FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_hrv14-5c-c3-1_52c.3510000008bd21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_hrv14-5c-c3-1_52c.3510000008bd21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428776
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT12460.2905946228333675No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG10660.24861466126835458No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT9160.2136313599641771No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT8640.20150381551206223No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC8460.19730581935556096No Hit
GTATTCTGGGAGAATTAGACATTTTCCAATGTCAGTAGTTCTGATCTTCT8270.19287460119036512No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG7010.16348862809485606No Hit
GGCTTGAAGTATCTCTTTAAAAATGTCAAGTTTTCCCATGTCATTTTTGT6910.1611564080079109No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG6800.1585909659122712No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT6700.15625874582532603No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT6250.1457637554340728No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA6250.1457637554340728No Hit
GTACCCATTGATTCAATGACACGTCACAACAATGTCTCACTGATGGTCAT6140.14319831333843314No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA5980.13946676119932086No Hit
GGACAATTCTTGACCACAGATGACAGGCAATCCCCCAGTGCACTGCCAAA5900.13760098512976474No Hit
TCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAA5630.1313039908950128No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG5140.11987611246898147No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG5120.11940966845159244No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC5020.11707744836464727No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC4860.11334589622553502No Hit
GAGTATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTC4770.11124689814728436No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA4730.1103140101125063No Hit
ATATAAAGGAATAGATTTAGACAAACTTAAAATCTTAGCTTACGGTGATG4580.10681567998208855No Hit
CATATATTTCATCCCTAAAGATATGATGGGTATTACAAATTGATTGAATT4520.10541634792992145No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG4420.10308412784297627No Hit
GGCTAGGACACGGTCACCCGTGGGGAATTCCTAGCCTCATCGACCAAACT4320.10075190775603111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCT100.0070894593145.01
CCTAGTA100.0070894593145.05
GCGCGTG100.0070894593145.05
TCTTCCC151.2295193E-4145.04
CTCCCGT100.0070894593145.01
GCTGCGC100.0070894593145.02
CTCCACC100.0070894593145.04
CTGCGCG100.0070894593145.03
GCCTGTA100.0070894593145.04
TGCGCGT100.0070894593145.04
AAACCAC100.0070894593145.01
GGTGCAT100.0070894593145.06
GAGTTAC100.0070894593145.01
GAACCCT100.0070894593145.01
TAGTGCC151.2295193E-4145.04
GGCTGCG100.0070894593145.01
CTAATGG100.0070894593145.03
GGCTATA100.0070894593145.01
GCGGATG301.1006341E-7120.8333361
ACCTAAA203.864534E-4108.751
Produced by FastQC (version 0.11.2)