FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_hrv14-5c-c2-1_52c.3510000008bd07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_hrv14-5c-c2-1_52c.3510000008bd07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506728
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT14200.2802292354083453No Hit
GTATATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAG13060.25773195876288657No Hit
ATGTATATGTATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC11240.22181525394294374No Hit
CATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT10130.19991001089341817No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT9300.1835304147392684No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT8760.17287381001247218No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG8120.16024375996589887No Hit
GTATTCTGGGAGAATTAGACATTTTCCAATGTCAGTAGTTCTGATCTTCT8090.15965172636996575No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT7900.1559021802623893No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA7850.15491545760250075No Hit
GGCTTGAAGTATCTCTTTAAAAATGTCAAGTTTTCCCATGTCATTTTTGT7500.14800839898328097No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG7350.14504823100361536No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA7220.14248275208790515No Hit
TCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAA6850.13518100440472997No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC6370.12570846686979997No Hit
GGACAATTCTTGACCACAGATGACAGGCAATCCCCCAGTGCACTGCCAAA6270.12373502155002289No Hit
GGCTAGGACACGGTCACCCGTGGGGAATTCCTAGCCTCATCGACCAAACT5920.11682796293080312No Hit
GAGTATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTC5920.11682796293080312No Hit
GTACCCATTGATTCAATGACACGTCACAACAATGTCTCACTGATGGTCAT5870.11584124027091457No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA5790.11426248401509291No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG5770.1138677949511375No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC5630.11110497150344958No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG5550.10952621524762793No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA5380.10617135820400689No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG5310.10478994648016292No Hit
CATATATTTCATCCCTAAAGATATGATGGGTATTACAAATTGATTGAATT5090.10044836677665335No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAA100.007089838145.03
CGAAAGC100.007089838145.03
ACTGACT100.007089838145.05
ACACCGA100.007089838145.03
GCCGGCC100.007089838145.03
ATTCGAA100.007089838145.02
CTGACTC100.007089838145.06
AAAACAG100.007089838145.05
AGTGCCG100.007089838145.07
TACGCCA100.007089838145.03
GCCGAAA100.007089838145.01
CTGCCGG100.007089838145.01
AAACCGC151.2296502E-4145.01
TCCAATT100.007089838145.03
AGTTCCA100.007089838145.01
TCTTTAC100.007089838145.01
CCGAAAG100.007089838145.02
GCTACTA100.007089838145.01
CGCACCC151.2296502E-4145.05
GACTGAC100.007089838145.04
Produced by FastQC (version 0.11.2)