FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_2417_day_1_ul-b.3510000008bc22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_2417_day_1_ul-b.3510000008bc22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205283
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10670.5197702683612379No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG8110.395064374546358No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA7260.3536581207406361No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC6320.30786767535548487No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC5190.2528217144137605No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA3700.18023898715431869No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA3650.1778033251657468No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA3050.1485753813028843No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2850.13883273334859683No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2770.13493567416688182No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2470.12032170223545058No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2360.11496324586059245No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT2350.11447611346287807No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2320.11301471626973494No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC2240.10911765708801996No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2220.1081433922925912No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2160.10522059790630495No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2160.10522059790630495No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT2110.10278493591773308No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2100.1022978035200187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1650.0118.636371
GCAAAAG12150.079.958853
GAGCAAA12350.079.838061
CAAAAGC12400.078.346784
AGCAAAA12500.077.142
CATACCG300.001933543572.55
GCAGGGT3900.070.641039
GTAGAAA2700.069.814821
AAAAGCA14600.066.54115
GCAGGTA1550.065.483879
AAAGCAG18000.064.847226
AGCGAAA3150.064.444441
GCGAAAG3350.060.5970152
AAGCAGG19400.060.5412377
CGAAAGC3450.058.840583
CTGGTCG400.006044063354.3759
GCAGGGG4700.052.4468049
AGCAGGG14750.051.1186458
GGATAGC450.0096283148.3333325
GCAGGAG3250.046.8461539
Produced by FastQC (version 0.11.2)