FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_hrv14-5c-c2-4_52c.3510000008bd14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_hrv14-5c-c2-4_52c.3510000008bd14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474016
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT22970.4845827988928643No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG20370.4297323297103895No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC17540.3700297036386957No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT15920.33585364207115376No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA12050.25421082832646996No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT10760.2269965570782421No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA10410.2196128400729089No Hit
GGCTAGGACACGGTCACCCGTGGGGAATTCCTAGCCTCATCGACCAAACT10090.21286201309660435No Hit
CTGGTACTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTT9700.20463444271923312No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT9660.20379058934719504No Hit
CTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTTAAAATT9620.20294673597515697No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT9120.19239856882468104No Hit
ATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCCTTAAAATTCC8850.18670255856342402No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT8730.1841709984473098No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG8550.18037365827313848No Hit
GGTATAGGCTGTACCCACTGCCAAAAGCCTTTAACCGTTATCCGCCAACC8470.1786859515290623No Hit
ATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAA7840.16539526091946263No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG7740.16328562748936745No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC7480.15780058057111995No Hit
TATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGG7270.15337035036792007No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC6720.14176736650239655No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG6620.13965773307230137No Hit
CAGTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATG6410.13522750286910146No Hit
ATCCAATGGTGTCTATGTACAAGCACTTCTGTTTCCCCGGAGCGAGGTAT6200.13079727266590158No Hit
TCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAA6050.1276328225207588No Hit
CATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGATCT5740.12109295888746371No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT5710.12046006885843516No Hit
CTCTGGTACTATGTACCTTTGTACGCCTGTTTCTCCCCAACCACCCTTCC5700.12024910551542564No Hit
TACATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA5640.11898332545736852No Hit
ACCTAAAAGAGGTCCAACCAGCGCCTCCTAAGTACGCTGTATTCTGGGAG5540.11687369202727334No Hit
TTCATATTGTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGA5520.11645176534125429No Hit
GTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC5460.1151859852831972No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT5430.11455309525416864No Hit
ACCCAAACCTGTGATGTAATCACTCAGCCCCTGTTCCTCTGCGATACACT5240.11054479173698778No Hit
TTTAAGGACATGGTGTGAAGACTCGCATGTGCTTGGTTGTGAGTCCTCCG5230.11033382839397826No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG5230.11033382839397826No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG5200.10970093836494971No Hit
GTGTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCA5110.10780226827786403No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG5080.10716937824883548No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC5050.10653648821980692No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA5040.1063255248767974No Hit
GATGTATACTGGTCCTGCCTTGTCCAGTGCTAAACTCATTCTAGCATACA5030.10611456153378789No Hit
GTATACATCAAAGAGAATCTAAGTGATCCAGACCAATGTGTATAGTACTG4980.1050597448187403No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA4920.10379396476068317No Hit
GTTATAAATTACTATAAGGATGCAGCAAGTACATCATCAGCTGGTCAATC4830.10189529467359752No Hit
TATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA4780.10084047795854993No Hit
CCCTTAAGCATGAGATCTTTAACTGGTTCTGTAAACTTAGATGGGTCCAT4770.10062951461554041No Hit
CTATCACTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGC4760.1004185512725309No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGGA100.0070896936145.02
TTCCGAA151.2296003E-4145.02
CTAGTAG100.0070896936145.03
AACTGAC100.0070896936145.02
TCTCCGG100.0070896936145.01
TAGGAGT100.0070896936145.04
CTCCTAC1100.0112.045451
TCCTACT1150.0107.173922
CTACTTC1200.0102.7083364
CTCTGGT750.096.6666641
CCGGATC259.38368E-487.04
TACTTCT1450.085.05
CTGGTAC1600.077.031251
CTTCTAC1850.070.540547
GATATAG1500.067.6666641
TTCTACT1950.066.923088
CGTTGTG1500.062.8333329
TCCGGAT350.003565346462.1428573
TAGACGT1550.060.8064545
GACGTTG1550.060.8064547
Produced by FastQC (version 0.11.2)