FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_hrv14-5c-c3-1_57c.3510000008bd58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_hrv14-5c-c3-1_57c.3510000008bd58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491729
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT12070.24546040603665842No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT9960.20255059189106195No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG9870.2007203154583114No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG8700.17692672183255412No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC8690.1767233577844707No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT8500.1728594408708862No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG8130.16533497109180056No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT8010.1628946025147998No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA7700.15659031702421455No Hit
GGCTTGAAGTATCTCTTTAAAAATGTCAAGTTTTCCCATGTCATTTTTGT7130.144998566283461No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT6970.1417447415141267No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA6750.13727073245629198No Hit
TCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAA6740.1370673684082086No Hit
GTATTCTGGGAGAATTAGACATTTTCCAATGTCAGTAGTTCTGATCTTCT6080.12364534123470447No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA6050.1230352490904543No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC5540.11266368263820113No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG5510.11205359049395094No Hit
GGCTAGGACACGGTCACCCGTGGGGAATTCCTAGCCTCATCGACCAAACT5490.11164686239778414No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC5460.11103677025353395No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG5340.10859640167653323No Hit
GAGTATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTC5060.10290220833019813No Hit
GGACAATTCTTGACCACAGATGACAGGCAATCCCCCAGTGCACTGCCAAA5050.10269884428211473No Hit
CATATATTTCATCCCTAAAGATATGATGGGTATTACAAATTGATTGAATT4960.10086856784936417No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCC151.2296278E-4145.000023
AGACTAA100.007089774145.01
GGGGAGG100.007089774145.01
TCGCTAA100.007089774145.04
CTCTGAG100.007089774145.03
CCGAATG100.007089774145.04
CCGAACC100.007089774145.04
ATCGCTA100.007089774145.03
TAGTCTT100.007089774145.02
GTAGTCT100.007089774145.01
CGAAGTG100.007089774145.05
GACTAAT100.007089774145.02
TTCCGAA203.8648755E-4108.752
AAGAGGT406.112823E-790.6256
AGAGGTC406.112823E-790.6257
CGGATGG406.659822E-572.52
GCGGATG406.659822E-572.51
GAGGTCC554.0585383E-665.909098
GGTATCC553.2173912E-452.7272728
CTTCCCT450.00963624648.3333324
Produced by FastQC (version 0.11.2)