FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_2427_day_5_ul-b.3510000008bc4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_2427_day_5_ul-b.3510000008bc4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286866
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA16260.56681516805756No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC13990.48768414521065584No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA6980.2433191803838726No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA6180.2154315952395892No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG5170.18022351899493141No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA4630.16139939902254014No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA4500.15686766643659408No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC3890.13560338276407802No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC3780.13176883980673904No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT3690.12863148647800715No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA3490.1216595901919363No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA3430.11956802130611505No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC3280.11433909909156191No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT3270.11399050427725838No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG3120.10876158206270523No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA3110.1084129872484017No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA3030.10562422873397335No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA3020.1052756339196698No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA3010.10492703910536627No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA2910.10144109096233084No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2900.1010924961480273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2800.0106.160711
CAAAAGC16750.080.940294
GAGCAAA17650.079.6883851
AGCAAAA17400.078.752
GCAAAAG17450.077.6934053
TTCGACT300.001934330272.55
AAAAGCA19000.072.118425
AAAGCAG23950.069.926936
AGCGAAA4450.066.797752
GCGAAAG4700.063.2446823
TTTCGAC350.003563911762.1428574
CGAAAGC5000.059.454
GCAGGAG7300.055.6164369
AAGCAGG30800.054.3757
CCACGTT400.00604650554.375145
AGCAGGA9950.050.2763828
ACAAGGT1457.2759576E-1250.08
AGAGCGA450.00963218448.3333326
CAAGGTA803.7279544E-545.31254
TACTATG2300.044.1304362
Produced by FastQC (version 0.11.2)