FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_hrv14-5c-c2-1_57c.3510000008bd4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_hrv14-5c-c2-1_57c.3510000008bd4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526791
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT13820.26234313038757306No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG11110.21089957877032825No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT11090.21051992156282093No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC9620.18261511681103132No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG9430.17900837333971156No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT9180.17426265824586978No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT9010.1710355719820574No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG8610.16344242783191057No Hit
GGCTTGAAGTATCTCTTTAAAAATGTCAAGTTTTCCCATGTCATTTTTGT8220.15603911228551742No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA7900.14996459696539993No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT7740.1469273393053412No Hit
TCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAA7380.14009350957020905No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA7360.13971385236270173No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA7290.13838505213642602No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC6530.12395807825114705No Hit
GTATTCTGGGAGAATTAGACATTTTCCAATGTCAGTAGTTCTGATCTTCT6500.12338859243988604No Hit
GGACAATTCTTGACCACAGATGACAGGCAATCCCCCAGTGCACTGCCAAA6460.12262927802487135No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC6340.12035133477982729No Hit
GAGTATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTC6090.11560561968598551No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG5840.11085990459214375No Hit
GTACCCATTGATTCAATGACACGTCACAACAATGTCTCACTGATGGTCAT5750.10915144715836073No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG5590.10611418949830198No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG5520.10478538927202628No Hit
GGCTAGGACACGGTCACCCGTGGGGAATTCCTAGCCTCATCGACCAAACT5450.1034565890457506No Hit
TATTAATACTATTCTGATGTTTGTCAGTACATTAGGTATTGTTTATGTCA5410.10269727463073591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATGG306.493792E-10145.000022
GCGGATG306.493792E-10145.000021
TCCGGAA100.0070899166145.03
GTCATGA100.0070899166145.02
AGGTATC100.0070899166145.07
TTCTACC100.0070899166145.03
AGACTAG100.0070899166145.01
TGCCCAC100.0070899166145.03
CATATTA100.0070899166145.05
CATATGT100.0070899166145.05
GCTATCC100.0070899166145.06
TCCTCAT100.0070899166145.04
CCACTTA100.0070899166145.04
ACCTAAA202.1296619E-6145.01
TACGATG100.0070899166145.03
GGTATCT100.0070899166145.08
GTTCCGG100.0070899166145.01
CCCCATT100.0070899166145.02
GTGGTTC100.0070899166145.08
TTCGATT100.0070899166145.02
Produced by FastQC (version 0.11.2)