FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_library_prep_negative_control_3.3510000008be13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_library_prep_negative_control_3.3510000008be13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG70.26789131266743205No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT40.15308075009567548No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGCAG100.0066919946145.06
CCGGTTC100.0066919946145.09
AAGCAGG100.0066919946145.07
Produced by FastQC (version 0.11.2)