATAATCCTCTCAG20.13531799729364005No Hit
CCTCTGGGCCACATCTTCCCCGAGGGCATCCCCGCAGGGAGGCCGTGTGC20.13531799729364005No Hit
CACTCCAGCCTGGGTGACAGTGCGAGACTCTGTCTCCAAATAAAAAAAAA20.13531799729364005No Hit
CTGGGTGACAGTGCGAGACTCTGTCTCCAAATAAAAAAAAAAAAGGAAAA20.13531799729364005No Hit
GTCGGCGCGCGCATCGAGACCGGCGGCGAGCGGATCGGCTCGGAAGGCAA20.13531799729364005No Hit
CAGCTTCTCTGCTTTGCCCAAGGTCCCTAGGCCCTGAATGCTTACTTTTT20.13531799729364005No Hit
AGCGAAAGCAGGACCCCTTCTGCCTTGTTTCTACTCTGTCTCTTATACAC20.13531799729364005No Hit
GCTAGGAGTGGGTAGTCAGTGTCTCCCACATGACGATCTTGCTTCATTCA20.13531799729364005No Hit
CCCCCATGCTGTGTCCCCTGGCCTCACTTCCGTTGCCATGTTTTCATAAT20.13531799729364005No Hit
ATCTTGGGGCTGCACTGGCGACCTGAGAACACCTTATCCTACTGTACCTG20.13531799729364005No Hit
ATCTGAATGAAGCAAGATCGTCATGTGGGAGACACTGACTACCCACTCCT20.13531799729364005No Hit
GCCCTGAATGCTTACTTTTTAAGGTCAGCCTGGCTCAGGATATGCTAGTT20.13531799729364005No Hit
ACTCCAGCCTGGGTGACAGTGCGAGACTCTGTCTCCAAATAAAAAAAAAA20.13531799729364005No Hit
ATGCTAGTTTGGCTGCAGAGGGTCCTACTAAGAGCCAGGACTGAGGAGCA20.13531799729364005No Hit
AGCGAAAGCAGGGATCAAATGGTTTTCCTCCTTCAAGCCTTGTTTCTACT20.13531799729364005No Hit
CTCCAGCCTGGGTGACAGTGCGAGACTCTGTCTCCAAATAAAAAAAAAAA20.13531799729364005No Hit
CAAAAGCAGGGATCAAATGGTTTTCCTCCTTCAAGCCTTGTTTCTACTCT20.13531799729364005No Hit
TTGCTAGGAGTGGGTAGTCAGTGTCTCCCACATGACGATCTTGCTTCATT20.13531799729364005No Hit
GAGCGAAAGCAGGAGGATCACTTGAGGCCAAGAGTTCAAGACCAGCCTAG20.13531799729364005No Hit
ATCCTGAGCCAGGCTGACCTTAAAAAGTAAGCATTCAGGGCCTAGGGACC20.13531799729364005No Hit
TCCCAAGGTGATCCCTCACAGGAAGTGACTCAGAAAAGCAGTGAGCTACG20.13531799729364005No Hit
GTGGGTAGTCAGTGTCTCCCACATGACGATCTTGCTTCATTCAGATAGCT20.13531799729364005No Hit
TCCCTAGGCCCTGAATGCTTACTTTTTAAGGTCAGCCTGGCTCAGGATAT20.13531799729364005No Hit
GCCTGGCTCAGGATATGCTAGTTTGGCTGCAGAGGGTCCTACTAAGAGCC20.13531799729364005No Hit
GGACTGAGGAGCAAGGCCCAAAAAACTCCTCTGGGCCACATCTTCCCCGA20.13531799729364005No Hit
GTTCTCAGGTCGCCAGTGCAGCCCCAAGATGAGCCTGCAGTATTTTCCTT20.13531799729364005No Hit
GTCCTGGCTCTTAGTAGGACCCTCTGCAGCCAAACTAGCATATCCTGAGC20.13531799729364005No Hit
CAGAAAAGCAGTGAGCTACGAAAGCTATCTGAATGAAGCAAGATCGTCAT20.13531799729364005No Hit
AGAAATTCCATCCCAAGGTGATCCCTCACAGGAAGTGACTCAGAAAAGCA20.13531799729364005No Hit
CCCCTGCTCCTCATGCCCCCATGCTGTGTCCCCTGGCCTCACTTCCGTTG20.13531799729364005No Hit
ATGTGGGAGACACTGACTACCCACTCCTAGCAAAGGGCTCAGCAGCACAC20.13531799729364005No Hit
CACACGGCCTCCCTGCGGGGATGCCCTCGGGGAAGATGTGGCCCAGAGGA20.13531799729364005No Hit
AGGTGATCCCTCACAGGAAGTGACTCAGAAAAGCAGTGAGCTACGAAAGC20.13531799729364005No Hit
GTTTTCATAATCCTCTCAGGCTCTGGGCAGCAGCGCTGCCCACAGTAGGA20.13531799729364005No Hit
CCTGCACGCGATCCAGGACGACGCGGGCTACGTGCCGTCGGGCTGCGTCG20.13531799729364005No Hit
GGGAGACACTGACTACCCACTCCTAGCAAAGGGCTCAGCAGCACACGGCC20.13531799729364005No Hit
CCCTGCGGGGATGCCCTCGGGGAAGATGTGGCCCAGAGGAGTTTTTTGGG20.13531799729364005No Hit
TCGTAGCTCACTGCTTTTCTGAGTCACTTCCTGTGAGGGATCACCTTGGG20.13531799729364005No Hit
GTCTCCCACATGACGATCTTGCTTCATTCAGATAGCTTTCGTAGCTCACT20.13531799729364005No Hit
GCTGCACCTCATCTGACACCAGCTTCTCTGCTTTGCCCAAGGTCCCTAGG20.13531799729364005No Hit
GCACTGGCGACCTGAGAACACCTTATCCTACTGTACCTGCTGCACCTCAT20.13531799729364005No Hit
GGGCTGCACTGGCGACCTGAGAACACCTTATCCTACTGTACCTGCTGCAC20.13531799729364005No Hit
CTACGAAAGCTATCTGAATGAAGCAAGATCGTCATGTGGGAGACACTGAC20.13531799729364005No Hit
ATATGCTAGTTTGGCTGCAGAGGGTCCTACTAAGAGCCAGGACTGAGGAG20.13531799729364005No Hit
GGAAAATACTGCAGGCTCATCTTGGGGCTGCACTGGCGACCTGAGAACAC20.13531799729364005No Hit
GAGCTACGAAAGCTATCTGAATGAAGCAAGATCGTCATGTGGGAGACACT20.13531799729364005No Hit
GGTCCCTAGGCCCTGAATGCTTACTTTTTAAGGTCAGCCTGGCTCAGGAT20.13531799729364005No Hit
GGGTAGTCAGTGTCTCCCACATGACGATCTTGCTTCATTCAGATAGCTTT20.13531799729364005No Hit
AAGCAAGATCGTCATGTGGGAGACACTGACTACCCACTCCTAGCAAAGGG20.13531799729364005No Hit
GCCGAGATTGTGCCATTGCACTCCAGCCTGGGTGACAGTGCGAGACTCTG20.13531799729364005No Hit
ATCTTCCCCGAGGGCATCCCCGCAGGGAGGCCGTGTGCTGCTGAGCCCTT20.13531799729364005No Hit
GAGGAGCAAGGCCCAAAAAACTCCTCTGGGCCACATCTTCCCCGAGGGCA20.13531799729364005No Hit
GTCCCTAGGCCCTGAATGCTTACTTTTTAAGGTCAGCCTGGCTCAGGATA20.13531799729364005No Hit
GGCCACATCTTCCCCGAGGGCATCCCCGCAGGGAGGCCGTGTGCTGCTGA20.13531799729364005No Hit
ATGTGGCCCAGAGGAGTTTTTTGGGCCTTGCTCCTCAGTCCTGGCTCTTA20.13531799729364005No Hit
GAACTGATGGCCGCGTATTACGCGCAGCGCGCGAGTGCGGGCCTGATCGT20.13531799729364005No Hit
AGCAAAGGGCTCAGCAGCACACGGCCTCCCTGCGGGGATGCCCTCGGGGA20.13531799729364005No Hit
ACCTTGGGCAAAGCAGAGAAGCTGGTGTCAGATGAGGTGCAGCAGGTACA20.13531799729364005No Hit
AGTAGAAACAAGGTCTCACCATATTGCCTAGGCTGGTCTTGAACTCTTGG20.13531799729364005No Hit
GAGGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCTGGGTGACA20.13531799729364005No Hit
GGTTGTGGTGAGCCGAGATTGTGCCATTGCACTCCAGCCTGGGTGACAGT20.13531799729364005No Hit
GCACATCACGCGGATCGTCACCGACAACGGCCAAACGGTCGTCGACGACG20.13531799729364005No Hit
AAACTAGCATATCCTGAGCCAGGCTGACCTTAAAAAGTAAGCATTCAGGG20.13531799729364005No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATCC100.0063819443145.0145
Produced by FastQC (version 0.11.2)