FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_hrv14-5c-c3-4_52c.3510000008bd3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_hrv14-5c-c3-4_52c.3510000008bd3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386802
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT13500.3490157755130532No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG11110.2872270567370386No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC10310.26654464041033915No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT9840.2543937208184032No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT8320.21509712979767426No Hit
GGCTAGGACACGGTCACCCGTGGGGAATTCCTAGCCTCATCGACCAAACT7840.2026876800016546No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT7680.19855119673631472No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA7370.19053676040971865No Hit
CTGGTACTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTT7240.18717586775663No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA6930.17916143143003396No Hit
CTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTTAAAATT6740.17424935755244286No Hit
ATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTTAAAATTCC6620.17114699510343792No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT6140.15873754530741827No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG6130.15847901510333454No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG6080.1571863640829158No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT6000.15511812245024587No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC5620.1452939746950636No Hit
TCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAA5600.14477691428689615No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC5480.1416745518378912No Hit
TATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGG5160.13340158530721144No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA5060.130816283266374No Hit
ACCTAAAAGAGGTCCAACCAGCGCCTCCTAAGTACGCTGTATTCTGGGAG5000.12926510204187155No Hit
GGTATAGGCTGTACCCACTGCCAAAAGCCTTTAACCGTTATCCGCCAACC4910.12693833020511786No Hit
TTCATATTGTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGA4730.12228478653161048No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG4510.11659712204176813No Hit
ATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAA4380.11323622938867947No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA4160.10754856489883713No Hit
CAGTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATG4130.1067729742865859No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG4100.10599738367433467No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG4050.10470473265391596No Hit
TATTAATACTATTCTGATGTTTGTCAGTACATTAGGTATTGTTTATGTCA4030.10418767224574846No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT3980.10289502122532974No Hit
ATCCAATGGTGTCTATGTACAAGCACTTCTGTTTCCCCGGAGCGAGGTAT3950.10211943061307852No Hit
CTCTGGTACTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCC3920.1013438400008273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCGT100.0070891934145.02
GAATCTC100.0070891934145.05
TACGAAC100.0070891934145.03
GTATACG100.0070891934145.01
GACATAT151.2294269E-4145.02
CTCCTAT100.0070891934145.01
AACTATG100.0070891934145.01
ACATATG151.2294269E-4145.03
TGTCGTG100.0070891934145.03
TCACGTG100.0070891934145.01
CTCTGGT458.367351E-11112.777771
CATATGT203.8642454E-4108.754
CTGGTAC1450.095.01
GGACATA259.3823654E-487.01
TCCTACT554.3872205E-879.0909042
CTCCTAC651.5606929E-978.076921
CTACTTC651.3957833E-766.923074
CCGTCGC2250.064.44444145
ACTATGT2150.064.069766
TACTTCT702.3307621E-762.1428575
Produced by FastQC (version 0.11.2)